Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,805,450	A→G	D53D (GAT→GAC)	unknown ←	Anaerobic respiratory reductase chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,805,450	0	A	G	100.0%	197.7 / NA	69	D53D (GAT→GAC)	unknown	Anaerobic respiratory reductase chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/39);  total (30/39)

GCTCAATGAATCGGTGTTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCATCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATACCCAGCTT  >  1/1805207‑1805696                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       gcTCAATGAATCGGTGTTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCg                                                                                                                                                                                                                                                     >  1:822044/1‑247 (MQ=255)        gAATCGGTGTTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGtt                                                                                                                                                                                                                                          >  1:555212/1‑251 (MQ=255)         aaTCGGTGTTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGttt                                                                                                                                                                                                                                         <  1:56267/251‑1 (MQ=255)             ggTGTTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACg                                                                                                                                                                                                                                     >  1:282387/1‑251 (MQ=255)              gtgtTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACg                                                                                                                                                                                                                                     >  1:384163/1‑250 (MQ=255)              gtgtTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACg                                                                                                                                                                                                                                     >  1:145189/1‑250 (MQ=255)              gtgtTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACg                                                                                                                                                                                                                                     >  1:301436/1‑250 (MQ=255)               tgtTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGc                                                                                                                                                                                                                                    <  1:822521/250‑1 (MQ=255)                gtTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGcc                                                                                                                                                                                                                                   >  1:247205/1‑250 (MQ=255)                gtTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGcc                                                                                                                                                                                                                                   >  1:178630/1‑250 (MQ=255)                    tCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGAc                                                                                                                                                                                                                              <  1:46975/251‑1 (MQ=255)                     cTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGAc                                                                                                                                                                                                                              >  1:174793/1‑250 (MQ=255)                         cAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTggg                                                                                                                                                                                                                         <  1:853348/251‑1 (MQ=255)                          aaCCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGa                                                                                                                                                                                                                        <  1:123843/251‑1 (MQ=255)                          aaCCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGa                                                                                                                                                                                                                        <  1:877849/251‑1 (MQ=255)                           aCCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGAt                                                                                                                                                                                                                       >  1:417806/1‑251 (MQ=255)                               ggAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAAt                                                                                                                                                                                                                   >  1:3785/1‑251 (MQ=255)                                     cGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCtt                                                                                                                                                                                                             <  1:515431/251‑1 (MQ=255)                                       tCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATCCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTtg                                                                                                                                                                                                            <  1:446510/250‑1 (MQ=255)                                           tAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCt                                                                                                                                                                                                              <  1:18349/244‑1 (MQ=255)                                           tAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTtgctg                                                                                                                                                                                                         <  1:569306/249‑1 (MQ=255)                                                 cATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGAc                                                                                                                                                                                                                              <  1:818287/222‑1 (MQ=255)                                                       aaaaTGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCaaaaa                                                                                                                                                                                           <  1:144722/251‑1 (MQ=255)                                                            gATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCg                                                                                                                                                                                      >  1:812393/1‑251 (MQ=255)                                                                 atatTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAAtt                                                                                                                                                                                                                  >  1:585474/1‑218 (MQ=255)                                                                 atatTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCt                                                                                                                                                                                                              <  1:147700/222‑1 (MQ=255)                                                                 atatTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATaa                                                                                                                                                                                 >  1:111335/1‑251 (MQ=255)                                                                     tttCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATg                                                                                                                                                                             <  1:645665/251‑1 (MQ=255)                                                                          gTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGc                                                                                                                                                                                        <  1:592642/235‑1 (MQ=255)                                                                           tttCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGTCCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATc                                                                                                                                                                        <  1:149754/250‑1 (MQ=255)                                                                             tCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGt                                                                                                                                                                     <  1:838108/251‑1 (MQ=255)                                                                                     aTTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGtt                                                                                                                                                                <  1:84450/248‑1 (MQ=255)                                                                                                        cccTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAAtt                                                                                                                                                                                                                  <  1:779191/179‑1 (MQ=255)                                                                                                        cccTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTAcc                                                                                                                                                                                                               <  1:821493/182‑1 (MQ=255)                                                                                                        cccTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGaa                                                                                                                                          >  1:275816/1‑251 (MQ=255)                                                                                                             aaaaTCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCg                                                                                                                                            <  1:687295/244‑1 (MQ=255)                                                                                                               aaTCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAGATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGTGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACgg                                                                                                                                   <  1:410789/251‑1 (MQ=255)                                                                                                                     cAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATc                                                                                                                             <  1:276340/251‑1 (MQ=255)                                                                                                                                                      ccACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGtt                                                                                                                                                                >  1:148783/1‑183 (MQ=255)                                                                                                                                                        acacTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCat                                                                                          <  1:881851/251‑1 (MQ=255)                                                                                                                                                           cTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAAt                                                                                                           >  1:720765/1‑231 (MQ=255)                                                                                                                                                           cTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCattat                                                                                       >  1:824018/1‑251 (MQ=255)                                                                                                                                                             tCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTAttt                                                                                     <  1:782101/251‑1 (MQ=255)                                                                                                                                                                      aCCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCAt                                                                             >  1:545372/1‑250 (MQ=255)                                                                                                                                                                      aCCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCAt                                                                             >  1:480221/1‑250 (MQ=255)                                                                                                                                                                              gTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATcc                                                                           <  1:70988/244‑1 (MQ=255)                                                                                                                                                                                             gATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTAttt                                                                                     >  1:150920/1‑219 (MQ=255)                                                                                                                                                                                              aTTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGAtt                                                    >  1:740087/1‑251 (MQ=255)                                                                                                                                                                                                    caaTGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTtg                                              <  1:701026/251‑1 (MQ=255)                                                                                                                                                                                                             aTCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCat                                                                                          >  1:196989/1‑198 (MQ=255)                                                                                                                                                                                                               cAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATc                                                                                                                             <  1:796399/161‑1 (MQ=255)                                                                                                                                                                                                                    ggTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTAttt                                                                                     <  1:365417/196‑1 (MQ=255)                                                                                                                                                                                                                     gTCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTAttt                                                                                     >  1:140070/1‑195 (MQ=255)                                                                                                                                                                                                                      tCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGtat                             <  1:585381/250‑1 (MQ=255)                                                                                                                                                                                                                      tCTTTGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGtat                             <  1:550036/250‑1 (MQ=255)                                                                                                                                                                                                                        tttGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGtattat                          >  1:339412/1‑251 (MQ=255)                                                                                                                                                                                                                         ttGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTattatt                         >  1:549700/1‑251 (MQ=255)                                                                                                                                                                                                                          tGCAAGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGACAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTc                        <  1:489838/251‑1 (MQ=255)                                                                                                                                                                                                                              aGGCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCaaaa                    >  1:137635/1‑251 (MQ=255)                                                                                                                                                                                                                                gCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTc                        <  1:569614/245‑1 (MQ=255)                                                                                                                                                                                                                                gCATCTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAAt                   <  1:265547/250‑1 (MQ=255)                                                                                                                                                                                                                                    cTAATGCGCTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATcc                                                                           <  1:77487/190‑1 (MQ=255)                                                                                                                                                                                                                                          cgcTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATAc         >  1:218989/1‑250 (MQ=255)                                                                                                                                                                                                                                          cgcTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATAc         <  1:569594/250‑1 (MQ=255)                                                                                                                                                                                                                                            cTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTAttt                                                                                     <  1:233866/172‑1 (MQ=255)                                                                                                                                                                                                                                            cTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATAccc       <  1:22214/250‑1 (MQ=255)                                                                                                                                                                                                                                            cTTTTACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATACCCa      >  1:274382/1‑251 (MQ=255)                                                                                                                                                                                                                                              tttACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATACCCAg     >  1:247942/1‑250 (MQ=255)                                                                                                                                                                                                                                                tACCGTCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATACCCAGCtt  <  1:430094/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GCTCAATGAATCGGTGTTATCTTCCAACCAGGAAGCCGTCAGTAGCAACATGGCAAAATGATCGATATTTTCCGTTTCAGGCAAATTGCGCATTTGCCGAAAACCCTTAAAATCTTCAATGGAAATCTCATAAGCAGAAATTTGTGTCGCCACACTTCCCGACTGACCGAAAAGTTTTTGATATTCTTGATTCAACAATGTCAAATCAATAGGTCTTTGCAAGGCATCTAATGCGCTTTTACCATCGCTGTCTTCGTTTAACGCCCAGACTTGGGATAAATTACCTTGCTGCAACCAAGCAAAAACGCCGGATAAAATGGGATCTGTCGGTTGGCGGTAAAATAAATTACCGAATAAACGGCTAATCAAAGAAAAATTATTGAATTGCACACTTTCCTGCATTATTTGGAGCCATCCTTTGCCAAAAGTGCGGTCAGATTTTCTTGTGTTTTTTGTGCGGTATTATTCAAAATCTGCTGATACCCAGCTT  >  1/1805207‑1805696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑