Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,796,180	A→G	L430P (CTG→CCG)	unknown ←	Bis‑ABC ATPase Uup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,796,180	0	A	G	100.0%	217.7 / NA	75	L430P (CTG→CCG)	unknown	Bis‑ABC ATPase Uup
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/38);  total (37/38)

GATGTAGCATTCGGTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCAGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGTTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTTAATAAAGGTGGTTTTGCCGCAGCCGTTCGGACCGA  >  1/1795942‑1796417                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              gATGTAGCATTCGGTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGGATAGGaaaa                                                                                                                                                                                                                                   >  1:821884/1‑250 (MQ=255) gATGTAGCATTCGGTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGaca                                                                                                                                                                                                                                    >  1:240476/1‑248 (MQ=255) gATGTAGCATTCGGTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGaaaa                                                                                                                                                                                                                                   >  1:395669/1‑250 (MQ=255) gATGTAGCATTCGGTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAAt                                                                                                                                                                                                                                   >  1:870260/1‑251 (MQ=255)              gTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGc                                                                                                                                                                                                                       >  1:128918/1‑250 (MQ=255)                   ggTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAaca                                                                                                                                                                                                                 >  1:490386/1‑251 (MQ=255)                    gTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAaca                                                                                                                                                                                                                 >  1:458442/1‑250 (MQ=255)                    gTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAaca                                                                                                                                                                                                                 >  1:26138/1‑250 (MQ=255)                     tattatCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAacac                                                                                                                                                                                                                <  1:830885/250‑1 (MQ=255)                             aTAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAacac                                                                                                                                                                                                                <  1:646668/242‑1 (MQ=255)                               aaaTTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAGTAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTa                                                                                                                                                                                                     <  1:247102/251‑1 (MQ=255)                                  ttGACGATCGTGACTGACGATAGGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGc                                                                                                                                                                                                  <  1:759991/251‑1 (MQ=255)                                       gATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAacac                                                                                                                                                                                                                <  1:106272/232‑1 (MQ=255)                                        aTCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGTATAGGAAATCCTGCAAGTAGGCCAACACATGATGTTTTACGCCggttt                                                                                                                                                                                            >  1:549698/1‑247 (MQ=255)                                        aTCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGa                                                                                                                                                                                            >  1:592626/1‑251 (MQ=255)                                        aTCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGa                                                                                                                                                                                            >  1:807256/1‑251 (MQ=255)                                               ctgacGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGAGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCcac                                                                                                                                                                                     <  1:157592/251‑1 (MQ=255)                                                  acGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCcac                                                                                                                                                                                     <  1:494070/248‑1 (MQ=255)                                                   cGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCaa                                                                                                                                                                                                                            <  1:60356/208‑1 (MQ=255)                                                      tAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTcc                                                                                                                                                                                       >  1:251493/1‑242 (MQ=255)                                                       aaGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGGTTTACGCCGGTGACTTCCACAt                                                                                                                                                                                   >  1:538965/1‑245 (MQ=255)                                                              gTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGt                                                                                                                                                                                                                          <  1:230512/199‑1 (MQ=255)                                                              gTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGt                                                                                                                                                                       >  1:152027/1‑250 (MQ=255)                                                              gTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGt                                                                                                                                                                       >  1:806896/1‑250 (MQ=255)                                                                 ccTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAacac                                                                                                                                                                                                                >  1:743660/1‑206 (MQ=255)                                                                   ttGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGGATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGa                                                                                                                                                                 >  1:674888/1‑251 (MQ=255)                                                                     gATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATg                                                                                                                                                                                                             >  1:590290/1‑205 (MQ=255)                                                                     gATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACg                                                                                                                                                               >  1:760321/1‑251 (MQ=255)                                                                           ccGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTtatct                                                                                                                                                         <  1:843253/251‑1 (MQ=255)                                                                             gTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTTATGGCACGTTTCGGCGGGTATAGGAAATCCTGGAAGGAGCCCAACACATGACGTTTTACGCCGTTGACCTCCACATCCTGTTTGCCGTTGGCGACTTtatctat                                                                                                                                                       >  1:799537/1‑251 (MQ=255)                                                                                                aTTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCg                                                                                                                                    >  1:423256/1‑251 (MQ=255)                                                                                                     aaGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATc                                                                                                                               <  1:256246/251‑1 (MQ=255)                                                                                                               cGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTAt                                                                                                                     <  1:517509/251‑1 (MQ=255)                                                                                                               cGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTAt                                                                                                                     <  1:245584/251‑1 (MQ=255)                                                                                                                    aaGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGt                                                                                                                 >  1:243060/1‑250 (MQ=255)                                                                                                                    aaGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTc                                                                                                                <  1:266871/251‑1 (MQ=255)                                                                                                                      gTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTc                                                                                                                <  1:893784/249‑1 (MQ=255)                                                                                                                       tCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTAt                                                                                                                     <  1:706532/243‑1 (MQ=255)                                                                                                                              tCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGc                                                                                                       <  1:772283/250‑1 (MQ=255)                                                                                                                              tCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGc                                                                                                       <  1:548653/250‑1 (MQ=255)                                                                                                                                      tCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTTCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCaa                                                                                              >  1:30460/1‑251 (MQ=255)                                                                                                                                           aaTGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAAttt                                                                                           <  1:98927/249‑1 (MQ=255)                                                                                                                                           aaTGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAAttt                                                                                           <  1:620984/249‑1 (MQ=255)                                                                                                                                           aaTGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAAttt                                                                                           <  1:209650/249‑1 (MQ=255)                                                                                                                                                   aaTTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTTCAATTTTGTGCCGCAg                                                                                 >  1:510494/1‑251 (MQ=255)                                                                                                                                                     ttattGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGt                                                                                                                                                                                                                          >  1:247443/1‑112 (MQ=255)                                                                                                                                                     ttattGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTtatctat                                                                                                                                                       <  1:671605/179‑1 (MQ=255)                                                                                                                                                     ttattGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTc                                                                                                                <  1:392746/218‑1 (MQ=255)                                                                                                                                                     ttattGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGGATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCg                                                                               >  1:593382/1‑251 (MQ=255)                                                                                                                                                     ttattGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGGCGAGATCAGCGCGGTAt                                                                                                                     >  1:774050/1‑213 (MQ=255)                                                                                                                                                     ttattGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGCAGCCCCACACATGACGTTTTACGCCGTTGACTTCCCCATCCTGTTTGCCGTCGGCGACGCTATCTATCACCGATTTTTCCGGGCCGAGATCAGCGCGGCATTGGCCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCg                                                                               >  1:77180/1‑251 (MQ=255)                                                                                                                                                          gggTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAg                                                                          >  1:95783/1‑251 (MQ=255)                                                                                                                                                           ggTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGa                                                                                                                                                                                                                                      >  1:60660/1‑94 (MQ=255)                                                                                                                                                             ttttAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAAc                                                                       <  1:412344/251‑1 (MQ=255)                                                                                                                                                                   cAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTtg                                                                 >  1:154536/1‑251 (MQ=255)                                                                                                                                                                        atTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGc                                                            <  1:21555/251‑1 (MQ=255)                                                                                                                                                                        atTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGc                                                            <  1:519309/251‑1 (MQ=255)                                                                                                                                                                                 aTAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTAt                                                                                                                     <  1:73302/185‑1 (MQ=255)                                                                                                                                                                                 aTAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCg                                                   >  1:799330/1‑251 (MQ=255)                                                                                                                                                                                  tAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTTCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTTGTc                                                                                                                >  1:256340/1‑189 (MQ=255)                                                                                                                                                                                  tAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGGTTTCTATTACCGATTTTTTCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCa                                                                                                      >  1:77976/1‑199 (MQ=255)                                                                                                                                                                                                     ccgccTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTtaataa                                <  1:619359/250‑1 (MQ=255)                                                                                                                                                                                                     ccgccTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTtaataa                                <  1:728732/250‑1 (MQ=255)                                                                                                                                                                                                     ccgccTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTtaataa                                <  1:801672/250‑1 (MQ=255)                                                                                                                                                                                                           gAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAgcg                                                                                                                           <  1:663579/153‑1 (MQ=255)                                                                                                                                                                                                           gAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTTAATAAAgg                             <  1:482663/247‑1 (MQ=255)                                                                                                                                                                                                               gggCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTtaa                                   <  1:587289/237‑1 (MQ=255)                                                                                                                                                                                                                ggCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTtatct                                                                                                                                                         >  1:412228/1‑118 (MQ=255)                                                                                                                                                                                                                ggCTTTAACCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGc                                                  <  1:372331/221‑1 (MQ=255)                                                                                                                                                                                                                      aaCCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCtt                                                                  <  1:662257/199‑1 (MQ=255)                                                                                                                                                                                                                      aaCCGGGGTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGc                                                  <  1:751570/215‑1 (MQ=255)                                                                                                                                                                                                                           gggTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATcc                                                                                                                                                                                 <  1:441326/83‑1 (MQ=255)                                                                                                                                                                                                                           gggTCATGGCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTTAATAAAggtgg                          <  1:469523/234‑1 (MQ=255)                                                                                                                                                                                                                                  ggCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTTAATAAAGGTGGTTTTGCCGCAGCCGTTCGGACCGa  >  1:627692/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCACGTTTCGGCGGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGGTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTTAATAAAGGTGGTTTTGCCGCAGCCGTTCGGACCGa  >  1:891754/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GATGTAGCATTCGGTGGCGGTATTATCAATAAATTGACGATCGTGACTGACGATAAGCAAGGTGCCTTGATAATCCGTGAGAATTTCTTCCAACAATTCCAAGGTTTCTACGTCCAAGTCATTAGTCGGCTCATCCAGAATGAGCAAATTATTGGGTTTTAACAATAATTTCGCCAATAACAGACGGTTACGTTCGCCGCCTGAAAGGGCTTTAACCGGGGTCATGGCACGTTTCGGCAGGAATAGGAAATCCTGCAAGTAGCCCAACACATGACGTTTTACGCCGTTGACTTCCACATCCTGTTTGCCGTCGGCGACGTTATCTATCACCGATTTTTCCGGTTCGAGATCAGCGCGGTATTGGTCAAAATAGGCAATGTCCAATTTTGTGCCGCAGCGAATAGAACCGCTTGTGGGCTGAATCTCGCCGAGCAACAATTTAATAAAGGTGGTTTTGCCGCAGCCGTTCGGACCGA  >  1/1795942‑1796417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑