Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,757,807	T→C	I29T (ATA→ACA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,757,807	0	T	C	100.0%	228.1 / NA	74	I29T (ATA→ACA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/33);  total (41/33)

TATCTGCGAAGTGGATGACGAAGAAAGTGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAATACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCACTGACTGCCACGATG  >  1/1757585‑1758051                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     tATCTGCGAAGTGGATGACGAAGAAAGTGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAAct                                                                                                                                                                                                                           >  1:495038/1‑250 (MQ=255) tATCTGCGAAGTGGATGACGAAGAAAGTGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAAct                                                                                                                                                                                                                           >  1:649552/1‑250 (MQ=255)                tGACGAAGAAAGTGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCg                                                                                                                                                                                                           <  1:499548/251‑1 (MQ=255)                         aaGTGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACg                                                                                                                                                                                                  <  1:619976/251‑1 (MQ=255)                            tGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGac                                                                                                                                                                                               <  1:520629/251‑1 (MQ=255)                                         gAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTAttt                                                                                                                                                                                  >  1:145460/1‑251 (MQ=255)                                           aaGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTg                                                                                                                                                                                <  1:717480/251‑1 (MQ=255)                                                  gTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTCGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGgtg                                                                                                                                                                         >  1:673261/1‑251 (MQ=255)                                                          tAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATg                                                                                                                                                                 <  1:324974/251‑1 (MQ=255)                                                          tAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATg                                                                                                                                                                 >  1:588009/1‑251 (MQ=255)                                                              gaagaaTAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAACACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTAt                                                                                                                                                             >  1:454800/1‑251 (MQ=255)                                                                 gaaTAGCAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAAt                                                                                                                                                                                                                  <  1:835240/195‑1 (MQ=255)                                                                 gaaTAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCa                                                                                                                                                                   >  1:306509/1‑242 (MQ=255)                                                                 gaaTAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGCGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTtg                                                                                                                                                          >  1:391987/1‑251 (MQ=255)                                                                    tAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATc                                                                                                                                                                                                                 >  1:280119/1‑193 (MQ=255)                                                                       cAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTtg                                                                                                                                                          >  1:5960/1‑245 (MQ=255)                                                                         aGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGGCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCTCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTc                                                                                                                                                     <  1:554926/248‑1 (MQ=255)                                                                         aGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAAc                                                                                                                                                                                                   <  1:5393/202‑1 (MQ=255)                                                                           aTTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTa                                                                                                                                                <  1:805505/251‑1 (MQ=255)                                                                           aTTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTa                                                                                                                                                >  1:505604/1‑251 (MQ=255)                                                                                         aTTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAAttt                                                                                                                                  >  1:833895/1‑251 (MQ=255)                                                                                                       gtgtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAAt                                                                                                                                                                                                                  <  1:181950/157‑1 (MQ=255)                                                                                                       gtgtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCa                                                                                                                                                                   >  1:872784/1‑204 (MQ=255)                                                                                                         gtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAAt                                                                                                                                                                                                                  >  1:670285/1‑155 (MQ=255)                                                                                                         gtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCa                                                                                                                                                                   <  1:160135/202‑1 (MQ=255)                                                                                                         gtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAAc                                                                                                                                                  <  1:114037/219‑1 (MQ=255)                                                                                                         gtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAAt                                                                                                                  >  1:605405/1‑251 (MQ=255)                                                                                                         gtTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAAt                                                                                                                  >  1:574089/1‑251 (MQ=255)                                                                                                                    gCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTg                                                                                                           >  1:130275/1‑247 (MQ=255)                                                                                                                    gCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTg                                                                                                       >  1:413112/1‑251 (MQ=255)                                                                                                                        ttACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAgg                                                                                                   <  1:503107/251‑1 (MQ=255)                                                                                                                          aCTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCt                                                                                                 <  1:213175/251‑1 (MQ=255)                                                                                                                           cTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTa                                                                                                                                                >  1:360578/1‑203 (MQ=255)                                                                                                                           cTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTa                                                                                                >  1:640296/1‑251 (MQ=255)                                                                                                                                 gAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTg                                                                                           <  1:323439/250‑1 (MQ=255)                                                                                                                                    tGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGtct                                                                                        <  1:116630/250‑1 (MQ=255)                                                                                                                                    tGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGtct                                                                                        <  1:167231/250‑1 (MQ=255)                                                                                                                                      aaaaaTGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGtcttc                                                                                      <  1:499208/250‑1 (MQ=255)                                                                                                                                              gTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTAcc                                                                                                                                       <  1:339574/193‑1 (MQ=255)                                                                                                                                                 tttATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTtg                                                                                                                                                          <  1:217407/171‑1 (MQ=255)                                                                                                                                                         gTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACgg                                                                                                                                                                                                 <  1:215503/124‑1 (MQ=255)                                                                                                                                                             aTTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTAt                                                                                                                                                                                    >  1:471937/1‑133 (MQ=255)                                                                                                                                                              tttGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAAc                                                             <  1:690449/251‑1 (MQ=255)                                                                                                                                                                      ttgtttgtGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAAc                                                                                                                                                                                                   >  1:407275/1‑109 (MQ=255)                                                                                                                                                                      ttgtttgtGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACAtt                                                                                            >  1:800496/1‑212 (MQ=255)                                                                                                                                                                      ttgtttgtGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCaa                                                     >  1:358403/1‑251 (MQ=255)                                                                                                                                                                      ttgtttgtGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCaa                                                     >  1:55412/1‑251 (MQ=255)                                                                                                                                                                       tgtttgtGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGtc                                                                                         <  1:593914/214‑1 (MQ=255)                                                                                                                                                                                        gTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGac                                                                                                                                                                                               >  1:172738/1‑95 (MQ=255)                                                                                                                                                                                        gTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACttttt                                    >  1:376832/1‑250 (MQ=255)                                                                                                                                                                                           tAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTttcttc                                <  1:300202/251‑1 (MQ=255)                                                                                                                                                                                                 tgttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTAttt                                                                                                                                                                                  <  1:595984/99‑1 (MQ=255)                                                                                                                                                                                                 tgttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTAttt                                                                                                                                                                                  >  1:254328/1‑99 (MQ=255)                                                                                                                                                                                                 tgttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAAc                                                                                                                                                  <  1:311469/131‑1 (MQ=255)                                                                                                                                                                                                 tgttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGc                           >  1:786476/1‑250 (MQ=255)                                                                                                                                                                                                  gttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGcc                          >  1:375861/1‑250 (MQ=255)                                                                                                                                                                                                  gttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGcc                          >  1:697372/1‑250 (MQ=255)                                                                                                                                                                                                   ttAATTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCt                        <  1:525968/251‑1 (MQ=255)                                                                                                                                                                                                      aTTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATc                                                                                                                             <  1:294263/147‑1 (MQ=255)                                                                                                                                                                                                      aTTGTGCGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTg                      >  1:62581/1‑250 (MQ=255)                                                                                                                                                                                                            cGTGGGGCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCac                <  1:48852/250‑1 (MQ=255)                                                                                                                                                                                                                gggCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTtg                                                                                                                                                          >  1:75325/1‑108 (MQ=255)                                                                                                                                                                                                                gggCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCactga             >  1:688576/1‑249 (MQ=255)                                                                                                                                                                                                                gggCAATGCAGTTAACACAGAAAAACTGGCGCGCGGAAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCactga             >  1:291540/1‑249 (MQ=255)                                                                                                                                                                                                                  gCAATGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGc                                                                                 >  1:821158/1‑179 (MQ=255)                                                                                                                                                                                                                     aTGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATc                                                                                                                             >  1:660526/1‑132 (MQ=255)                                                                                                                                                                                                                     aTGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTg                                                                                                           <  1:559126/150‑1 (MQ=255)                                                                                                                                                                                                                     aTGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAAc                                                             <  1:73868/196‑1 (MQ=255)                                                                                                                                                                                                                     aTGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCACTGACTGCCa       >  1:865549/1‑250 (MQ=255)                                                                                                                                                                                                                      tGCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCACTGACTGCCACg     >  1:361221/1‑251 (MQ=255)                                                                                                                                                                                                                       gCAGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTg                                                                                                                                                                                >  1:611913/1‑79 (MQ=255)                                                                                                                                                                                                                         aGTTAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTg                                                                                                           >  1:176148/1‑146 (MQ=255)                                                                                                                                                                                                                           ttAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCACTGACTGCCACGATg  <  1:205418/249‑1 (MQ=255)                                                                                                                                                                                                                           ttAACACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCACTGACTGCCACGATg  <  1:594203/249‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     TATCTGCGAAGTGGATGACGAAGAAAGTGAAAATCCGACAGAAAGTGCGGTAGAAAATAACGAAGAATAACAAGATTAGGTTATTTCTATTTTAAGGCAGTAGTGTTTTACTACTGCCTTTACTATTGGAGTGAAAAATGGGTATTTATCTGGTTTATTTGCACATTGTTTGTGCTTTTCTAAGTTTAGGGTTGTTAATTGTGCGTGGGGCAATGCAGTTAATACAGAAAAACTGGCGCGCGGTAAAACTCTTGAAAATCCTACCGCACTTAACGGACACCCTGCTTATTTTGAGCGGTGTCGCCATGTTATTTGTGTTCAACTACGGCTTACCAATTTGGATCATAGTGAAAATTTTACTGCTTGCAGGCTACATTGTCTTCTCGGCAAAATTCTTCAGTAAAAAACAATCTCAAATTCGACCGCACTTTTTCTTCTTCGCCCTTGCGGCACTGACTGCCACGATG  >  1/1757585‑1758051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑