Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,772,741	C→T	G158G (GGC→GGT)	unknown →	5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,772,741	0	C	T	100.0%	215.1 / NA	74	G158G (GGC→GGT)	unknown	5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/29);  total (45/29)

ATTCCGGCTCGTTTCGGCTTTGACAGCCAACATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGCCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCA  >  1/1772502‑1772982                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  aTTCCGGCTCGTTTCGGCTTTGACAGCCAACATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACg                                                                                                                                                                                                                                          <  1:506111/249‑1 (MQ=255)        cTCGTTTCGGCTTTGACAGCCAACATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTgg                                                                                                                                                                                                                                 <  1:513509/251‑1 (MQ=255)          cGTTTCGGCTTTGACAGCCAACATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGtt                                                                                                                                                                                                                               <  1:763005/251‑1 (MQ=255)                              aCATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCgg                                                                                                                                                                                                           >  1:171696/1‑251 (MQ=255)                               cATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTgg                                                                                                                                                                                                                                 >  1:368356/1‑228 (MQ=255)                               cATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCgg                                                                                                                                                                                                           >  1:159831/1‑250 (MQ=255)                                aTTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCa                                                                                                                                                                                                         >  1:212787/1‑251 (MQ=255)                                 tttAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCCAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCAt                                                                                                                                                                                                        >  1:440044/1‑251 (MQ=255)                                           gATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCtt                                                                                                                                                                                              >  1:873217/1‑251 (MQ=255)                                           gATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCtt                                                                                                                                                                                              >  1:668099/1‑251 (MQ=255)                                              cAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACG‑TCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGAt                                                                                                                                                                                          >  1:610471/1‑251 (MQ=255)                                                atatTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGc                                                                                                                                                                                                                           <  1:2286/217‑1 (MQ=255)                                                atatTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGAtt                                                                                                                                                                                         <  1:545618/251‑1 (MQ=255)                                                     tCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAAt                                                                                                                                                                                                   >  1:365409/1‑236 (MQ=255)                                                          cTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATtaaataa                                                                                                                                                                               >  1:780388/1‑251 (MQ=255)                                                              cgcgcgGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACt                                                                                                                                                                            <  1:4621/250‑1 (MQ=255)                                                                             cacaATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAGGCCCTTGGCTTGAATGTTAAACCCTCTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                             <  1:612866/250‑1 (MQ=255)                                                                             cacaATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                             <  1:710883/250‑1 (MQ=255)                                                                             cacaATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                             <  1:642854/250‑1 (MQ=255)                                                                                         ttGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCTGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAAc                                                                                                                                                <  1:432999/251‑1 (MQ=255)                                                                                         ttGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAAc                                                                                                                                                <  1:15001/251‑1 (MQ=255)                                                                                            aaaTGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGc                                                                                                                                              <  1:472863/250‑1 (MQ=255)                                                                                                gACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTgg                                                                                                                                         >  1:790637/1‑251 (MQ=255)                                                                                                 aCCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGaaa                                                                                                                                                        >  1:849431/1‑235 (MQ=255)                                                                                                     aaTGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                             <  1:56846/226‑1 (MQ=255)                                                                                                         gTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAg                                                                                                                                >  1:278470/1‑251 (MQ=255)                                                                                                             gACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGt                                                                                                                            >  1:522571/1‑251 (MQ=255)                                                                                                               cacCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGtt                                                                                                                                                                                                                               <  1:397111/150‑1 (MQ=255)                                                                                                                 ccAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGAt                                                                                                                        <  1:681631/251‑1 (MQ=255)                                                                                                                     cTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATtata                                                                                                                    >  1:587453/1‑251 (MQ=255)                                                                                                                       aTCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATAtt                                                                                                                  >  1:398072/1‑251 (MQ=255)                                                                                                                        tCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATAtt                                                                                                                  >  1:692801/1‑250 (MQ=255)                                                                                                                        tCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTc                                                                                                                 <  1:167910/251‑1 (MQ=255)                                                                                                                                    gCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTc                                                                                                                 >  1:862898/1‑239 (MQ=255)                                                                                                                                     ccGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAAc                                                                                                     >  1:327562/1‑250 (MQ=255)                                                                                                                                                cgcgGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTaa                                                                                            >  1:601303/1‑248 (MQ=255)                                                                                                                                                    gATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGa                                                                                     >  1:703876/1‑251 (MQ=255)                                                                                                                                                      tACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGaa                                                                                                                                                                                                                     >  1:169121/1‑121 (MQ=255)                                                                                                                                                       aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                             >  1:676412/1‑176 (MQ=255)                                                                                                                                                       aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTc                                                                                                                 <  1:269404/220‑1 (MQ=255)                                                                                                                                                       aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGAttt                                                                                  >  1:79766/1‑251 (MQ=255)                                                                                                                                                       aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGAttt                                                                                  >  1:345573/1‑251 (MQ=255)                                                                                                                                                             ttCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCgg                                                                             >  1:169944/1‑250 (MQ=255)                                                                                                                                                                  aGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAAc                                                                                           <  1:394019/231‑1 (MQ=255)                                                                                                                                                                  aGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAAt                                                                       >  1:81138/1‑251 (MQ=255)                                                                                                                                                                  aGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAAt                                                                       >  1:20490/1‑251 (MQ=255)                                                                                                                                                                    cTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                             <  1:788211/163‑1 (MQ=255)                                                                                                                                                                    cTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATg                                                                      <  1:575479/250‑1 (MQ=255)                                                                                                                                                                           ggCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCgg                                                              >  1:812639/1‑251 (MQ=255)                                                                                                                                                                               cATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTta                                                          >  1:427464/1‑251 (MQ=255)                                                                                                                                                                                aTTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGCGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTtat                                                         >  1:371009/1‑251 (MQ=255)                                                                                                                                                                                        caacaaATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACg                                                  <  1:69881/250‑1 (MQ=255)                                                                                                                                                                                        caacaaATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACg                                                  <  1:209327/250‑1 (MQ=255)                                                                                                                                                                                              aTTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGc                                           <  1:782609/251‑1 (MQ=255)                                                                                                                                                                                                       gCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGa                                                    >  1:73587/1‑233 (MQ=255)                                                                                                                                                                                                       gCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGCGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAg                                  >  1:560285/1‑251 (MQ=255)                                                                                                                                                                                                        ccAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAg                                  <  1:892985/250‑1 (MQ=255)                                                                                                                                                                                                        ccAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGc                                 <  1:519061/251‑1 (MQ=255)                                                                                                                                                                                                        ccAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGc                                 <  1:247277/251‑1 (MQ=255)                                                                                                                                                                                                             gCCCTTGGCTTGAATGTTAAACCCGCTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGcc                                          >  1:83573/1‑237 (MQ=255)                                                                                                                                                                                                             gCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTc                                                                                                                                                                           >  1:146774/1‑108 (MQ=255)                                                                                                                                                                                                               ccTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAATCACACAAg                          <  1:644327/251‑1 (MQ=255)                                                                                                                                                                                                                   ggCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCtt                                                                                               >  1:776704/1‑178 (MQ=255)                                                                                                                                                                                                                          aTGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCaaaa               >  1:772916/1‑251 (MQ=255)                                                                                                                                                                                                                          aTGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCaaaa               >  1:621918/1‑251 (MQ=255)                                                                                                                                                                                                                          aTGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCaaaa               >  1:196333/1‑251 (MQ=255)                                                                                                                                                                                                                                 aCCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTa                        >  1:886382/1‑235 (MQ=255)                                                                                                                                                                                                                                 aCCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTa            >  1:71176/1‑247 (MQ=255)                                                                                                                                                                                                                                   ccACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTa      >  1:260338/1‑251 (MQ=255)                                                                                                                                                                                                                                      cTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGcc                                          >  1:434701/1‑212 (MQ=255)                                                                                                                                                                                                                                      cTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGc                   >  1:256335/1‑235 (MQ=255)                                                                                                                                                                                                                                        aTTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGAt                                                                                                                        <  1:707453/132‑1 (MQ=255)                                                                                                                                                                                                                                         ttgttgGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACg                                                  <  1:113887/201‑1 (MQ=255)                                                                                                                                                                                                                                         ttgttgGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGCGCGGCATTTAATCGCTTTGATTTATTAAATAACCTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGTCTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCa  >  1:531316/1‑249 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  ATTCCGGCTCGTTTCGGCTTTGACAGCCAACATTTAACCCTTGATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGCCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCA  >  1/1772502‑1772982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑