Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,597,262	G→A	V62I (GTT→ATT)	unknown →	Putative secretion ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,597,262	0	G	A	100.0%	61.7 / NA	23	V62I (GTT→ATT)	unknown	Putative secretion ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

ATTTTCAATAGACTGCAAGTTTCTTTTTCCACCTATTAAAGTTACAAAATTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAGTTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGCTATTAAAGCGCCATCAGGCTTTGGAAAAACAACATTACTTAAGT  >  1/1597024‑1597502                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 attTTCAATAGACTGCAAGTTTCTTTTTCCACCTATTAAAGTTACAAAATTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGct                                                                                                                                                                                                                                          <  1:395891/247‑1 (MQ=255)                        tttttCCACCTATTAAAGTTACAAAATTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGAtata                                                                                                                                                                                                               <  1:634136/251‑1 (MQ=255)                                 cTATTAAAGTTACAAAATTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATag                                                                                                                                                                                                                                  >  1:854740/1‑223 (MQ=255)                                 cTATTAAAGTTACAAAATTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATag                                                                                                                                                                                                                                  >  1:472630/1‑223 (MQ=255)                                               aaaTTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAgaga                                                                                                                                                                                                                               <  1:390357/212‑1 (MQ=255)                                                                            tGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAAtt                                                                                                                                                                                                                        >  1:240045/1‑190 (MQ=255)                                                                                                      tACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAg                                                                                                                                 <  1:567924/251‑1 (MQ=255)                                                                                                                                                cAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGAt                                                                                                                                                                                       >  1:813922/1‑155 (MQ=255)                                                                                                                                                    gACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATaga                                                                                                                                                                                                                                 <  1:75549/109‑1 (MQ=255)                                                                                                                                                    gACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAAt                                                                                                                                                                                                      >  1:845982/1‑136 (MQ=255)                                                                                                                                                            gtatgtatGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATAcc                                                                                                                                                             >  1:800370/1‑169 (MQ=255)                                                                                                                                                                                          gTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAg                                               <  1:802778/249‑1 (MQ=255)                                                                                                                                                                                          gTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGc                                              <  1:815645/250‑1 (MQ=255)                                                                                                                                                                                                           aTGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAgaga                                                                                                                                                                                                                               >  1:90767/1‑56 (MQ=255)                                                                                                                                                                                                            tGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATGCCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGCTATTAAAGCGCCATCAGGc                           <  1:262767/251‑1 (MQ=255)                                                                                                                                                                                                            tGACAAACCTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGCTATTAAAGCGCCATCAgg                            >  1:790441/1‑250 (MQ=255)                                                                                                                                                                                                                   ccTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGaa                                                                                                                                                                            <  1:395865/99‑1 (MQ=255)                                                                                                                                                                                                                   ccTAATTAATATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATc                                                                                                           >  1:332188/1‑164 (MQ=255)                                                                                                                                                                                                                       attaatATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGCTATTAAAGCGCCATCAGGCTTTGGAAAaac                <  1:883530/251‑1 (MQ=255)                                                                                                                                                                                                                        ttaatATTTTCTTCAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATaa                                                                                                             <  1:409839/157‑1 (MQ=255)                                                                                                                                                                                                                                     cAATATAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGCTATTAAAGCGCCATCAGGCTTTGGAAAAACAACATTACTTAAGt  <  1:530561/251‑1 (MQ=255)                                                                                                                                                                                                                                       atatAAGAATTTTAGCTCTGTATAGCGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGAt                                                                                                                                                                                       >  1:610916/1‑68 (MQ=255)                                                                                                                                                                                                                                       atatAAGAATTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATtc                                                                                                                     <  1:95986/134‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 ATTTTCAATAGACTGCAAGTTTCTTTTTCCACCTATTAAAGTTACAAAATTAAACATGCTATTTTCTGGAATTCATGCCTCTATTGCGGCTTTAGAACAAATACTAATTTTATGGGTTGGTGAAACCCTAGTAATTGAAAATACAATGACATTGGGTATGTATGTTGCTTTTAATTCTTACCGAGGTTTATTTTCTTCACGTATGACAAACCTAATTAATATTTTCTTCAATATAAGAGTTTTAGCTCTGTATAGAGAACGAATTGCAGATATAGCACTAAATGAAGCAGAAGAAGATACTTATCAGAATTTGACGAATATACCTAAAAAAGTAGCAACACTCGAACTAAAGAATCTTACATTCTCTTATAATCAATTTGATAAACCGATAATAAAAGATATTAGTTTAATCATTCCTGCAGGTAAAAGTGTAGCTATTAAAGCGCCATCAGGCTTTGGAAAAACAACATTACTTAAGT  >  1/1597024‑1597502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑