Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,624,653	A→G	P308P (CCA→CCG)	unknown →	Threonine synthase (EC 4.2.3.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,624,653	0	A	G	100.0%	225.0 / NA	79	P308P (CCA→CCG)	unknown	Threonine synthase (EC 4.2.3.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/46);  total (33/46)

CGGCGGCGCAATTACCGAAAAAACAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCACGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCAGCGAAATTCAAGGAATCTGTTGAGCGC  >  1/1624409‑1624887                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           cggcggcgCAATTACCGAAAAAACAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGtgtg                                                                                                                                                                                                                                      >  1:545174/1‑251 (MQ=255)      gcgcAATTACCGAAAAAACAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGgaaga                                                                                                                                                                                                                                 >  1:411304/1‑251 (MQ=255)      gcgcAATTACCGAAAAAACAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGgaag                                                                                                                                                                                                                                  <  1:489711/250‑1 (MQ=255)         cAATTACCGAAAAAACAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAAt                                                                                                                                                                                                                               <  1:782486/250‑1 (MQ=255)                  aaaaaaCAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACg                                                                                                                                                                                                                     <  1:368605/251‑1 (MQ=255)                  aaaaaaCAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACg                                                                                                                                                                                                                     <  1:860804/251‑1 (MQ=255)                   aaaaaCAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGt                                                                                                                                                                                                                    >  1:635636/1‑251 (MQ=255)                           cGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGc                                                                                                                                                                                                              <  1:419968/249‑1 (MQ=255)                                    ctcGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTaa                                                                                                                                                                                                    >  1:160169/1‑250 (MQ=255)                                    ctcGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTaa                                                                                                                                                                                                    >  1:83508/1‑250 (MQ=255)                                    ctcGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAAc                                                                                                                                                                                                   <  1:632363/251‑1 (MQ=255)                                            ctcCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTa                                                                                                                                                                                            >  1:680393/1‑250 (MQ=255)                                            ctcCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTa                                                                                                                                                                                            >  1:494175/1‑250 (MQ=255)                                            ctcCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTAc                                                                                                                                                                                           <  1:297917/251‑1 (MQ=255)                                              ccGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACAt                                                                                                                                                                                         <  1:461008/251‑1 (MQ=255)                                              ccGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACAt                                                                                                                                                                                         <  1:498948/251‑1 (MQ=255)                                                gTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACAt                                                                                                                                                                                         <  1:244682/249‑1 (MQ=255)                                                            aaCTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCAttt                                                                                                                                                                            >  1:184900/1‑250 (MQ=255)                                                                             cgccgGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGgaag                                                                                                                                                                                                                                  >  1:856785/1‑179 (MQ=255)                                                                                  gCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAAc                                                                                                                                                      <  1:527787/250‑1 (MQ=255)                                                                                         cGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTAcgcg                                                                                                                                              >  1:366530/1‑251 (MQ=255)                                                                                           ccAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTAcgcg                                                                                                                                              >  1:872624/1‑249 (MQ=255)                                                                                                 cccTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACg                                                                                                                                                                                                                     >  1:143121/1‑172 (MQ=255)                                                                                                       ttgatagcgacggtgcgaatattgccgccACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACt                                                                                                                                 <  1:568916/227‑1 (MQ=255)                                                                                                               aTCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGttattt                                                                                                                        <  1:539067/251‑1 (MQ=255)                                                                                                                tCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGttattta                                                                                                                       <  1:678332/251‑1 (MQ=255)                                                                                                                    aCGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCg                                                                                                                   <  1:95361/251‑1 (MQ=255)                                                                                                                     cGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACAt                                                                                                                                                                                                       <  1:542630/166‑1 (MQ=255)                                                                                                                                  ccaccaATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACgcgc                                                                                                                                             >  1:57891/1‑211 (MQ=255)                                                                                                                                  ccaccaATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCg                                                                                                                   <  1:271297/237‑1 (MQ=255)                                                                                                                                      caATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTgg                                                                                                 <  1:771742/251‑1 (MQ=255)                                                                                                                                          gCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACgcgc                                                                                                                                             >  1:395282/1‑203 (MQ=255)                                                                                                                                             aaCGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGa                                                                                          <  1:146672/251‑1 (MQ=255)                                                                                                                                             aaCGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGa                                                                                          <  1:112114/251‑1 (MQ=255)                                                                                                                                               cGACACCGTGCCGCGCTATTTACACAGTGGCACCTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAgg                                                                                        <  1:421459/251‑1 (MQ=255)                                                                                                                                               cGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAgg                                                                                        <  1:136794/251‑1 (MQ=255)                                                                                                                                                 acacCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATggg                                                                                                                                                                    <  1:413146/173‑1 (MQ=255)                                                                                                                                                 acacCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAAc                                                                                                                                                      >  1:870932/1‑187 (MQ=255)                                                                                                                                                         ccGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAAc                                                                               <  1:259582/250‑1 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGc                                                                                                                                        <  1:337316/188‑1 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGgcgc                                                                                                      <  1:11938/222‑1 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGgc                                                                                                        >  1:170714/1‑220 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGa                                                                                          >  1:235232/1‑234 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAgg                                                                                        <  1:456753/236‑1 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGAtt                                                                         >  1:85270/1‑251 (MQ=255)                                                                                                                                                              cTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTGATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGAtt                                                                         >  1:848383/1‑251 (MQ=255)                                                                                                                                                               tATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAAc                                                                               <  1:37458/244‑1 (MQ=255)                                                                                                                                                                aTTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACAt                                                                                                                                                    >  1:227127/1‑174 (MQ=255)                                                                                                                                                                aTTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAgg                                                                                        <  1:867603/234‑1 (MQ=255)                                                                                                                                                                aTTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTa                                                                       >  1:745766/1‑251 (MQ=255)                                                                                                                                                                aTTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTCCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTa                                                                                                                                                  <  1:406646/176‑1 (MQ=255)                                                                                                                                                                        aGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACggg                                                               >  1:61021/1‑251 (MQ=255)                                                                                                                                                                         gTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAAc                                                                                                                                                      >  1:133026/1‑163 (MQ=255)                                                                                                                                                                         gTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACt                                                                                                                                 <  1:397143/184‑1 (MQ=255)                                                                                                                                                                         gTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCg                                                                                                                   <  1:125005/198‑1 (MQ=255)                                                                                                                                                                         gTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACgggg                                                              >  1:436025/1‑250 (MQ=255)                                                                                                                                                                         gTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGt                                                              >  1:519749/1‑251 (MQ=255)                                                                                                                                                                                  ggTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGt                                                     >  1:229957/1‑251 (MQ=255)                                                                                                                                                                                          aaCGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTcc                                              <  1:653007/250‑1 (MQ=255)                                                                                                                                                                                          aaCGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTcc                                              <  1:876412/250‑1 (MQ=255)                                                                                                                                                                                          aaCGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTcc                                              <  1:59448/250‑1 (MQ=255)                                                                                                                                                                                          aaCGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTcc                                              <  1:192357/250‑1 (MQ=255)                                                                                                                                                                                              ccACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCg                                                                                                                   <  1:155934/177‑1 (MQ=255)                                                                                                                                                                                              ccACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTaaa                                                                                <  1:67010/212‑1 (MQ=255)                                                                                                                                                                                                    ttGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAAc                                                                               >  1:390888/1‑207 (MQ=255)                                                                                                                                                                                                        cACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGcccacc                               <  1:790980/251‑1 (MQ=255)                                                                                                                                                                                                         ccccTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGcccaccc                              <  1:601600/250‑1 (MQ=255)                                                                                                                                                                                                          cccTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCACACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGcccacccg                             >  1:453686/1‑250 (MQ=255)                                                                                                                                                                                                            cTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGcccacccgg                            >  1:281671/1‑248 (MQ=255)                                                                                                                                                                                                              ttCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGcaca                                                                                                                                     >  1:885409/1‑143 (MQ=255)                                                                                                                                                                                                              ttCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGCGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTc                                               >  1:884882/1‑229 (MQ=255)                                                                                                                                                                                                                        aTGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCGGCGAAATTCAAGGa               <  1:555292/251‑1 (MQ=255)                                                                                                                                                                                                                        aTGGACGTTTCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCGGCGAAATTCAAGGa               <  1:44526/251‑1 (MQ=255)                                                                                                                                                                                                                               tttCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATg                                                                                           >  1:806810/1‑168 (MQ=255)                                                                                                                                                                                                                               tttCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCGGCGAAATTCAAgg                <  1:598713/243‑1 (MQ=255)                                                                                                                                                                                                                               tttCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCGGCGAAATTCAAGGAATCTGt         <  1:21433/250‑1 (MQ=255)                                                                                                                                                                                                                                ttCCCGCCCGAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCGGCGAAATTCAAGGAATCTGTTg       <  1:412572/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccccgAATAACTGGCCGCGTGTGGAAGAAGTGTTCAAGCGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAACCCGGTTTATTCCTTTGCACCGCCCACCCGGCGAAATTCAAGGAATCTGTTGAgcgc  <  1:186234/250‑1 (MQ=255)                                                                                                                                                                                                                                      cccgAATAACTGGCCGCGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCAc                                                                                                           >  1:128902/1‑145 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           CGGCGGCGCAATTACCGAAAAAACAGCGTGAAAATCTCGTAGTCTCCGTGCCAAGCGGCAACTTCGGCAACCTCACCGCCGGCTTAATCGCCAAAACCCTTGGCTTGCCGATCAAACGCTTTATCGCCGCCACCAATGCAAACGACACCGTGCCGCGCTATTTACACAGTGGCAACTGGTCACCGAACGCCACCGTTGCCACCCTTTCCAACGCCATGGACGTTTCCCGCCCGAATAACTGGCCACGTGTGGAAGAATTGTTCAAACGTAACGGCTGGACATTAACCGAATTACATTCCGCCGCCATTTCCGATGGGCAAACGGAAGAAACATTACGCGCAATGCACAAACTCGGTTATTTATGCGAGCCGCACGGCGCGGTGGCGTATGAGGTGCTTAAACAGGATTTACAAACGGGTGAAACCGGTTTATTCCTTTGCACCGCCCACCCAGCGAAATTCAAGGAATCTGTTGAGCGC  >  1/1624409‑1624887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑