| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,698,090 | C→T | R463R (CGG→CGA) | unknown ← | ATP‑dependent helicase HrpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,698,090 | 0 | C | T | 97.5% | 231.1 / NA | 80 | R463R (CGG→CGA) | unknown | ATP‑dependent helicase HrpA |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (48/30); total (50/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGGCGACCGAGGTGTGTGAGAACACGTTTTTCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGCCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGTTGCACT > 1/1697850‑1698333 | ttGGCGACCGAGGTGTGTGAGAACACGTTTTTCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCTTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCACTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATcc < 1:445562/250‑1 (MQ=255) gtgtgAGAACACGTTTTTCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCTTGAAAACGAGGGGAGCGAAGTTCCTCCTCCTGATAAAATACCCTTTCCCTCCCCCGGTTAAGGGGGGGACTAACGGAAGGCGAAGGGGGCAGATTTGCG‑ATAGGCCTTCCATTCAAACGCCCCCACTCCTTCCAACAATTTCACCCCACCCGGAATATGCCGTTTATCCGGTCCATcaccgaa > 1:444494/1‑244 (MQ=255) gtgAGAACACGTTTTTCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACgg > 1:341354/1‑251 (MQ=255) gaACACGTTTTTCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGaa < 1:637673/244‑1 (MQ=255) ttttCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAat < 1:693197/251‑1 (MQ=255) ttCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAatat < 1:542910/251‑1 (MQ=255) gTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGc < 1:519697/227‑1 (MQ=255) aTTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCg > 1:233624/1‑251 (MQ=255) ttCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAatat < 1:7202/232‑1 (MQ=255) cccccccTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCa > 1:783634/1‑251 (MQ=255) cccccTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCAtt > 1:276967/1‑251 (MQ=255) cccccTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCAtt < 1:458839/251‑1 (MQ=255) ccccTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCAttt > 1:606023/1‑251 (MQ=255) ccTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATcc > 1:340012/1‑194 (MQ=255) ccTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTCCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATcc > 1:607284/1‑194 (MQ=255) gCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAAt > 1:117252/1‑251 (MQ=255) gCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAAt > 1:461430/1‑251 (MQ=255) ggCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGAc < 1:147602/250‑1 (MQ=255) cAGATCCCACTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGc < 1:42004/250‑1 (MQ=255) gATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCaa > 1:384957/1‑251 (MQ=255) ccTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGt > 1:218417/1‑251 (MQ=255) cTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAatat < 1:32710/201‑1 (MQ=255) tGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAAc < 1:498687/184‑1 (MQ=255) gagCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGTGGAAGCACCGAAGGCGGAGGGGGGAGATTTCCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGCCGTTTATCCGGTGCATCCACGAATGGGAAGGCTCCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCg > 1:411094/1‑251 (MQ=255) tCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTaa > 1:888539/1‑251 (MQ=255) tCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTaa > 1:525827/1‑251 (MQ=255) tCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTaa > 1:495214/1‑251 (MQ=255) tctGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCgg > 1:637738/1‑251 (MQ=255) tGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGc < 1:541026/251‑1 (MQ=255) gATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTc > 1:222402/1‑245 (MQ=255) gATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCg > 1:181570/1‑251 (MQ=255) aaaTACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGc < 1:223578/139‑1 (MQ=255) tACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGAttgttg > 1:231214/1‑251 (MQ=255) ttCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCa > 1:39958/1‑135 (MQ=255) ccccccTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTGCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTc < 1:454942/250‑1 (MQ=255) cccccTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAacac < 1:848044/166‑1 (MQ=255) ccccTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTc > 1:738921/1‑225 (MQ=255) ccccTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCg > 1:742171/1‑251 (MQ=255) ccTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGaa > 1:769064/1‑251 (MQ=255) gTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAAc < 1:234460/153‑1 (MQ=255) gTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTc > 1:527926/1‑220 (MQ=255) gTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAAt > 1:563789/1‑249 (MQ=255) gggggAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGa > 1:339053/1‑247 (MQ=255) gAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATAc < 1:344591/250‑1 (MQ=255) aCCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGc < 1:702270/251‑1 (MQ=255) ggggggAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTcaca > 1:210536/1‑250 (MQ=255) ggggggAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTcaca > 1:389411/1‑250 (MQ=255) ggggAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACg > 1:572059/1‑250 (MQ=255) tttGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTcaca > 1:75476/1‑241 (MQ=255) cGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGcc > 1:478454/1‑250 (MQ=255) ccTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGc < 1:709408/251‑1 (MQ=255) ccattccaACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGAt > 1:846611/1‑251 (MQ=255) cattccaACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGAtt > 1:544411/1‑251 (MQ=255) attccaACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAAt < 1:89320/121‑1 (MQ=255) cAATTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGa < 1:591470/187‑1 (MQ=255) aaTTCTTCCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCCACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGa < 1:859258/251‑1 (MQ=255) ttcttcCAACAATTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGaaa < 1:272382/251‑1 (MQ=255) aTTTCACGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATg < 1:359915/251‑1 (MQ=255) aCGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAg < 1:672792/251‑1 (MQ=255) cGCCATCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGa < 1:459919/251‑1 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAAtt > 1:680773/1‑99 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGcc < 1:869039/177‑1 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGc > 1:801723/1‑176 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGc < 1:668928/208‑1 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGc > 1:74919/1‑208 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCgg > 1:646742/1‑218 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATg > 1:307795/1‑241 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATg > 1:165657/1‑241 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGtt > 1:487345/1‑251 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGtt > 1:773503/1‑251 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGtt > 1:520052/1‑251 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGtt > 1:699668/1‑251 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGtt > 1:197247/1‑251 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGtt > 1:64481/1‑251 (MQ=255) aTCCTGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGt > 1:366011/1‑250 (MQ=255) tGAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGTTGCAc < 1:312152/251‑1 (MQ=255) gAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATg > 1:526082/1‑170 (MQ=255) gAATATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGa > 1:201896/1‑239 (MQ=255) aaTATGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGTTGCACt < 1:395317/250‑1 (MQ=255) tatGTCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAgg > 1:4977/1‑213 (MQ=255) | TTGGCGACCGAGGTGTGTGAGAACACGTTTTTCTTTGAAAGTTGGAGTATTCCCCCCCTCAGCCCTACGGGCAGATCCCCCTGTAAACGGAGGGAGCGAAGATCCTTCTTCTGATAAAATACCCTTTCCCTCCCCCTGTTTACGGGGGGAAGTACCGAAGGCGGAGGGGGGAGATTTGCGAATA‑GCCTTCCATTCCAACGCCCCCAATTCTTCCAACAATTTCACGCCATCCTGAATATGCCGTTTATCCGGTGCATCCACGAACGGGAAGGCTTCAATATCATCCAAACCCAACACCGTCATTTGCAAAATCACCGACGCCAAATTGGTTCGCAGGATTTCCGGATCGGTAAATTCCGGGCGATTGTTGAAATCGTCTTCCGAATAAAGACGAATACAAATGCCTTCGCTCACACGCCCGCAACGCCCTTTACGCTGATTGGCGGAGGCTTGTGAAATGGGTTCAATGGGAAGACGTTGCACT > 1/1697850‑1698333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |