Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,600,387	A→C	intergenic (‑159/‑44)	unknown ← / → unknown	UPF0381 protein YfcZ/Long‑chain fatty acid transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,600,387	0	A	C	100.0%	149.8 / NA	52	intergenic (‑159/‑44)	unknown/unknown	UPF0381 protein YfcZ/Long‑chain fatty acid transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/26);  total (26/26)

CAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATAAGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATGAGC  >  1/1600159‑1600616                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      cAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAAcc                                                                                                                                                                                                                 <  1:499769/251‑1 (MQ=255)           aCCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATaa                                                                                                                                                                                                       <  1:416094/251‑1 (MQ=255)              cACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAg                                                                                                                                                                                                    >  1:526507/1‑251 (MQ=255)                                  cTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAAcc                                                                                                                                                                                                                 <  1:28104/218‑1 (MQ=255)                                  cTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACaaaaa                                                                                                                                                                                           >  1:784968/1‑240 (MQ=255)                                                                 gCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTTCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACg                                                                                                                                                     >  1:491888/1‑247 (MQ=255)                                                                    cATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGttat                                                                                                                                              >  1:655400/1‑251 (MQ=255)                                                                      taataTCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGttatta                                                                                                                                            >  1:800749/1‑251 (MQ=255)                                                                      taataTCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGttatta                                                                                                                                            >  1:872074/1‑251 (MQ=255)                                                                                      aaaGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTg                                                                                                                            <  1:713012/251‑1 (MQ=255)                                                                                                      cAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAAc                                                                                                                                          >  1:239594/1‑221 (MQ=255)                                                                                                           aTTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATcc                                                                                                                                                        >  1:608157/1‑202 (MQ=255)                                                                                                           aTTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAAt                                                                                                       >  1:548521/1‑251 (MQ=255)                                                                                                                     tattCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCaa                                                                                                                                                                                                          <  1:28375/142‑1 (MQ=255)                                                                                                                      attCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGttt                                                                                                                                                                                                                                   <  1:647399/116‑1 (MQ=255)                                                                                                                      attCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTc                                                                                                                        <  1:10758/223‑1 (MQ=255)                                                                                                                      attCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAAtt                                                                                            >  1:598828/1‑251 (MQ=255)                                                                                                                           aaTTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCAc                                                                                       <  1:375174/251‑1 (MQ=255)                                                                                                                            aTTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCAc                                                                                       >  1:238971/1‑250 (MQ=255)                                                                                                                                                   cTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACaaaaa                                                                                                                                                                                           <  1:702825/127‑1 (MQ=255)                                                                                                                                                          ttGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGaa                                                        >  1:881169/1‑251 (MQ=255)                                                                                                                                                              ccACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAgccg                                                    >  1:126076/1‑251 (MQ=255)                                                                                                                                                                   ggTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACa                                                                                                                                                                                      <  1:98838/116‑1 (MQ=255)                                                                                                                                                                   ggTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAAc                                                                                                                                          >  1:596044/1‑160 (MQ=255)                                                                                                                                                                   ggTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGCGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCAt                                                >  1:437475/1‑250 (MQ=255)                                                                                                                                                                   ggTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGc                                                                                                               >  1:352441/1‑187 (MQ=255)                                                                                                                                                                   ggTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAAtt                                                                                                      <  1:25007/196‑1 (MQ=255)                                                                                                                                                                   ggTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCAt                                                >  1:632881/1‑250 (MQ=255)                                                                                                                                                                     tCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAAc                                                                                                                                          <  1:117997/158‑1 (MQ=255)                                                                                                                                                                             cTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAAcc                                                                                                                                                                                                                 >  1:557394/1‑79 (MQ=255)                                                                                                                                                                             cTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAAc                                                                                                                                          >  1:531533/1‑150 (MQ=255)                                                                                                                                                                                   aaaaTATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGctgctg                               <  1:880566/251‑1 (MQ=255)                                                                                                                                                                                      atatGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATcc                                                                                                                                                        <  1:853500/127‑1 (MQ=255)                                                                                                                                                                                      atatGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAAc                                                                                                                                          >  1:245232/1‑141 (MQ=255)                                                                                                                                                                                          gAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCg                         >  1:136037/1‑250 (MQ=255)                                                                                                                                                                                           aaaaGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGcc                       <  1:7858/251‑1 (MQ=255)                                                                                                                                                                                                    ttGCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGc                                             <  1:103654/220‑1 (MQ=255)                                                                                                                                                                                                      gCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAAc                                                                                                                                          <  1:146470/125‑1 (MQ=255)                                                                                                                                                                                                      gCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTgg                            <  1:625181/235‑1 (MQ=255)                                                                                                                                                                                                      gCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCgg             >  1:653592/1‑250 (MQ=255)                                                                                                                                                                                                      gCTCCACATTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCgg             >  1:453630/1‑250 (MQ=255)                                                                                                                                                                                                           acaTTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGt                                                                          <  1:812406/184‑1 (MQ=255)                                                                                                                                                                                                             aTTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATg     <  1:333163/251‑1 (MQ=255)                                                                                                                                                                                                             aTTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATg     <  1:598387/251‑1 (MQ=255)                                                                                                                                                                                                             aTTCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATg     <  1:526301/251‑1 (MQ=255)                                                                                                                                                                                                              ttCCCCTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATGa    >  1:501424/1‑251 (MQ=255)                                                                                                                                                                                                                   cTGTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATGAGc  >  1:258149/1‑248 (MQ=255)                                                                                                                                                                                                                     gTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAAcc                                                                                                                                                                                                                 >  1:788354/1‑39 (MQ=255)                                                                                                                                                                                                                     gTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTc                                                                                                                                                                      <  1:824489/82‑1 (MQ=255)                                                                                                                                                                                                                     gTTTTTTAGAATGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAAtt                                                                                                      <  1:345860/146‑1 (MQ=255)                                                                                                                                                                                                                               aTGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAAtgc                                    <  1:269476/202‑1 (MQ=255)                                                                                                                                                                                                                               aTGATACGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAAt      <  1:638209/232‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      CAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACACTACGCAAAATATGAAAAGAGGATTGCTCCACATTCCCCTGTTTTTTAGAATGATAAGTTTTCCTAAGAAACGATAACCAATTCAATAAAAGGTACAAAAATGACAACCCGCAAATTTACTCAAACCCTTCTATCCACGCTGTTATTAACTGCTGCCGGAGGTGCGTCCGCCGCGGCATTCCAATTAGCAGAAATTTCCACCTCCGGTTTAGGTCGTGCCTATGCCGGTGAAGCCGCCATCGCAGACAATGCTGCTGTGGTCGCCACCAACCCGGCATTAATGAGC  >  1/1600159‑1600616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑