Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,794,646	G→T	L207I (CTT→ATT)	unknown ←	FIG00781545: hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,794,646	0	G	T	100.0%	59.7 / NA	23	L207I (CTT→ATT)	unknown	FIG00781545: hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/12);  total (11/12)

GATTTCCCTTCCTTGACATATCCCAAATTTAACCCCATAAACTTTTTATATATTATAGGACAATTTTCCTGTTTACTTTGGATAATTTCCTTTAAAAACATTTCACGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAAGTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAGATATAAAACTTCTCAATAAACTTGAATAATTATTTTTATTATTTAACTCACCATTTTCATAAATGAATTTCAAAATCCTATATAGAAGTAAAAA  >  1/1794408‑1794862                                                                                                                                                                                                                                               |                                                                                                                                                                                                                         gATTTCCCTTCCTTGACATATCCCAAATTTAACCCCATAAACTTTTTATATATTATAGGACAATTTTCCTGTTTACTTTGGATAATTTCCTTTAAAAACATTTCACGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCtat                                                                                                                                                                                                              >  1:230984/1‑251 (MQ=255)                                           tttttATATATTATAGGACAATTTTCCTGTTTACTTTGGATAATTGCCTTTAAAAACAGTTCACGATCATAGCTATCAATAGCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCATTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTc                                                                                                                                                                    <  1:449089/251‑1 (MQ=255)                                                  atatTATAGGACAATTTTCCTGTTTACTTTGGATAATTTCCTTTAAAAACATTTCACGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATaa                                                                                                                                                             >  1:695877/1‑251 (MQ=255)                                                     ttatAGGACAATTTTCCTGTTTACTTTGGATAATTTCCTTTAAAAACATTTCACGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAat                                                                                                                                                                          >  1:760587/1‑235 (MQ=255)                                                          ggACAATTTTCCTGTTTACTTTGGATAATTTCCTTTAAAAACATTTCACGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTc                                                                                                                                                          >  1:706028/1‑246 (MQ=255)                                                                                                          cGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAagag                                                                                                                                                 <  1:179127/207‑1 (MQ=255)                                                                                                              cATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTc                                                                                                                                                          <  1:198870/194‑1 (MQ=255)                                                                                                                tAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCAtct                                                                                                                                           >  1:497814/1‑207 (MQ=255)                                                                                                                        aaTATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAGATATAAAAc                                                                                       <  1:107305/251‑1 (MQ=255)                                                                                                                                   gAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAagag                                                                                                                                                 <  1:674003/182‑1 (MQ=255)                                                                                                                                      ttaattCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTc                                                                                                                                                                    <  1:470086/160‑1 (MQ=255)                                                                                                                                                 ctctTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAata                                                                                                                                                                         <  1:320204/144‑1 (MQ=255)                                                                                                                                                 ctctTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATtagat                                                                                                                                  <  1:657039/183‑1 (MQ=255)                                                                                                                                                 ctctTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAGATATAAAACTTCTCAATAAACTTGAATAattatt                                                              >  1:233932/1‑251 (MQ=255)                                                                                                                                                                        ccTTTCTCCCTTAAAAACTAAATCGTATAACGCTTCTTTCTCTACCCTCCTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAGATATAAAACTTCTCAATAAACTTGAATAATTATTTTTATTATTTAACTc                                               <  1:319961/242‑1 (MQ=255)                                                                                                                                                                        aCTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAata                                                                                                                                                                         <  1:677588/121‑1 (MQ=255)                                                                                                                                                                               cccTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTc                                                                                                                                                                    >  1:351771/1‑119 (MQ=255)                                                                                                                                                                                                gTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAg                                                                                                >  1:482047/1‑170 (MQ=255)                                                                                                                                                                                                 tataAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTc                                                                                                                                                          <  1:88858/111‑1 (MQ=255)                                                                                                                                                                                                            tttctCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAatat                                                                                                                                                               >  1:490459/1‑95 (MQ=255)                                                                                                                                                                                                             ttctCTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAGATATAAAACTTCTCAATAAACTTGAATAATTATTTTTATTATTTAACTCACCATTTTCATAAATGAATTTCAAAATCCTATATAGAAGTaaaaa  >  1:620099/1‑251 (MQ=255)                                                                                                                                                                                                              tctcTACCCTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTa                                                                                                                                                                            <  1:710591/80‑1 (MQ=255)                                                                                                                                                                                                                      cTACTCACTTGATGTATAAATTCAATTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAatatat                                                                                                                                                                      >  1:783170/1‑78 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                         GATTTCCCTTCCTTGACATATCCCAAATTTAACCCCATAAACTTTTTATATATTATAGGACAATTTTCCTGTTTACTTTGGATAATTTCCTTTAAAAACATTTCACGATCATAGCTATCAATATCATCAGGAATTAATTCACCACTCTTAAGAGCATCAAATAGATAACTTTCTCCCTTAAAAACTAAATCGTATAAAGCTTCTTTCTCTACCCTACTCACTTGATGTATAAATTCAAGTTCTAGATCTATAAAATTTACGTGCTCCAAAAATGAGAACCTATTAATATATTCAATATAATTCCTATAAGAGTCATCTCTATTAGATAGATGATAAGACAATACAACTAATAATTTATTAGATATAAAACTTCTCAATAAACTTGAATAATTATTTTTATTATTTAACTCACCATTTTCATAAATGAATTTCAAAATCCTATATAGAAGTAAAAA  >  1/1794408‑1794862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑