Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,643,250	C→T	G660G (GGC→GGT)	unknown →	Alpha‑glucosidase (EC 3.2.1.20)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,643,250	0	C	T	100.0%	174.2 / NA	58	G660G (GGC→GGT)	unknown	Alpha‑glucosidase (EC 3.2.1.20)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

TTATTTATACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGCGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCAT  >  1/1643016‑1643481                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        ttatttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACgg                                                                                                                                                                                                                         >  1:523762/1‑251 (MQ=255)  tatttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGt                                                                                                                                                                                                                        >  1:334450/1‑251 (MQ=255)  tatttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGt                                                                                                                                                                                                                        <  1:730354/251‑1 (MQ=255)  tatttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGt                                                                                                                                                                                                                        >  1:721059/1‑251 (MQ=255)   atttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTa                                                                                                                                                                                                                       >  1:30232/1‑251 (MQ=255)   atttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTa                                                                                                                                                                                                                       >  1:173554/1‑251 (MQ=255)   atttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTa                                                                                                                                                                                                                       >  1:216170/1‑251 (MQ=255)   atttatACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGCGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTa                                                                                                                                                                                                                       >  1:289299/1‑251 (MQ=255)                  tttGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGaa                                                                                                                                                                                                        <  1:576328/251‑1 (MQ=255)                   ttGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGaaa                                                                                                                                                                                                       >  1:884584/1‑251 (MQ=255)                      gAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAgg                                                                                                                                                                                                     <  1:297128/250‑1 (MQ=255)                        aaGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATc                                                                                                                                                                                                  <  1:717835/251‑1 (MQ=255)                                        cGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAagag                                                                                                                                                                                  >  1:329501/1‑251 (MQ=255)                                         gAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAagag                                                                                                                                                                                  >  1:1217/1‑250 (MQ=255)                                          aaCCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGcc                                                                                                                                                                                                               >  1:146039/1‑220 (MQ=255)                                            ccTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGaa                                                                                                                                                                                                        <  1:312282/225‑1 (MQ=255)                                              tataTTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGaa                                                                                                                                                                            <  1:80330/251‑1 (MQ=255)                                                             tttCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATgg                                                                                                                                                             <  1:91628/251‑1 (MQ=255)                                                              ttCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTc                                                                                                                                                                   >  1:556492/1‑244 (MQ=255)                                                                     aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATa                                                                                                                                                                                                                   <  1:885626/189‑1 (MQ=255)                                                                     aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaaa                                                                                                                                                     >  1:227299/1‑251 (MQ=255)                                                                     aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaa                                                                                                                                                      <  1:863613/250‑1 (MQ=255)                                                                     aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAACTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaa                                                                                                                                                      <  1:409313/250‑1 (MQ=255)                                                                              aTGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATaaa                                                                                                                                            <  1:101042/251‑1 (MQ=255)                                                                               tGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCCCgg                                                                                                                                                                                                                         >  1:434112/1‑173 (MQ=255)                                                                               tGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAag                                                                                                                                                                                    >  1:113948/1‑210 (MQ=255)                                                                                gATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATaaaaa                                                                                                                                          >  1:258257/1‑251 (MQ=255)                                                                                   aaaaaCACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATAc                                                                                                                                       <  1:424429/251‑1 (MQ=255)                                                                                     aaaCACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACAt                                                                                                                                     <  1:638070/251‑1 (MQ=255)                                                                                         acaTTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATc                                                                                                                                 <  1:772692/251‑1 (MQ=255)                                                                                         acaTTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATc                                                                                                                                 <  1:383631/251‑1 (MQ=255)                                                                                          caTTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAg                                                                                                                                                                           >  1:406713/1‑208 (MQ=255)                                                                                                cAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCtgatga                                                                                                                           <  1:389906/250‑1 (MQ=255)                                                                                                        gatgatTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCgaga                                                                                                                  >  1:734413/1‑251 (MQ=255)                                                                                                            atTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaa                                                                                                                                                      <  1:140164/211‑1 (MQ=255)                                                                                                                        gTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGcc                                                                                                   <  1:759786/250‑1 (MQ=255)                                                                                                                             gATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTAt                                                                                                                                  <  1:656139/214‑1 (MQ=255)                                                                                                                                          cATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGagaa                                                                                >  1:771063/1‑251 (MQ=255)                                                                                                                                          cATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGagaa                                                                                <  1:254421/251‑1 (MQ=255)                                                                                                                                               gtgGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACAt                                                                                                                                     <  1:150435/193‑1 (MQ=255)                                                                                                                                               gtgGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCtgatga                                                                                                                           >  1:620599/1‑203 (MQ=255)                                                                                                                                                      aaCCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAg                                                                                                                                                                           <  1:437119/148‑1 (MQ=255)                                                                                                                                                            aTCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGttt                                                              >  1:647907/1‑251 (MQ=255)                                                                                                                                                               aaaGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTtgat                                                           <  1:522552/251‑1 (MQ=255)                                                                                                                                                                aaGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTtgatg                                                          <  1:776713/251‑1 (MQ=255)                                                                                                                                                                aaGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGAGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTG‑Ttgatga                                                         <  1:483844/251‑1 (MQ=255)                                                                                                                                                                  gagTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACAt                                                                                                                                     >  1:78019/1‑174 (MQ=255)                                                                                                                                                                  gagTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGagaaaga                                                                             >  1:742549/1‑230 (MQ=255)                                                                                                                                                                   agTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTtgatga                                                         <  1:102440/249‑1 (MQ=255)                                                                                                                                                                         ggATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAattatt                                                                                                            <  1:504267/192‑1 (MQ=255)                                                                                                                                                                         ggATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATc                                                                                        >  1:105814/1‑212 (MQ=255)                                                                                                                                                                                                         ggTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAg                                                                   >  1:900137/1‑201 (MQ=255)                                                                                                                                                                                                         ggTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAata                     <  1:475715/247‑1 (MQ=255)                                                                                                                                                                                                                cTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGttt                                          <  1:855142/219‑1 (MQ=255)                                                                                                                                                                                                                cTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCtt           >  1:861545/1‑250 (MQ=255)                                                                                                                                                                                                                     acacTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATc                                                                                        >  1:340541/1‑168 (MQ=255)                                                                                                                                                                                                                          cATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCAt  >  1:56724/1‑249 (MQ=255)                                                                                                                                                                                                                                  gTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAattatt                                                                                                            >  1:558056/1‑135 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        TTATTTATACAATACACTTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGCGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCAT  >  1/1643016‑1643481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑