Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,783,035	T→C	D92D (GAT→GAC)	unknown →	Manganese ABC transporter, inner membrane permease protein SitC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,783,035	0	T	C	100.0%	217.6 / NA	75	D92D (GAT→GAC)	unknown	Manganese ABC transporter, inner membrane permease protein SitC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

ATTACATGGTGAAAGCCATTTTAATCAGCTCGGCGGTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGATGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGCCACTTCCGTCGGCTTTTCAC  >  1/1782794‑1783276                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  aTTACATGGTGAAAGCCATTTTAATCAGCTCGGCGGTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGATGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCAt                                                                                                                                                                                                                                           >  1:145372/1‑250 (MQ=255)       tGGTGAAAGCCATTTTAATCAGCTCGGCGGTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCttt                                                                                                                                                                                                                                    >  1:7027/1‑251 (MQ=255)                  aTTTTAATCAGCTCGGCGGTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTcaccac                                                                                                                                                                                                                         <  1:131867/251‑1 (MQ=255)                             cTCGGCGGTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGccc                                                                                                                                                                                                              <  1:667653/251‑1 (MQ=255)                                  cggTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGc                                                                                                                                                                                                         <  1:777112/251‑1 (MQ=255)                                       ggtggCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTAc                                                                                                                                                                                                     <  1:688005/250‑1 (MQ=255)                                         tggCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTg                                                                                                                                                                                                   <  1:618037/250‑1 (MQ=255)                                         tggCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTg                                                                                                                                                                                                   <  1:186288/250‑1 (MQ=255)                                         tggCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGa                                                                                                                                                                                                  <  1:570373/251‑1 (MQ=255)                                         tggCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGa                                                                                                                                                                                                  <  1:690778/251‑1 (MQ=255)                                                tgtgCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTc                                                                                                                                                                                           <  1:312278/251‑1 (MQ=255)                                                    cGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCtt                                                                                                                                                                                       <  1:307845/251‑1 (MQ=255)                                                        tttATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAAcc                                                                                                                                                                                   <  1:878111/251‑1 (MQ=255)                                                                   aTTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGAtt                                                                                                                                                                        >  1:581549/1‑251 (MQ=255)                                                                    tttAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTa                                                                                                                                                                       >  1:112142/1‑251 (MQ=255)                                                                          gCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTa                                                                                                                                                                  >  1:78238/1‑250 (MQ=255)                                                                          gCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTa                                                                                                                                                                  >  1:802446/1‑250 (MQ=255)                                                                           cTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACa                                                                                                                                                                >  1:796214/1‑251 (MQ=255)                                                                              aaaGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAg                                                                                                                                                              <  1:583658/250‑1 (MQ=255)                                                                               aaGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGAc                                                                                                                                                            <  1:425445/251‑1 (MQ=255)                                                                                 ggTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGc                                                                                                                                                                                                         <  1:304606/204‑1 (MQ=255)                                                                                       tCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCt                                                                                                                                                     <  1:801065/250‑1 (MQ=255)                                                                                          cTGATTGGTGATGCCCTTTCTCATTCCGTCGCACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAgg                                                                                                                                                 >  1:302237/1‑251 (MQ=255)                                                                                            gATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCt                                                                                                                                                     <  1:475915/245‑1 (MQ=255)                                                                                              ttGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAAc                                                                                                                                             <  1:525298/251‑1 (MQ=255)                                                                                                 gTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTAttt                                                                                                                                                                                                                                >  1:828026/1‑165 (MQ=255)                                                                                                 gTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACAt                                                                                                                                           <  1:575091/250‑1 (MQ=255)                                                                                                  tGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTc                                                                                                                                         <  1:285108/251‑1 (MQ=255)                                                                                                      gCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTggg                                                                                                                                     >  1:694354/1‑251 (MQ=255)                                                                                                       cccTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTAtt                                                                                                                                                                                                                                 <  1:42559/158‑1 (MQ=255)                                                                                                       cccTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGc                                                                                                                                    <  1:2158/251‑1 (MQ=255)                                                                                                       cccTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGc                                                                                                                                    <  1:613049/251‑1 (MQ=255)                                                                                                       cccTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGATATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTAt                                                                                                                                                       <  1:73239/232‑1 (MQ=255)                                                                                                        ccTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCt                                                                                                                                                     >  1:572488/1‑233 (MQ=255)                                                                                                           ttCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTg                                                                                                                                >  1:319741/1‑251 (MQ=255)                                                                                                           ttCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTACCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATc                                                                                                                                                      >  1:442359/1‑229 (MQ=255)                                                                                                                   ccGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGcc                                                                                                                                                                                                        <  1:665070/171‑1 (MQ=255)                                                                                                                   ccGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGt                                                                                                                                                                             <  1:357835/198‑1 (MQ=255)                                                                                                                    cgtcgtACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAg                                                                                                                       <  1:783534/251‑1 (MQ=255)                                                                                                                     gtcgtACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTc                                                                                                                                         <  1:497095/232‑1 (MQ=255)                                                                                                                             cGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGc                                                                                                              <  1:667826/251‑1 (MQ=255)                                                                                                                                  ttGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGc                                                                                                                                    <  1:10438/224‑1 (MQ=255)                                                                                                                                    gCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATc                                                                                                                                                      >  1:1406/1‑204 (MQ=255)                                                                                                                                          gCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTg                                                                                                                                                                                                   >  1:55666/1‑153 (MQ=255)                                                                                                                                          gCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTat                                                                                                 >  1:758050/1‑251 (MQ=255)                                                                                                                                          gCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTat                                                                                                 >  1:46069/1‑251 (MQ=255)                                                                                                                                            ttATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTatca                                                                                               <  1:108158/251‑1 (MQ=255)                                                                                                                                               tGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGc                                                                                                                                                                                                         <  1:17182/142‑1 (MQ=255)                                                                                                                                                gCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCgg                                                                                           >  1:802214/1‑251 (MQ=255)                                                                                                                                                     ctcCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTg                                                                                                                                                                                                   >  1:393602/1‑142 (MQ=255)                                                                                                                                                     ctcCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGa                                                                                                                                                                                                  >  1:346570/1‑143 (MQ=255)                                                                                                                                                     ctcCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATc                                                                                                                                                      >  1:399465/1‑187 (MQ=255)                                                                                                                                                     ctcCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCgttt                                                                                      >  1:789951/1‑251 (MQ=255)                                                                                                                                                       cccTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACCTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGc                                                                                                                                    <  1:735561/203‑1 (MQ=255)                                                                                                                                                           gCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTc                                                                                                                 >  1:709784/1‑218 (MQ=255)                                                                                                                                                           gCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCgtttgttt                                                                                  >  1:748656/1‑249 (MQ=255)                                                                                                                                                              cTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTAt                                                                             <  1:101772/251‑1 (MQ=255)                                                                                                                                                              cTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTAt                                                                             <  1:546239/251‑1 (MQ=255)                                                                                                                                                                     cTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTActgc                                                                       <  1:855329/250‑1 (MQ=255)                                                                                                                                                                         gTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGc                                                                                                                                    >  1:33722/1‑185 (MQ=255)                                                                                                                                                                             aTTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAg                                                                                                                       >  1:292065/1‑194 (MQ=255)                                                                                                                                                                                                   gccatcgccatTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTActgc                                                                       >  1:810351/1‑220 (MQ=255)                                                                                                                                                                                                       tcgccatTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCt                                                                                                                                                     >  1:846159/1‑138 (MQ=255)                                                                                                                                                                                                       tcgccatTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGc                                                                                          <  1:503526/197‑1 (MQ=255)                                                                                                                                                                                                       tcgccatTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCttt                                    >  1:621334/1‑251 (MQ=255)                                                                                                                                                                                                                      tAAAATCCATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGcc                     <  1:131170/251‑1 (MQ=255)                                                                                                                                                                                                                            ccATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGCCACTTc                >  1:859734/1‑250 (MQ=255)                                                                                                                                                                                                                             cATTACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGCCACTTCCg              <  1:142056/251‑1 (MQ=255)                                                                                                                                                                                                                               ttACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGattat                                                                                                                                                                                              >  1:104528/1‑73 (MQ=255)                                                                                                                                                                                                                               ttACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTc                                                                                                                                                                                           <  1:262546/76‑1 (MQ=255)                                                                                                                                                                                                                               ttACCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGcc                     >  1:488965/1‑242 (MQ=255)                                                                                                                                                                                                                                 aCCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTg                                                                                                               <  1:375161/150‑1 (MQ=255)                                                                                                                                                                                                                                 aCCAAACTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGc                      <  1:770978/239‑1 (MQ=255)                                                                                                                                                                                                                                       cTCCGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTtgatg                                >  1:422497/1‑223 (MQ=255)                                                                                                                                                                                                                                          cGTGAAGACGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGCCACTTCCGTCGGCTTTTCAc  >  1:1851/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  ATTACATGGTGAAAGCCATTTTAATCAGCTCGGCGGTGGGTGGCATTTGTGCGTTTTTATCCGCTTATTTAATGCTGAAAGGTTGGTCACTGATTGGTGATGCCCTTTCTCATTCCGTCGTACCCGGCGTTGCCATTGCTTATGCCCTCTCCCTGCCCTACGCGCTTGGTGCATTTTTTTCCGGTATCCTCGCCGCCATCGCCATTCTTTGGATAAAATCCATTACCAAACTCCGTGAAGATGCAGTCATCGGCTTTATTTTTACCACTTTCTTTGCCCTTGGCCTACTGATTATTTCCCTTAACCCGACGTCGATTAACGTACAAGACATTATCTTAGGCAACATTCTGGGCATTGCCGATGAAGATATTCTGCAAGTCGGCATTATCATCGGCGTTTGTTTAGTATTACTGCTGATCTTTTGGAAAGATCTTTTGCTGATTTTCTTTGATGAAGTGCACGCCACTTCCGTCGGCTTTTCAC  >  1/1782794‑1783276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑