Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,628,842	T→G	A60A (GCT→GCG)	unknown →	Nucleoside 5‑triphosphatase RdgB (dHAPTP, dITP, XTP‑specific) (EC 3.6.1.66)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,628,842	0	T	G	100.0%	213.8 / NA	75	A60A (GCT→GCG)	unknown	Nucleoside 5‑triphosphatase RdgB (dHAPTP, dITP, XTP‑specific) (EC 3.6.1.66)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/34);  total (41/34)

AAAAAAATCGCGACCGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCTCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGAAAAATCGCGCCAAATTATTGGTGGAATTAGCCCATGTGCCGG  >  1/1628609‑1629083                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  aaaaaaaTCGCGACCGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGt                                                                                                                                                                                                                                    <  1:613733/249‑1 (MQ=255) aaaaaaaTCGCGACCGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGGCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGt                                                                                                                                                                                                                                    <  1:451037/249‑1 (MQ=255)               cGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGc                                                                                                                                                                                                                           >  1:393099/1‑244 (MQ=255)               cGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGgaag                                                                                                                                                                                                                    >  1:351054/1‑251 (MQ=255)               cGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGgaag                                                                                                                                                                                                                    <  1:865862/251‑1 (MQ=255)                       cTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACgggg                                                                                                                                                                                                            >  1:732105/1‑251 (MQ=255)                        ttATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGTAGCAAAACATTGTATTGCCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGCTTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGAATTGACAGTCCGGAAGAAACgggg                                                                                                                                                                                                            >  1:44237/1‑250 (MQ=255)                             aTCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGAcg                                                                                                                                                                                                      >  1:75929/1‑251 (MQ=255)                                      cTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGAcgtt                                                                                                                                                                                                    <  1:781115/244‑1 (MQ=255)                                                 gttTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGTCAtttt                                                                                                                                                                                  >  1:41581/1‑251 (MQ=255)                                                 gttTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCAtttt                                                                                                                                                                                  >  1:404666/1‑251 (MQ=255)                                                      aaTGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCACTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAg                                                                                                                                                                             <  1:717947/251‑1 (MQ=255)                                                       aTGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGt                                                                                                                                                                                                                                    <  1:723397/195‑1 (MQ=255)                                                                  ggCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGaa                                                                                                                                                                                             >  1:646562/1‑223 (MQ=255)                                                                   gCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATgc                                                                                                                                                                   <  1:89898/248‑1 (MQ=255)                                                                        gTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGaaa                                                                                                                                                           >  1:412274/1‑251 (MQ=255)                                                                               gTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATgc                                                                                                                                                                   <  1:574340/236‑1 (MQ=255)                                                                                   tttttATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACgggg                                                                                                                                                                                                            >  1:547966/1‑191 (MQ=255)                                                                                   tttttATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTa                                                                                                                                                >  1:857326/1‑251 (MQ=255)                                                                                   tttttATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTa                                                                                                                                                >  1:175872/1‑251 (MQ=255)                                                                                                cAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcg                                                                                                                                   >  1:517653/1‑251 (MQ=255)                                                                                                cAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcg                                                                                                                                   >  1:733353/1‑251 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGt                                                                                                                                                                                                                                    >  1:600206/1‑152 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGt                                                                                                                                                                                                                                    <  1:840763/152‑1 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTg                                                                                                                                                                                               <  1:568016/189‑1 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAg                                                                                                                                                                             <  1:824671/207‑1 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCa                                                                                                                                         >  1:218238/1‑243 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:773486/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:768435/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:857042/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:511650/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:789062/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:387575/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:792235/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:70554/1‑250 (MQ=255)                                                                                                  gTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcga                                                                                                                                  >  1:669466/1‑250 (MQ=255)                                                                                                      taataCGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGAc                                                                                                                                                                                                                              >  1:134150/1‑154 (MQ=255)                                                                                                             gggCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGtt                                                                                                                      >  1:131136/1‑251 (MQ=255)                                                                                                             gggCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGt                                                                                                                       <  1:299327/250‑1 (MQ=255)                                                                                                                  tttttCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCggg                                                                                                                        <  1:267149/244‑1 (MQ=255)                                                                                                                  tttttCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGt                                                                                                                       >  1:233327/1‑245 (MQ=255)                                                                                                                  tttttCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTtggtgg                                                                                                                 <  1:279916/251‑1 (MQ=255)                                                                                                                  tttttCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTtggtgg                                                                                                                 <  1:46400/251‑1 (MQ=255)                                                                                                                            gCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACATGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTggtggt                                                                                                                >  1:211029/1‑242 (MQ=255)                                                                                                                             cACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTg                                                                                                      >  1:684320/1‑251 (MQ=255)                                                                                                                                ttGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAAc                                                                                                   <  1:223981/251‑1 (MQ=255)                                                                                                                                 tGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACg                                                                                                  >  1:849703/1‑251 (MQ=255)                                                                                                                                      gaACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGgcgcg                                                                                             >  1:631082/1‑251 (MQ=255)                                                                                                                                          aaacaTGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCgg                                                                                         >  1:372810/1‑251 (MQ=255)                                                                                                                                            acaTGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCgggg                                                                                       <  1:121948/251‑1 (MQ=255)                                                                                                                                             caTGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGt                                                                                      <  1:606649/251‑1 (MQ=255)                                                                                                                                             caTGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGt                                                                                      >  1:474738/1‑251 (MQ=255)                                                                                                                                                    cAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTatat                                                                                 <  1:655501/249‑1 (MQ=255)                                                                                                                                                    cAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTatat                                                                                 <  1:397696/249‑1 (MQ=255)                                                                                                                                                    cAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTatat                                                                                 <  1:23212/249‑1 (MQ=255)                                                                                                                                                    cAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTatat                                                                                 <  1:244114/249‑1 (MQ=255)                                                                                                                                                    cAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTc                                                                               <  1:735779/251‑1 (MQ=255)                                                                                                                                                    cAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTc                                                                               <  1:316865/251‑1 (MQ=255)                                                                                                                                                     aaaaaaTTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAgcg                                                                                                                                                                           <  1:345455/158‑1 (MQ=255)                                                                                                                                                            tGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCt                                                                       <  1:89560/251‑1 (MQ=255)                                                                                                                                                               aTTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATg                                                                    >  1:544486/1‑251 (MQ=255)                                                                                                                                                                  ggCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTggtggt                                                                                                                >  1:374054/1‑204 (MQ=255)                                                                                                                                                                    cGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCgg                                                                <  1:473370/250‑1 (MQ=255)                                                                                                                                                                    cGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCgg                                                                <  1:611433/250‑1 (MQ=255)                                                                                                                                                                           cAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCgggg                                                                                       <  1:691075/220‑1 (MQ=255)                                                                                                                                                                              ccAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTatat                                                                                 <  1:683442/223‑1 (MQ=255)                                                                                                                                                                                  ggcaaggTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACgg                                                                                                 >  1:817396/1‑203 (MQ=255)                                                                                                                                                                                    caaggTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGAcg                                               <  1:813018/251‑1 (MQ=255)                                                                                                                                                                                       ggTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCcgac                                                                                                                                 <  1:285327/166‑1 (MQ=255)                                                                                                                                                                                       ggTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGa                                            >  1:13062/1‑251 (MQ=255)                                                                                                                                                                                                 aTGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTatat                                                                                 >  1:8415/1‑204 (MQ=255)                                                                                                                                                                                                                       tCGGTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGAAAAATCGCGCCAAATTATTGGCGGAATTAGcc            <  1:780107/251‑1 (MQ=255)                                                                                                                                                                                                                          gTTTTGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGAAAAATCGCGCCAAATTATTGGCGGAATTAGCCCAt         >  1:720459/1‑251 (MQ=255)                                                                                                                                                                                                                              tGACGTGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGAAAAATCGCGCCAAATTATTGGCGGAATTAGCCCATGTGc     <  1:289273/251‑1 (MQ=255)                                                                                                                                                                                                                                   tGATTGCGCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGAAAAATCGCGCCAAATTATTGGCGGAATTAGCCCATGTGCCgg  >  1:739830/1‑249 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  AAAAAAATCGCGACCGTATCGCCTTATAATCTTTTCCCTTTAGTTGTTGTTTTAATGCAAGACGAGGCAATGTTCCTCGTCTTTTTTATTGGGCGCAGTTATAATACGGGGCATTTTTCAACCGCACTTGAGAGAACAAACATGAAGCAAAAAATTGTATTGGCGACCGGCAACCAAGGCAAGGTCAGAGAAATGGCGGATGTGTTGGCGGATTTCGGTTTTGACGTGATTGCTCAGACGGATTTGGGTATCGACAGCCCGGAAGAAACGGGGCTGACGTTCGTTGAAAATGCCATTTTAAAAGCGCGTTATGCCGCCGAAAAATCCGGTTTACCTGCCATTGCCGACGATTCCGGGTTGGTGGTTGACGCGTTGAACGGCGCGCCGGGGTTATATTCCGCACGCTATGCCGGCGAAGAGGGCAATGACGCGAAAAATCGCGCCAAATTATTGGTGGAATTAGCCCATGTGCCGG  >  1/1628609‑1629083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑