Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,737,612	T→C	S115G (AGT→GGT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,737,612	0	T	C	100.0%	15.1 / NA	7	S115G (AGT→GGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/2);  total (5/2)

CTCTATTATTTTCGAATATGCTCTCATATTTGGATCATTATTAAATTGAATATTATTATAATAGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACTGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATGAGAAAACCGATAAAAGGAATATAACGTTTAATATCCTTAGTTAAGG  >  1/1737487‑1737782                                                                                                                              |                                                                                                                                                                           ctctATTATTTTCGAATATGCTCTCATATTTGGATCATTATTAAATTGAATATTATTATAATAGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATga                                               >  1:173046/1‑251 (MQ=255)                     ctctCATATTTGGATCATTATTAAATTGAATATTATTATAATAGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAAt                                                                                          >  1:712049/1‑188 (MQ=255)                                             aTTGAATATTATTATAATAGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAAcc                                                        >  1:113936/1‑198 (MQ=255)                                              ttGAATATTATTATAATAGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATGAGAAAACCGATAAAAGGAATATAACGTTTAATATCCTTAGTTAAgg  <  1:800534/251‑1 (MQ=255)                                                          ataataGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATGAGAAAACCGATAAAAGGAatat                          <  1:750528/215‑1 (MQ=255)                                                                                                    cATTAAATTAAAGAAAATTATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATGAGAAAACCGATAAAAGGAATATAACGTTTAATATCCTTAGtt      >  1:749183/1‑193 (MQ=255)                                                                                                                     ttATGTTACCGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATGAGAAAAc                                         >  1:668215/1‑141 (MQ=255)                                                                                                                              |                                                                                                                                                                           CTCTATTATTTTCGAATATGCTCTCATATTTGGATCATTATTAAATTGAATATTATTATAATAGACTTTTCCCATTAATAAGAAAACAAATATAAAAAACATTAAATTAAAGAAAATTATGTTACTGTTATTCTTTTTGATTGACCTTTTTTCAAAAATTAAATAATAAAAAGGAAGCATAAAAATGGAAAATCGTTCATATATAAATGCTGTTTGATTAAGTGCATGAGGTAAACTAAACCAAAGAATGAGAAAACCGATAAAAGGAATATAACGTTTAATATCCTTAGTTAAGG  >  1/1737487‑1737782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑