Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	247,828	C→A	E123* (GAG→TAG)	unknown ←	Transposase, IS200 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	247,828	0	C	A	100.0%	16.5 / NA	9	E123* (GAG→TAG)	unknown	Transposase, IS200 family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/6);  total (3/6)

TTAAACATGGTAGAAGAAGACAATCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCGGTTTTTAGGCTACTCTATAAGGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATCCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTCCACTGTCTTTGTATTTAGCCCTACCGTGCTTA  >  1/247581‑247860                                                                                                                                                                                                                                                        |                                 ttAAACATGGTAGAAGAAGACAATCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTacac                               <  1:575243/251‑1 (MQ=255) ttAAACATGGTAGAAGAAGACAATCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTacac                               <  1:874880/251‑1 (MQ=255)        tGGTAGAAGAAGACAATCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTATTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACtacc                           <  1:429235/248‑4 (MQ=255)                 aagaCAATCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGc               <  1:73772/251‑1 (MQ=255)                      aaTCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTAc          <  1:299459/251‑1 (MQ=255)                      aaTCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTAc          <  1:86947/251‑1 (MQ=255)                           cgccgAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGCCCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATACCTCTATGCCCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGACTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTACCGTGc     <  1:343786/247‑1 (MQ=11)                               gAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGcc              >  1:409492/1‑238 (MQ=11)                               gAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGc               <  1:886561/237‑1 (MQ=11)                               gAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTACCGTGCTTa  >  1:298880/1‑250 (MQ=255)                               gAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCAGTTTTTAGGCTACTCTATAAAGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATTCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTACACTACCTTTGTATTTAGCCCTACCGTGCTTa  >  1:74886/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                 TTAAACATGGTAGAAGAAGACAATCACGCGGAATAAGATTCCTGTAAATAACAATCCGCACGTAAAACGTGCGGTTTTTAGGCTACTCTATAAGGACCTAGTACTTCACATGCCCTTCAAAGGGCATATGAAAACCAACAACCGTGAAATAACTCTATGACCTACCCCTTAAAGGGGTCCACATATCCTTTCTTCGATAAATTATCCTGAATCATGTCTTCCTTTTCCTGATTCCTTATATACTCCTCCACTGTCTTTGTATTTAGCCCTACCGTGCTTA  >  1/247581‑247860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑