BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,617,023	A→G	Q182Q (CAA→CAG)	unknown →	Glycine betaine ABC transport system, ATP‑binding protein OpuAA (EC 3.6.3.32)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,617,023	0	A	G	100.0%	185.7 / NA	61	Q182Q (CAA→CAG)	unknown	Glycine betaine ABC transport system, ATP‑binding protein OpuAA (EC 3.6.3.32)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/30); total (31/30)

ATTCTTGGGTGGCACAAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAAGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATGCAATATGAG  >  1/1616802‑1617257

atTCTTGGGTGGCACAAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAAtt                                                                                                                                                                                                               >  1:312490/1‑251 (MQ=255)

atTCTTGGGTGGCACAAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAAtt                                                                                                                                                                                                               >  1:511945/1‑251 (MQ=255)

 ttCTTGGGTGGCACAAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAAtt                                                                                                                                                                                                               >  1:619935/1‑250 (MQ=255)

             acaAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAG‑ACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCg                                                                                                                                                                                                 <  1:147079/251‑1 (MQ=255)

              caAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCg                                                                                                                                                                                                 <  1:385643/251‑1 (MQ=255)

                              tCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAAttt                                                                                                                                                                                                                                  >  1:556438/1‑202 (MQ=255)

                                     tgAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATcc                                                                                                                                                                                                                         >  1:490557/1‑204 (MQ=255)

                                      gAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACt                                                                                                                                                                                      <  1:271813/238‑1 (MQ=255)

                                          aCATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCaagaag                                                                                                                                                                      >  1:757494/1‑250 (MQ=255)

                                               aCTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTaa                                                                                                                                                                >  1:292776/1‑251 (MQ=255)

                                               aCTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTaa                                                                                                                                                                >  1:399911/1‑251 (MQ=255)

                                               aCTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTaa                                                                                                                                                                >  1:439834/1‑251 (MQ=255)

                                                  tGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAAc                                                                                                                                                             <  1:589918/251‑1 (MQ=255)

                                                      ttAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCaag                                                                                                                                                                         >  1:729221/1‑235 (MQ=255)

                                                          tGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGt                                                                                                                                                     <  1:135103/251‑1 (MQ=255)

                                                               ttGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCAt                                                                                                                                                <  1:250609/251‑1 (MQ=255)

                                                                  ccACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTgg                                                                                                                                             <  1:122930/251‑1 (MQ=255)

                                                                     ccTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGt                                                                                                                                          <  1:240442/251‑1 (MQ=255)

                                                                        gcgcAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCAt                                                                                                                                       >  1:383872/1‑251 (MQ=255)

                                                                             atatCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGa                                                                                                                                  >  1:68566/1‑251 (MQ=255)

                                                                             atatCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGa                                                                                                                                  >  1:757107/1‑251 (MQ=255)

                                                                             atatCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGa                                                                                                                                  >  1:878502/1‑251 (MQ=255)

                                                                               atCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAAt                                                                                                                                <  1:40096/251‑1 (MQ=255)

                                                                                 cAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATgg                                                                                                                              >  1:139614/1‑251 (MQ=255)

                                                                                    gATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGAAAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAAt                                                                                                                                >  1:738661/1‑246 (MQ=255)

                                                                                      tCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGc                                                                                                                         <  1:740979/251‑1 (MQ=255)

                                                                                                cgcgAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGt                                                                                                               <  1:594728/251‑1 (MQ=255)

                                                                                                  cgAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAg                                                                                                             >  1:523657/1‑251 (MQ=255)

                                                                                                    aaCTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGt                                                                                                           >  1:137718/1‑251 (MQ=255)

                                                                                                      cTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTac                                                                                                         <  1:598779/251‑1 (MQ=255)

                                                                                                      cTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTac                                                                                                         <  1:128774/251‑1 (MQ=255)

                                                                                                                     caacaacGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCa                                                                                          >  1:5214/1‑251 (MQ=255)

                                                                                                                        caacGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATAGGAAGCTGGTACAAGTa                                                                                                              >  1:280634/1‑228 (MQ=255)

                                                                                                                         aacGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCaa                                                                                      <  1:592942/251‑1 (MQ=255)

                                                                                                                             gATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAg                                                                                   >  1:42620/1‑250 (MQ=255)

                                                                                                                                       ttACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCACTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTac                                                                                                         <  1:455617/218‑1 (MQ=255)

                                                                                                                                        tACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGt                                                                       <  1:669015/251‑1 (MQ=255)

                                                                                                                                           gggCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACACATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTa                                                                     >  1:847507/1‑250 (MQ=255)

                                                                                                                                                      gctAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCg                                                           >  1:27855/1‑249 (MQ=255)

                                                                                                                                                                  ttATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAAt                                                             >  1:232318/1‑235 (MQ=255)

                                                                                                                                                                       gCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGaa                                                                                                                                 <  1:150107/162‑1 (MQ=255)

                                                                                                                                                                        cTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAAc                                        <  1:56619/250‑1 (MQ=255)

                                                                                                                                                                                  aaCCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAAc                              <  1:251866/250‑1 (MQ=255)

                                                                                                                                                                                    ccATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGt                           >  1:144199/1‑251 (MQ=255)

                                                                                                                                                                                    ccATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGt                           >  1:872231/1‑251 (MQ=255)

                                                                                                                                                                                            tGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAg                   <  1:396785/251‑1 (MQ=255)

                                                                                                                                                                                            tGCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAg                   <  1:315456/251‑1 (MQ=255)

                                                                                                                                                                                             gCACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGt                  >  1:484816/1‑251 (MQ=255)

                                                                                                                                                                                              cACTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGt                  <  1:679574/250‑1 (MQ=255)

                                                                                                                                                                                                cTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGt                                                                                                               <  1:42466/155‑1 (MQ=255)

                                                                                                                                                                                                cTTGATCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGAt               <  1:776346/251‑1 (MQ=255)

                                                                                                                                                                                                    aTCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAAc                              >  1:507251/1‑232 (MQ=255)

                                                                                                                                                                                                    aTCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATg           >  1:553151/1‑251 (MQ=255)

                                                                                                                                                                                                     tCCACTGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATGc          <  1:414644/251‑1 (MQ=255)

                                                                                                                                                                                                          tGGTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATGCAatat     <  1:654033/251‑1 (MQ=255)

                                                                                                                                                                                                           ggTGCGGGCTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTc                                             <  1:514422/210‑1 (MQ=255)

                                                                                                                                                                                                                   cTGCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATGCAATATGAg  <  1:255151/245‑1 (MQ=255)

                                                                                                                                                                                                                     gCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTTAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAAc                                        <  1:528661/205‑1 (MQ=255)

                                                                                                                                                                                                                     gCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGt                                                                                                           >  1:490406/1‑138 (MQ=255)

                                                                                                                                                                                                                     gCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGt                           >  1:746477/1‑218 (MQ=255)

                                                                                                                                                                                                                     gCCTTACAGGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATGCAatat     <  1:774960/240‑1 (MQ=255)

ATTCTTGGGTGGCACAAAAATGAGCGAAAATCCCGTGTGAATACATTACTTGAATTAGTGAATTTGCCACCTGCGCAATATCAGGATCGCTATCCGCGCGAACTTTCCGGTGGAGAACAACAACGGATAGGCATTTTACGGGCTATTGCTGCTAATCCCCATTTATTGCTCATGGATGAACCATTTTCTGCACTTGATCCACTGGTGCGGGCTGCCTTACAAGATCAAATTTCGTTGATCCATAAAAAATTCGGCACAACTATCGTGTTCGTTACTCATGACATGCAAGAAGCCTTAAAACTTGCTTGTCGCATTGGCGTCATGCAGAATGGGAAGCTGGTACAAGTAGGTACACCAAACGAAATCCAGCAACAGCCTGCCAATAGTTACGTAAAATCGCTATTCGCTTCGTCTGAACAGGCGTTAACGGTAGAGAAAGTGATTATGCAATATGAG  >  1/1616802‑1617257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`