Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,585,519	G→A	A80A (GCG→GCA)	unknown →	UPF0125 protein RatB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,585,519	0	G	A	100.0%	191.6 / NA	66	A80A (GCG→GCA)	unknown	UPF0125 protein RatB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/37);  total (29/37)

ATTCAAATTGCCTATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCGGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTG  >  1/1585298‑1585761                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   atTCAAATTGCCTATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACg                                                                                                                                                                                                                          >  1:53044/1‑248 (MQ=255)          gCCTATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACa                                                                                                                                                                                                              >  1:838172/1‑251 (MQ=255)           ccTATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACaa                                                                                                                                                                                                             <  1:460370/251‑1 (MQ=255)             tATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGc                                                                                                                                                                                                           <  1:35407/251‑1 (MQ=255)             tATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGc                                                                                                                                                                                                           <  1:594488/251‑1 (MQ=255)              aTGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCa                                                                                                                                                                                                          <  1:888123/251‑1 (MQ=255)                      ccTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGc                                                                                                                                                                                                           <  1:826217/242‑1 (MQ=255)                          atcatcATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAaata                                                                                                                                                                                               <  1:864096/250‑1 (MQ=255)                              tcatTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAAtaataa                                                                                                                                                                                           >  1:400964/1‑250 (MQ=255)                                atTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAacac                                                                                                                                                                                        <  1:529116/251‑1 (MQ=255)                                atTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAacac                                                                                                                                                                                        <  1:681524/251‑1 (MQ=255)                                      tGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTc                                                                                                                                                                                  <  1:411138/251‑1 (MQ=255)                                      tGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTc                                                                                                                                                                                  <  1:42784/251‑1 (MQ=255)                                      tGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTc                                                                                                                                                                                  <  1:680476/251‑1 (MQ=255)                                         aaTCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAac                                                                                                                                                                               <  1:386094/251‑1 (MQ=255)                                           tCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAacac                                                                                                                                                                             <  1:330387/251‑1 (MQ=255)                                           tCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAacac                                                                                                                                                                             <  1:875366/251‑1 (MQ=255)                                             gTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACa                                                                                                                                                                                                              >  1:497112/1‑216 (MQ=255)                                             gTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAacac                                                                                                                                                                             >  1:300647/1‑249 (MQ=255)                                             gTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACAt                                                                                                                                                                           >  1:125179/1‑251 (MQ=255)                                             gTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACAt                                                                                                                                                                           >  1:739649/1‑251 (MQ=255)                                                  gtgtAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAg                                                                                                                                                                      >  1:313597/1‑251 (MQ=255)                                                  gtgtAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAg                                                                                                                                                                      >  1:687237/1‑251 (MQ=255)                                                       ggTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATg                                                                                                                                                                 >  1:843351/1‑251 (MQ=255)                                                           aaGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGtata                                                                                                                                                             <  1:856708/251‑1 (MQ=255)                                                              gCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGCAATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAacac                                                                                                                                                                                        >  1:256505/1‑222 (MQ=255)                                                              gCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGa                                                                                                                                                          >  1:796250/1‑251 (MQ=255)                                                                 cTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTc                                                                                                                                                                                  <  1:412389/224‑1 (MQ=255)                                                                 cTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATg                                                                                                                                                                 >  1:360891/1‑241 (MQ=255)                                                                 cTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACa                                                                                                                                                       <  1:868731/251‑1 (MQ=255)                                                                   aTGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAgg                                                                                                                                                                     >  1:335151/1‑235 (MQ=255)                                                                     ggTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAGCGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAgg                                                                                                                                                                     <  1:186250/233‑1 (MQ=255)                                                                      gTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAAtt                                                                                                                                                   <  1:179901/250‑1 (MQ=255)                                                                                  aTTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCaa                                                                                                                                      >  1:183559/1‑251 (MQ=255)                                                                                     ttACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAat                                                                                                                                   <  1:571521/251‑1 (MQ=255)                                                                                     ttACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAAGTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAat                                                                                                                                   <  1:444851/251‑1 (MQ=255)                                                                                        cAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGATCAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATAtt                                                                                                                                >  1:354280/1‑251 (MQ=255)                                                                                               gCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAgg                                                                                                                                                                     <  1:835340/207‑1 (MQ=255)                                                                                                    ttACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAgg                                                                                                                                                                     >  1:27653/1‑202 (MQ=255)                                                                                                       caacaaTTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCa                                                                                                                                                                                                          <  1:824535/162‑1 (MQ=255)                                                                                                          caaTTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAGAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTa                                                                                                              >  1:550351/1‑251 (MQ=255)                                                                                                            aTTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAacac                                                                                                                                                                                        >  1:486880/1‑175 (MQ=255)                                                                                                               tACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAgg                                                                                                                                                                     >  1:7783/1‑191 (MQ=255)                                                                                                                 ccGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATg                                                                                                                                                                 <  1:447777/193‑1 (MQ=255)                                                                                                                         gATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAg                                                                                               >  1:604091/1‑251 (MQ=255)                                                                                                                                     aataaaatCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAat                                                                                                                                   <  1:397019/203‑1 (MQ=255)                                                                                                                                           atCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACCCTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAAtt                                                                             <  1:669555/251‑1 (MQ=255)                                                                                                                                                    tttCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTc                                                                                                                                                                                  >  1:613104/1‑142 (MQ=255)                                                                                                                                                          ccGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTAcc                                                               <  1:871603/251‑1 (MQ=255)                                                                                                                                                                   gAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTAt                                                      >  1:272992/1‑251 (MQ=255)                                                                                                                                                                      aTTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCgg                                                   <  1:551509/251‑1 (MQ=255)                                                                                                                                                                        tAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAacac                                                                                                                                                                             <  1:423672/127‑1 (MQ=255)                                                                                                                                                                                  gCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAAtaataa                                                                                                                                                                                           >  1:541351/1‑103 (MQ=255)                                                                                                                                                                                  gCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTc                                                                                                                                                                                  <  1:802965/112‑1 (MQ=255)                                                                                                                                                                                  gCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACTTTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAAc                                        >  1:288748/1‑250 (MQ=255)                                                                                                                                                                                  gCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTatat                                                                                                                                                            >  1:751990/1‑134 (MQ=255)                                                                                                                                                                                   cTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAAcc                                       <  1:782606/250‑1 (MQ=255)                                                                                                                                                                                   cTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAAc                                        <  1:519257/249‑1 (MQ=255)                                                                                                                                                                                   cTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAAc                                        <  1:893181/249‑1 (MQ=255)                                                                                                                                                                                                                       cTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAgg                                                                                                                                                                     >  1:318746/1‑88 (MQ=255)                                                                                                                                                                                                                       cTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGc                                                                                                 <  1:95049/156‑1 (MQ=255)                                                                                                                                                                                                                       cTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTg  >  1:829328/1‑251 (MQ=255)                                                                                                                                                                                                                       cTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTg  <  1:678246/251‑1 (MQ=255)                                                                                                                                                                                                                       cTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTg  <  1:783314/251‑1 (MQ=255)                                                                                                                                                                                                                       cTGTTGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTg  >  1:727441/1‑251 (MQ=255)                                                                                                                                                                                                                           tGGCAGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAATACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGcc                 <  1:442269/232‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   ATTCAAATTGCCTATGCGTTTCCTGATCATCATTATCTGAAATCGTTTAGTGTAGGTGAAGGCACTATGGTGCAAACCGCCATTTTACAGTCCGGCATTTTACAACAATTTACCGACATTGATTTACGCGAAAATAAAATCGGC‑ATTTTCAGCCGTCCCGTGAAATTAACGGATCAGCTGAAAGACGGCGATCGCATTGAAATTTATCGCCCGCTGTTGGCGGATCCGAAAGAAATTCGACGCAAACGTGCGGAAGAACAAGCAAAGAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTG  >  1/1585298‑1585761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑