Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,584,971	T→G	L38R (CTC→CGC)	unknown →	Ribosome association toxin RatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,584,971	0	T	G	100.0%	206.6 / NA	73	L38R (CTC→CGC)	unknown	Ribosome association toxin RatA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (45/28);  total (45/28)

CTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCTCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAATTTTCAAATCCACCGGTTGGATTTGCCTT  >  1/1584726‑1585211                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTGTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCGCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGt                                                                                                                                                                                                                                              <  1:653892/251‑1 (MQ=255)                                       aTCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGaa                                                                                                                                                                                                       >  1:751388/1‑251 (MQ=255)                                        tCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGaaa                                                                                                                                                                                                      >  1:607095/1‑251 (MQ=255)                                        tCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTGTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTtaaata                                                                                                                                                                                                                                        <  1:111612/218‑1 (MQ=255)                                             aaCCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAg                                                                                                                                                                                                  >  1:139168/1‑250 (MQ=255)                                                             ttttAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑g                                                                                                                                                                                                                                   <  1:704130/201‑1 (MQ=255)                                                                    ctcctcATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGtt                                                                                                                                                                          >  1:638502/1‑251 (MQ=255)                                                                      cctcATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTa                                                                                                                                                                        >  1:167618/1‑251 (MQ=255)                                                                                   cAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:34026/251‑1 (MQ=255)                                                                                   cAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:601316/251‑1 (MQ=255)                                                                                         ttCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATgaa                                                                                                                                                     >  1:747352/1‑251 (MQ=255)                                                                                                ataataTACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTaccacc                                                                                                                                                                   <  1:594143/230‑1 (MQ=255)                                                                                                ataataTACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:364323/238‑1 (MQ=255)                                                                                                ataataTACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAaat                                                                                                                                              >  1:464823/1‑251 (MQ=255)                                                                                                ataataTACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTACATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAaat                                                                                                                                              >  1:505792/1‑251 (MQ=255)                                                                                                 taataTACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAaat                                                                                                                                              >  1:7881/1‑250 (MQ=255)                                                                                                             tttttCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGaa                                                                                                                                 <  1:354162/251‑1 (MQ=255)                                                                                                                    tCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAAtt                                                                                                                          >  1:677627/1‑251 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTaccacc                                                                                                                                                                   >  1:618742/1‑204 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTacca                                                                                                                                                                     >  1:601831/1‑202 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           >  1:375242/1‑212 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACaa                                                                                                                                                         <  1:713225/214‑1 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAg                                                                                                                                   <  1:166634/236‑1 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGa                                                                                                                    >  1:159286/1‑251 (MQ=255)                                                                                                                          aCCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGa                                                                                                                    >  1:653691/1‑251 (MQ=255)                                                                                                                               aaTCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGt                                                                                                                >  1:530961/1‑250 (MQ=255)                                                                                                                                  cTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:96899/204‑1 (MQ=255)                                                                                                                                  cTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATgaaaga                                                                                                                                                  >  1:637887/1‑213 (MQ=255)                                                                                                                                  cTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGt                                                                                                            >  1:392185/1‑251 (MQ=255)                                                                                                                                  cTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGt                                                                                                            >  1:647979/1‑251 (MQ=255)                                                                                                                                    gTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:785508/202‑1 (MQ=255)                                                                                                                                    gTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATgaaagaa                                                                                                                                                 >  1:201621/1‑212 (MQ=255)                                                                                                                                          cTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAAtt                                                                                                     <  1:28847/250‑1 (MQ=255)                                                                                                                                            aTTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAAttt                                                                                                    >  1:360301/1‑249 (MQ=255)                                                                                                                                             ttGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTaccacc                                                                                                                                                                   <  1:354912/185‑1 (MQ=255)                                                                                                                                             ttGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACaa                                                                                                                                                         <  1:60964/195‑1 (MQ=255)                                                                                                                                             ttGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGGTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAAttt                                                                                                    >  1:684361/1‑248 (MQ=255)                                                                                                                                              tGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTg                                                                                                 >  1:819854/1‑250 (MQ=255)                                                                                                                                                       atcGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGaaa                                                                                       >  1:494732/1‑251 (MQ=255)                                                                                                                                                                cGTTGCATACAACGCTCAACAAATGTATCTGCTTTTAAATGATTATGAGCGTTATCCCGATTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:597741/174‑1 (MQ=255)                                                                                                                                                                cGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTac                                                                                                                                                                       <  1:613374/162‑1 (MQ=255)                                                                                                                                                                cGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           >  1:836273/1‑174 (MQ=255)                                                                                                                                                                cGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCg                                                                              >  1:241919/1‑251 (MQ=255)                                                                                                                                                                 gTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAAc                                                                                                                                                                                                                    <  1:449797/116‑1 (MQ=255)                                                                                                                                                                 gTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTATGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAAt                                                                                                                           >  1:494939/1‑205 (MQ=255)                                                                                                                                                                   tGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCgg                                                                                                                                                                                                         >  1:373383/1‑125 (MQ=255)                                                                                                                                                                    gCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAAt                                                                                                      <  1:403336/223‑1 (MQ=255)                                                                                                                                                                    gCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATc                                                                           <  1:80417/250‑1 (MQ=255)                                                                                                                                                                                caacaaATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAg                                                                                     >  1:526570/1‑228 (MQ=255)                                                                                                                                                                                  acaaATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           <  1:861764/156‑1 (MQ=255)                                                                                                                                                                                   caaATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAAt                                                                                                                           <  1:140869/187‑1 (MQ=255)                                                                                                                                                                                       tGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAAtt                                                                                                     >  1:148443/1‑205 (MQ=255)                                                                                                                                                                                        gTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGa                                                      >  1:241025/1‑251 (MQ=255)                                                                                                                                                                                            tataaaagaaaggATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAAGGTG‑GGTGCAATTAACCGGGGAAGCCGAAATCAGGAAGGCGGGGATTCGGAAAAAATTTAACACCCGTAATAAAATGAAAGAAAATCAATCAATTAGAATGCAGTTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCg                                                  <  1:133866/239‑1 (MQ=255)                                                                                                                                                                                              gCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGa                                                 >  1:373501/1‑250 (MQ=255)                                                                                                                                                                                                ttttAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACtt                                              >  1:602690/1‑251 (MQ=255)                                                                                                                                                                                                 tttAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACttt                                             >  1:343270/1‑251 (MQ=255)                                                                                                                                                                                                    aaaTGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATAc                                                                                                                                                           >  1:834923/1‑138 (MQ=255)                                                                                                                                                                                                     aaTGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACtttattt                                         <  1:370831/251‑1 (MQ=255)                                                                                                                                                                                                        gATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATgaaag                                                                                                                                                   >  1:86811/1‑142 (MQ=255)                                                                                                                                                                                                                gCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAAt                              >  1:206195/1‑251 (MQ=255)                                                                                                                                                                                                                     aTCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACaa                                                                                                                                                         >  1:638190/1‑123 (MQ=255)                                                                                                                                                                                                                     aTCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGt                                                                                    >  1:45737/1‑192 (MQ=255)                                                                                                                                                                                                                      tCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGc                                                                                                                              >  1:15518/1‑149 (MQ=255)                                                                                                                                                                                                                       cccGAGTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCa                                     >  1:594740/1‑237 (MQ=255)                                                                                                                                                                                                                            gTTTTTGCCAGGCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGc                                      <  1:292477/231‑1 (MQ=255)                                                                                                                                                                                                                                       gCTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAATTTTCAAATCCACCGGTTGGa          <  1:759830/248‑1 (MQ=255)                                                                                                                                                                                                                                        cTGTGTCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACtttat                                           >  1:151824/1‑214 (MQ=255)                                                                                                                                                                                                                                          gtgtCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAg                                       >  1:35827/1‑216 (MQ=255)                                                                                                                                                                                                                                          gtgtCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAATTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAATTTTCAAATCCACCGGTTGGATTTGc     >  1:455596/1‑250 (MQ=255)                                                                                                                                                                                                                                           tgtCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAATTTTCAAATCCACCGGTTGGATTTGcc    <  1:464963/250‑1 (MQ=255)                                                                                                                                                                                                                                            gtCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAaatc                                                                                                                                             <  1:323706/112‑1 (MQ=255)                                                                                                                                                                                                                                            gtCGGCAACCGCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAATTTTCAAATCCACCGGTTGGATTTGCCtt  >  1:479266/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAACTTTTTCATCCACAACCTTAATCTGTATGGCTATTGTTAATCAATCGACGCTCGTTGCATACAACGCGCAACAAATGTATCGGCTTTTAAATGATTATGAGCGTTATCCCGAGTTTTTGCCAGGCTGTGTCGGCAACCTCACGTTAAATAAAA‑GTGCGGTGCAATTAACCGGTGAATCGGAAATCAGCAAGGCGGGCATTCGACAAAAGTTTACCACCTGTAATACAATGAAAGAAAATCAATCAATTAGAATGCAATTCGTTGACGGTCCGTTTAAATTTCTGCAAGGTGAAAAGTTTACCGATCCGGATAAAAAAAGCTGTCAGACTCGACTTTATTTAGCATCTGAATTTTCAAATCCACCGGTTGGATTTGCCTT  >  1/1584726‑1585211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑