Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,613,165	A→G	T217A (ACC→GCC)	unknown →	4‑hydroxy‑tetrahydrodipicolinate synthase (EC 4.3.3.7)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,613,165	0	A	G	100.0%	189.0 / NA	67	T217A (ACC→GCC)	unknown	4‑hydroxy‑tetrahydrodipicolinate synthase (EC 4.3.3.7)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/31);  total (36/31)

ATGTACCGGGACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCACCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGATGCATTAAAAGCCGCCGGCTTGCTT  >  1/1612932‑1613407                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   aTGTACCGGGACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAAtg                                                                                                                                                                                                                                   >  1:344756/1‑251 (MQ=255)   gTACCGGGACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCaaaaa                                                                                                                                                                                                                                     <  1:403793/247‑1 (MQ=255)   gTACCGGGACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAAtgtg                                                                                                                                                                                                                                 >  1:91564/1‑251 (MQ=255)        gggACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCAt                                                                                                                                                                                                                             >  1:502731/1‑250 (MQ=255)         ggACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCAtc                                                                                                                                                                                                                            <  1:111754/250‑1 (MQ=255)         ggACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCAt                                                                                                                                                                                                                             >  1:620001/1‑249 (MQ=255)          gACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCAtct                                                                                                                                                                                                                           <  1:88734/250‑1 (MQ=255)              cACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTGCAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGcc                                                                                                                                                                                                                       <  1:720090/250‑1 (MQ=255)              cACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGcc                                                                                                                                                                                                                       <  1:444022/250‑1 (MQ=255)                        gACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGata                                                                                                                                                                                                                                              <  1:21344/217‑1 (MQ=255)                           ttATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAAtt                                                                                                                                                                                                         <  1:808205/251‑1 (MQ=255)                           ttATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGCTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAAtt                                                                                                                                                                                                         <  1:643840/251‑1 (MQ=255)                                 ccTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTtgatg                                                                                                                                                                                                    <  1:430162/250‑1 (MQ=255)                                  cTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAAttt                                                                                                                                                                                                        >  1:118852/1‑245 (MQ=255)                                         cATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAAc                                                                                                                                                                                           <  1:20569/251‑1 (MQ=255)                                               tCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATc                                                                                                                                                                                      <  1:821828/250‑1 (MQ=255)                                                      gCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATcaaccaac                                                                                                                                                                               <  1:886392/250‑1 (MQ=255)                                                           aaTCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTaa                                                                                                                                                                         >  1:3187/1‑251 (MQ=255)                                                            aTCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGat                                                                                                                                                                                                                                               >  1:793328/1‑180 (MQ=255)                                                            aTCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAAt                                                                                                                                                                        >  1:357541/1‑251 (MQ=255)                                                                   aCATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTa                                                                                                                                                                 >  1:753789/1‑251 (MQ=255)                                                                   aCATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTa                                                                                                                                                                 >  1:854142/1‑251 (MQ=255)                                                                            ggATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAAt                                                                                                                                                        >  1:621551/1‑251 (MQ=255)                                                                             gATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAAt                                                                                                                                                        >  1:32200/1‑250 (MQ=255)                                                                                tAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGt                                                                                                                                                    <  1:192667/251‑1 (MQ=255)                                                                                 aaaGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGtt                                                                                                                                                   >  1:672315/1‑251 (MQ=255)                                                                                  aagaagCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTtc                                                                                                                                                  <  1:736252/251‑1 (MQ=255)                                                                                        cTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGaa                                                                                                                                            >  1:794870/1‑251 (MQ=255)                                                                                            cGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACaa                                                                                                                                                            >  1:372227/1‑231 (MQ=255)                                                                                                gATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATc                                                                                                                                     >  1:798474/1‑250 (MQ=255)                                                                                                  tGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCaa                                                                                                                                  >  1:74691/1‑251 (MQ=255)                                                                                                       gTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGtt                                                                                                                                                   <  1:157834/229‑1 (MQ=255)                                                                                                          gtgtACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTg                                                                                                                          >  1:29023/1‑251 (MQ=255)                                                                                                            gtACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGaa                                                                                                                        >  1:246154/1‑251 (MQ=255)                                                                                                                   aGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCg                                                                                                                 >  1:589515/1‑251 (MQ=255)                                                                                                                      tCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATg                                                                                                                                                                       <  1:766208/194‑1 (MQ=255)                                                                                                                              cTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAAtt                                                                                                      <  1:749632/251‑1 (MQ=255)                                                                                                                                    ggCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCtt                                                                                                >  1:57994/1‑251 (MQ=255)                                                                                                                                                  catTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATcc                                                                                                                                    <  1:172833/201‑1 (MQ=255)                                                                                                                                                   tttttAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGt                                                                                 <  1:479204/250‑1 (MQ=255)                                                                                                                                                   atTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGt                                                                                 <  1:333635/251‑1 (MQ=255)                                                                                                                                                   atTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGt                                                                                 >  1:404520/1‑251 (MQ=255)                                                                                                                                                    ttttAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTa                                                                                >  1:518135/1‑251 (MQ=255)                                                                                                                                                       tAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTa                                                                             <  1:832697/251‑1 (MQ=255)                                                                                                                                                           cggcgACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACg                                                                           <  1:188287/249‑1 (MQ=255)                                                                                                                                                           cggcgACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACg                                                                           <  1:436095/249‑1 (MQ=255)                                                                                                                                                           cggcgACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGt                                                                          <  1:324435/250‑1 (MQ=255)                                                                                                                                                             gcgACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACaaaaa                                                                                                                                                         >  1:284847/1‑169 (MQ=255)                                                                                                                                                             gcgACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGt                                                                          >  1:766702/1‑248 (MQ=255)                                                                                                                                                                      cTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATTTGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTcac                                                               >  1:807065/1‑250 (MQ=255)                                                                                                                                                                            gTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTa                                                        >  1:888642/1‑251 (MQ=255)                                                                                                                                                                                 gAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCg                                                    <  1:518341/250‑1 (MQ=255)                                                                                                                                                                                    gCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTcac                                                               >  1:847702/1‑236 (MQ=255)                                                                                                                                                                                     ccATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAg                                               >  1:505453/1‑251 (MQ=255)                                                                                                                                                                                                    cgcAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGt                                 >  1:502678/1‑250 (MQ=255)                                                                                                                                                                                                    cgcAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGt                                 <  1:854167/250‑1 (MQ=255)                                                                                                                                                                                                        aGGCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAAt                                                                                                                                                        >  1:98937/1‑127 (MQ=255)                                                                                                                                                                                                          gCGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGAt                          >  1:328539/1‑251 (MQ=255)                                                                                                                                                                                                           cGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGAt                          <  1:312399/250‑1 (MQ=255)                                                                                                                                                                                                           cGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGAt                          <  1:792949/250‑1 (MQ=255)                                                                                                                                                                                                           cGTGATTTCCGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGAt                          <  1:226019/250‑1 (MQ=255)                                                                                                                                                                                                                    cGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGAt                          >  1:293932/1‑241 (MQ=255)                                                                                                                                                                                                                    cGTCACGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGATGCATTAAAAg                <  1:68027/251‑1 (MQ=255)                                                                                                                                                                                                                         cGAACAACGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGATGCATTAAAAgccgc            >  1:608743/1‑250 (MQ=255)                                                                                                                                                                                                                           aacaacGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGTGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGt                                                                          >  1:132844/1‑186 (MQ=255)                                                                                                                                                                                                                                cGTGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGATGCATTAAAAGCCGCCGgcttg     >  1:313383/1‑250 (MQ=255)                                                                                                                                                                                                                                  tGGCAGCCGCCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGATGCATTAAAAGCCGCCGgcttgctt  <  1:575793/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   ATGTACCGGGACGCACCGGCAGTGACTTATTGCCTGAAACCATCGGTCGTTTGGCAAAAATCAGCAACATTGTGGGGATTAAAGAAGCTACCGGCGATGTCAGTCGTGTACAAAAGATCAAACAACTTGCCGGCGAAGATTTCATCATTTTAAGCGGCGACGATGCTACCGGTTTGGAAGCCATGAAACTGGGCGCGCAAGGCGTGATTTCCGTCACGAACAACGTGGCAGCCACCGATATGGCAAAAATGTGCCATCTCGCCCGCGCGGGTAAATTTGATGAGGCAGAACAAATCAACCAACGCTTAATGCCGCTACACAAAAATCTGTTCGTCGAATCAAATCCAATTCCGGTGAAATGGGCGTGTTACAAATTAGGCTTGATTCAGGAGCCGGTATTACGTCTGCCGCTCACCACATTAAGCGAACAGGCACAGCCGAAAGTCTTAGATGCATTAAAAGCCGCCGGCTTGCTT  >  1/1612932‑1613407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑