Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 217,314 A→C intergenic (+58/‑96) unknown → / → unknown 2‑dehydrotetronate isomerase (EC 5.3.1.35)/Lysophospholipase (EC 3.1.1.5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1217,3140AC100.0% 106.8 / NA 39intergenic (+58/‑96)unknown/unknown2‑dehydrotetronate isomerase (EC 5.3.1.35)/Lysophospholipase (EC 3.1.1.5)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/21);  total (18/21)

CGCATGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCACTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCATCCCGGTCTCCATAATTA  >  1/217081‑217393
                                                                                                                                                                                                                                         |                                                                               
cgcATGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAGTACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGc                                                                <  1:354207/251‑1 (MQ=255)
    tGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAg                                                            >  1:164299/1‑251 (MQ=255)
     gTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATcc                                                                     <  1:573903/241‑1 (MQ=255)
               cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:827849/251‑1 (MQ=255)
               cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:499309/251‑1 (MQ=255)
               cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:331235/251‑1 (MQ=255)
               cTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTAt                                                 <  1:360720/251‑1 (MQ=255)
                   cGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCt                                             <  1:726901/251‑1 (MQ=255)
                             cGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   <  1:252091/251‑1 (MQ=255)
                             cGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   <  1:43794/251‑1 (MQ=255)
                              gTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      <  1:632890/215‑1 (MQ=255)
                              gTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAgg                                                           >  1:781365/1‑226 (MQ=255)
                              gTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATa                                    >  1:569986/1‑249 (MQ=255)
                                  tGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTAtt                              <  1:86089/251‑1 (MQ=255)
                                    aaCCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      <  1:807898/209‑1 (MQ=255)
                                    aaCCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAg                                                         <  1:325952/222‑1 (MQ=255)
                                    aaCCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTg                                                    >  1:5689/1‑227 (MQ=255)
                                                            aTGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      <  1:384494/185‑1 (MQ=255)
                                                            aTGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             <  1:125963/226‑1 (MQ=255)
                                                               aaTACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacgagcgttgcagcac  <  1:450944/250‑18 (MQ=255)
                                                                              aaTTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             >  1:130264/1‑208 (MQ=255)
                                                                                                        ttCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGc                                                                <  1:133245/147‑1 (MQ=255)
                                                                                                        ttCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACtt                                 >  1:401412/1‑178 (MQ=255)
                                                                                                                      gTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacg               >  1:849206/1‑178 (MQ=255)
                                                                                                                             ccACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacgagcgttgcagcac  >  1:719537/1‑171 (MQ=255)
                                                                                                                                                   gTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGc                                                                <  1:303084/104‑1 (MQ=255)
                                                                                                                                                       aGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCAtaacg               <  1:404948/149‑5 (MQ=255)
                                                                                                                                                            ggttTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTtat                                                                         >  1:464338/1‑86 (MQ=255)
                                                                                                                                                            ggttTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTc                                              >  1:375841/1‑113 (MQ=255)
                                                                                                                                                                 gAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATc                                                                      >  1:653142/1‑84 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAgg                                                           <  1:271094/90‑1 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTa                                                  >  1:533406/1‑99 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATg                                         >  1:226797/1‑108 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGaaa                                      >  1:789542/1‑111 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   >  1:827047/1‑114 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATAc                                   <  1:597136/114‑1 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             >  1:190763/1‑120 (MQ=255)
                                                                                                                                                                      gTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTc                             >  1:118930/1‑120 (MQ=255)
                                                                                                                                                                               aGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCCCTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAAt                         >  1:401954/1‑115 (MQ=255)
                                                                                                                                                                                                                                         |                                                                               
CGCATGTACAAATTGCTTCCGTACCGGATCGTCATGAACCGGATGAAGGCGAAATCAATTATGAATACCTCTTTAATAAATTAGATGAGATTGGTTACCAAGGTTTCGTAGGCTGTGAGTACAAACCACGGGGTGAAACCTCAAGCGGTCTAGGTTGGTTTGAACAGTATAAATAAGTGATGAAGTGCGGTTGTTTTTTTGAGAGTTTTTAAAACGTCATGAAAATTGACCGCACTTTTTATATCCATAGCTAAGGAGTTTTGTATGTCTAATGAAATACTTATTCTAATTGGCATCCCGGTCTCCATAATTA  >  1/217081‑217393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑