Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,721,456	C→G	intergenic (‑130/+97)	unknown ← / ← unknown	Outer membrane protein A precursor/Excinuclease ABC subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,721,456	0	C	G	100.0%	36.6 / NA	14	intergenic (‑130/+97)	unknown/unknown	Outer membrane protein A precursor/Excinuclease ABC subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/5);  total (9/5)

CCGGCTTTTGCCCCCGCATAAAAGGTATTTGCTTGCGATGCTGCCTGCACAATTGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAACCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTCACTTGATAACATAAAACTTTCCCGTAATTGTTGTAACTGATCGCGCGTTGCAGCAGCTTTCTCAAATTCCAAATCCTGTGCAAATTTATACATTTGCTGCTCAAGTTT  >  1/1721214‑1721666                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                   ccGGCTTTTGCCCCCGCATAAAAGGTATTTGCTTGCGATGCTGCCTGCACAATTGTCGCTACTGATGCAGCTATAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGta                                                                                                                                                                                                            <  1:887712/251‑1 (MQ=255)  cGGCTTTTGCCCCCGCATAAAAGGTATTTGCTTGCGATGCTGCCTGCACAATTGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGtat                                                                                                                                                                                                           <  1:309135/251‑1 (MQ=255)    gCTTTTGCCCCCGCATAAAAGGTATTTGCTTGCGATGCTGCCTGCACAATTGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGta                                                                                                                                                                                                            >  1:699678/1‑248 (MQ=255)                       aGGTATTTGCTTGCGATGCTGCCTGCACAATTGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATcc                                                                                                                                                                                                      <  1:590847/235‑1 (MQ=255)                                                 acaATTGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATCATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATc                                                                                                                                                            <  1:449324/251‑1 (MQ=255)                                                      tGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAatat                                                                                                                                                       >  1:64073/1‑251 (MQ=255)                                                           cTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCa                                                                                                                                                  >  1:524970/1‑251 (MQ=255)                                                                                                                           ccTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATcc                                                                                                                                                                                                      >  1:337228/1‑135 (MQ=255)                                                                                                                                      aaTTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTCACTTGATAACATAAAACTTTCCCGTAATTGTTGTaa                                                                          >  1:552675/1‑248 (MQ=255)                                                                                                                                                               aCTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTCACTTGATAACATAAAACTTTCCCGTAATTGTTGTAACTGATCGCGCGTTGCAGCAGCTTTCTCa                                              <  1:417527/251‑1 (MQ=255)                                                                                                                                                                                 aCTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTCACTTGATAACATAAAACTTTCCCGTAATTGTTGTaa                                                                          >  1:321414/1‑205 (MQ=255)                                                                                                                                                                                          aaaTTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGAATTATcc                                                                                                                                                                                                      >  1:663701/1‑72 (MQ=255)                                                                                                                                                                                                             gggTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTCACTTGATAACATAAAACTTTCCCGTAATTGTTGTAACTGATCGCGCGTTGCAGCAGCTTTCTCAAATTCCAAATCCTGTGCAAATTTATACATTTGCTGCTTAAGttt  >  1:806397/1‑249 (MQ=255)                                                                                                                                                                                                                                             gCTAAAGCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTc                                                                                                              >  1:776879/1‑109 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                   CCGGCTTTTGCCCCCGCATAAAAGGTATTTGCTTGCGATGCTGCCTGCACAATTGTCGCTACTGATGCAGCTAAAGCCGCAGTAGTAGCTAATGCGATTACAGTTTTTTTCATTTTTGCTCTCCTATATTATTAATTTGATTAACTTAAAATATGCTAACTTAAGATTTTAATTTAACTAAAGGCAAATTGAACCGCTTTATTTGGGTAAATTCAGTCCTTTATTTAAAATAAAATGCTAAACCTTTAGTATTATCCTATAAAAAAATAAAAAATCAAATTCCGCTTATTTTTCAGATCAATATAACCAACTTTCCTTTGTTGCTTTTTATATGAAATTTTACTCACTTGATAACATAAAACTTTCCCGTAATTGTTGTAACTGATCGCGCGTTGCAGCAGCTTTCTCAAATTCCAAATCCTGTGCAAATTTATACATTTGCTGCTCAAGTTT  >  1/1721214‑1721666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑