Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,697,551	C→T	R643H (CGC→CAC)	unknown ←	ATP‑dependent helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,697,551	0	C	T	100.0%	189.1 / NA	66	R643H (CGC→CAC)	unknown	ATP‑dependent helicase HrpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/30);  total (36/30)

CACCAGCTCCGCCGCCATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGCGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCATAAAC  >  1/1697304‑1697783                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          caccaGCTCCGCCGCCATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGaa                                                                                                                                                                                                                                        <  1:575753/251‑1 (MQ=255) caccaGCTCCGCCGCCATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGaa                                                                                                                                                                                                                                        <  1:288791/251‑1 (MQ=255)       cTCCGCCGCCATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATgcg                                                                                                                                                                                                                                    <  1:508355/249‑1 (MQ=255)            ccgccATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTa                                                                                                                                                                                                                              <  1:898092/250‑1 (MQ=255)               ccATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGACATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTg                                                                                                                                                                                                                          <  1:765391/251‑1 (MQ=255)                     cccATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGaaa                                                                                                                                                                                                                    >  1:717279/1‑251 (MQ=255)                                tgttTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACa                                                                                                                                                                                                         <  1:597519/251‑1 (MQ=255)                                             gCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAAt                                                                                                                                                                                            >  1:420708/1‑251 (MQ=255)                                                  gAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAg                                                                                                                                                                                                                        <  1:105916/218‑1 (MQ=255)                                                  gAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGAc                                                                                                                                                                                                          >  1:710098/1‑232 (MQ=255)                                                  gAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGtt                                                                                                                                                                                       >  1:813862/1‑251 (MQ=255)                                                   aaTTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGtt                                                                                                                                                                                       <  1:520097/250‑1 (MQ=255)                                                          gAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTc                                                                                                                                                                                <  1:786103/250‑1 (MQ=255)                                                                ggCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCtt                                                                                                                                                                         >  1:860430/1‑251 (MQ=255)                                                                     aaTGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTttgt                                                                                                                                                                    <  1:901036/251‑1 (MQ=255)                                                                       tGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTttgttg                                                                                                                                                                  <  1:115762/251‑1 (MQ=255)                                                                       tGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTttgttg                                                                                                                                                                  <  1:487391/251‑1 (MQ=255)                                                                                          gaTATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑Cca                                                                                                                                               >  1:731997/1‑250 (MQ=255)                                                                                          gaTATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑Cca                                                                                                                                               >  1:568986/1‑250 (MQ=255)                                                                                           atatTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑Ccaca                                                                                                                                             >  1:834739/1‑251 (MQ=255)                                                                                           atatTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑Ccaca                                                                                                                                             >  1:691084/1‑251 (MQ=255)                                                                                           atatTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑Ccaca                                                                                                                                             >  1:96550/1‑251 (MQ=255)                                                                                            tatTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAg                                                                                                                                            <  1:496784/251‑1 (MQ=255)                                                                                                gttgttTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGtt                                                                                                                                         >  1:256480/1‑250 (MQ=255)                                                                                                   gttTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAg                                                                                                                                     >  1:503988/1‑251 (MQ=255)                                                                                                    tttttCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGa                                                                                                                                    <  1:473093/251‑1 (MQ=255)                                                                                                     ttttCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGaa                                                                                                                                   <  1:454240/251‑1 (MQ=255)                                                                                                                          atatGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGaa                                                                                                                                                                                                                     <  1:235059/149‑1 (MQ=255)                                                                                                                          atatGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGaa                                                                                                                                   <  1:396291/230‑1 (MQ=255)                                                                                                                          atatGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGAtttttt                                                                                                              >  1:622647/1‑251 (MQ=255)                                                                                                                          atatGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGAtttttt                                                                                                              >  1:178757/1‑251 (MQ=255)                                                                                                                          atatGGGACAACAAGCCACTTAAAAGTGCGGCATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACAGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGAtttttt                                                                                                              >  1:435308/1‑251 (MQ=255)                                                                                                                             tGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTttgttg                                                                                                                                                                  >  1:823697/1‑197 (MQ=255)                                                                                                                                           aCTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAg                                                                                                                                                         <  1:892560/192‑1 (MQ=255)                                                                                                                                           aCTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGAt                                                                                             >  1:87326/1‑251 (MQ=255)                                                                                                                                           aCTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGAt                                                                                             >  1:537604/1‑251 (MQ=255)                                                                                                                                           aCTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTCCACCCACCGCCAGACGAATTTGCTGTTAAATATCCTCCCATTCGTGAATGCGCAGGTATTTGAGGAAACCTTTTTGCCATTGCCGACGGAATTGGTTTTTCCCCAGCGCTTTTTGTTGTTCTTGCAG‑TCATTCCCACAGTTTCAAGAACCCCAAGAAATCCGATTTTTTATCGGCAAAACGACGAt                                                                                             >  1:11841/1‑251 (MQ=255)                                                                                                                                            cTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTc                                                                                                                                                                                >  1:824220/1‑168 (MQ=255)                                                                                                                                            cTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGa                                                                                                                                    >  1:480252/1‑211 (MQ=255)                                                                                                                                                      cGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTc                                                                                   <  1:733523/250‑1 (MQ=255)                                                                                                                                                        gTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGa                                                                                                                                    >  1:104157/1‑199 (MQ=255)                                                                                                                                                                 tGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGACAAAACGACGATGTTTTTCGTCCGCTGCCtgttg                                                                       >  1:403476/1‑251 (MQ=255)                                                                                                                                                                     gATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTc                                                                                                                                                               <  1:381251/160‑1 (MQ=255)                                                                                                                                                                      atatTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGttttt                                                                  >  1:34312/1‑251 (MQ=255)                                                                                                                                                                       tatTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTc                                                                 <  1:76697/251‑1 (MQ=255)                                                                                                                                                                              acaatatctcggcgcgcgcaacgCCCATTTCGCGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGa                                                          >  1:29559/23‑251 (MQ=255)                                                                                                                                                                                          tAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGa                                              >  1:465509/1‑251 (MQ=255)                                                                                                                                                                                                        aTTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAg                                                                                                                                            >  1:50999/1‑143 (MQ=255)                                                                                                                                                                                                              cGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGc                                                                                                                                                                                  >  1:613563/1‑100 (MQ=255)                                                                                                                                                                                                                cACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGaaa                        <  1:479653/251‑1 (MQ=255)                                                                                                                                                                                                                  ccGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACg                      >  1:736797/1‑251 (MQ=255)                                                                                                                                                                                                                  ccGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACg                      >  1:150308/1‑251 (MQ=255)                                                                                                                                                                                                                            aaTTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTTAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCAtt             <  1:380471/250‑1 (MQ=255)                                                                                                                                                                                                                             aTTTGCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTAc           <  1:616788/251‑1 (MQ=255)                                                                                                                                                                                                                                 gCTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGaa                                       >  1:26856/1‑219 (MQ=255)                                                                                                                                                                                                                                  cTGATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCAt      <  1:855495/251‑1 (MQ=255)                                                                                                                                                                                                                                    gATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAGGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGTTCATTCCTTCATaa    >  1:44702/1‑251 (MQ=255)                                                                                                                                                                                                                                    gATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCtgttgt                                                                      <  1:499898/185‑1 (MQ=255)                                                                                                                                                                                                                                    gATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGaa                                       >  1:584597/1‑216 (MQ=255)                                                                                                                                                                                                                                    gATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTAc           >  1:678410/1‑244 (MQ=255)                                                                                                                                                                                                                                    gATAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCATaa    >  1:77948/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGTGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCATaaa   <  1:425947/251‑1 (MQ=255)                                                                                                                                                                                                                                      tAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGaa                                       <  1:24390/214‑1 (MQ=255)                                                                                                                                                                                                                                      tAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCATaaa   >  1:48545/1‑250 (MQ=255)                                                                                                                                                                                                                                      tAAATATCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCATAAAc  <  1:279236/251‑1 (MQ=255)                                                                                                                                                                                                                                         atatCCTGCCATTCGTGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTg                                         <  1:890743/209‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          CACCAGCTCCGCCGCCATTACCCATTTCGGTTGTTTTTTGAAAAGCACGGAATTGGGGAAAATGGCGAAATGGGCGTTGCGCGCGCCGAGATATTGTTGTTTTTCCGCTTCTTTCAGCCCGATATGGGACAACAAGCCACTTAAAAGTGCGGTATGAATTTGCTGATATTCGGCTTTTTCGGAATTAATCGGCAAGCCCATTTCACGCACCGCCAGACGAATTTGCTGATAAATATCCTGCCATTCGCGAATGCGCAGGTAATTGAGGAAATCTTTTTGACATTGGCGACGGAATTGGTTTTTGCTCAGCGCTTTTTGTTGTTCTTGCAGATAATT‑CCACAGGTTCAAGAACGCCAAGAAATCCGATTTTTTATCGGCAAAACGACGATGTTTTTCGTCCGCTGCCTGTTGTTTTTCCGTCGGACGTTCGCGCAGATCCTGAATGGATAACGCCGAAACGATGATCATTACTTCATAAAC  >  1/1697304‑1697783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑