Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,532,385	A→G	L46L (TTG→CTG)	unknown ←	Putative DNA‑binding protein in cluster with Type I restriction‑modification system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,532,385	0	A	G	100.0%	184.8 / NA	62	L46L (TTG→CTG)	unknown	Putative DNA‑binding protein in cluster with Type I restriction‑modification system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/38);  total (24/38)

TGTAAGCGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAAGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGAAAAGCAAAACACCGAAAAAGATGACCGCACTTAAATCGCTACGCAGATCACAAA  >  1/1532138‑1532630                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tGTAAGCGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCt                                                                                                                                                                                                                                                    <  1:452187/251‑1 (MQ=255) tGTAAGCGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCt                                                                                                                                                                                                                                                    <  1:685969/251‑1 (MQ=255)       cGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:299333/250‑1 (MQ=255)       cGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:143639/250‑1 (MQ=255)       cGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:770047/250‑1 (MQ=255)       cGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:368574/250‑1 (MQ=255)              cATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCtata                                                                                                                                                                                                                                       >  1:639790/1‑251 (MQ=255)               aTTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAAtatta                                                                                                                                                                                                                                             <  1:306527/244‑1 (MQ=255)               aTTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATAc                                                                                                                                                                                                                                      <  1:801456/251‑1 (MQ=255)                    aTGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:147725/237‑1 (MQ=255)                      gCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:836509/235‑1 (MQ=255)                      gCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               >  1:784504/1‑235 (MQ=255)                      gCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               >  1:246489/1‑235 (MQ=255)                      gCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATc                                                                                                                                                                                                                                           <  1:897888/239‑1 (MQ=255)                      gCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTa                                                                                                                                                                                                                                 >  1:137921/1‑249 (MQ=255)                          aaCCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAaat                                                                                                                                                                                                                           <  1:496399/251‑1 (MQ=255)                           aCCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTAc                                                                                                                                                                                                                                >  1:847411/1‑245 (MQ=255)                               tGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAAttt                                                                                                                                                                                                                      <  1:900118/251‑1 (MQ=255)                                gTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACt                                                                                                                                                                                                                                     >  1:636545/1‑235 (MQ=255)                                  aCCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAaat                                                                                                                                                                                                                           <  1:809115/243‑1 (MQ=255)                                                      ttGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGcc                                                                                                                                                                                               <  1:732363/251‑1 (MQ=255)                                                      ttGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGcc                                                                                                                                                                                               <  1:556095/251‑1 (MQ=255)                                                        gtgtAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCt                                                                                                                                                                                                                                                    >  1:802019/1‑196 (MQ=255)                                                        gtgtAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACt                                                                                                                                                                                                                                     <  1:174508/211‑1 (MQ=255)                                                                    gACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCtt                                                                                                                                                                                  >  1:293834/1‑250 (MQ=255)                                                                        gAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAGGGTGCAGGCTAATATTATCTATACTTGTACTAaat                                                                                                                                                                                                                           >  1:448125/1‑205 (MQ=255)                                                                        gAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               <  1:272499/185‑1 (MQ=255)                                                                           ttGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAg                                                                                                                                                                          <  1:232177/251‑1 (MQ=255)                                                                                       cTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               >  1:126122/1‑170 (MQ=255)                                                                                            aaCTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAAcc                                                                                                                                                         <  1:535784/251‑1 (MQ=255)                                                                                                          ccGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACt                                                                                                                                           <  1:619006/251‑1 (MQ=255)                                                                                                          ccGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAAAATATATTTTTAAGGTGCAGGTTAATATTATCTATACTTGTACTGACTAATTTCGCCATTTCAGGCTAGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACt                                                                                                                                           <  1:746571/251‑1 (MQ=255)                                                                                                                          cATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               >  1:858697/1‑135 (MQ=255)                                                                                                                          cATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTAtt                                                                                                                           <  1:76035/251‑1 (MQ=255)                                                                                                                                  ttGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTAtt                                                                                                                   >  1:113598/1‑251 (MQ=255)                                                                                                                                       atatTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTg                                                                                                              >  1:253828/1‑251 (MQ=255)                                                                                                                                         atTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatatc                                                                                                                                                                                                                                              <  1:231828/121‑2 (MQ=255)                                                                                                                                         atTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGt                                                                                                                                                                   <  1:63876/196‑1 (MQ=255)                                                                                                                                                tGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATcc                                                                                                     <  1:376623/251‑1 (MQ=255)                                                                                                                                                   aCGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAatat                                                                                                                                                                                                                                               >  1:718617/1‑110 (MQ=255)                                                                                                                                                   aCGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGAc                                                                                                                                                                                                         <  1:338300/148‑1 (MQ=255)                                                                                                                                                    cGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTAt                                                                                                 >  1:476561/1‑251 (MQ=255)                                                                                                                                                          aCCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCt                                                                                                                                                                                                                                                    <  1:328141/98‑1 (MQ=255)                                                                                                                                                          aCCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACt                                                                                                                                           >  1:275118/1‑203 (MQ=255)                                                                                                                                                           ccTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCg                                                                                                                                                                                         <  1:390294/156‑1 (MQ=255)                                                                                                                                                              tACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAAcc                                                                                       <  1:883871/251‑1 (MQ=255)                                                                                                                                                                          cttGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGcc                                                                                                                                                                                               >  1:857807/1‑135 (MQ=255)                                                                                                                                                                            tGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATc                                                                         <  1:653975/251‑1 (MQ=255)                                                                                                                                                                                     aCCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAAt                                                                                                           <  1:83075/208‑1 (MQ=255)                                                                                                                                                                                             aTCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACt                                                                                                                                                                                                                                     <  1:504257/78‑1 (MQ=255)                                                                                                                                                                                             aTCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGa                                                        >  1:175483/1‑251 (MQ=255)                                                                                                                                                                                                      aGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAAc                                                                                        <  1:105655/210‑1 (MQ=255)                                                                                                                                                                                                                  atcaAGCTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGAAAAGCAAAACACCGAAAAAGa                                   <  1:534456/251‑1 (MQ=255)                                                                                                                                                                                                                        cTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAAtt                                                                                                                                 >  1:660720/1‑151 (MQ=255)                                                                                                                                                                                                                        cTCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCa                                                                        >  1:868492/1‑208 (MQ=255)                                                                                                                                                                                                                         tCATGCTCCAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAAttt                                                                                                                                <  1:288678/151‑1 (MQ=255)                                                                                                                                                                                                                                ccAAATATATATTTTTAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGAAAAGCAAAACACCGAAAAAGATGACCGCACTTaaa                     >  1:602588/1‑251 (MQ=255)                                                                                                                                                                                                                                           tttttAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGAt                                                               <  1:846539/198‑1 (MQ=255)                                                                                                                                                                                                                                           tttttAAGGTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAACCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGAAAAGCAAAACACCGAAAAAGATGACCGCACtt                        >  1:109056/1‑237 (MQ=255)                                                                                                                                                                                                                                                   gTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACt                                                                                                                                           >  1:816726/1‑114 (MQ=255)                                                                                                                                                                                                                                                   gTGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATg                                                                                                >  1:831669/1‑157 (MQ=255)                                                                                                                                                                                                                                                    tGCAGGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGAAAAGCAAAACACCGAAAAAGATGACCGCACTTAAATCGCTACGCAGATCACaaa  <  1:521197/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TGTAAGCGTCGATCATTTAATGCGTAACCCTGTACCAAATATTGCTTCAAGGTTTGTGTAGCCCATTGACGGAATTGCGTTGCCCGCTTAGAACTAATTCGATATCCGACGGAAATGATAGCATCGAGATTGTAATATTTAATTGTACGCTTAACCTTACGTTTACCTTCTTGTCGAACTACCGAGAAATCCTCGGTAGTTAAAATTTCATCAAGCTCATGCTCCAAATATATATTTTTAAGGTGCAAGCTAATATTATCTATACTTGTACTAAATAATTTCGCCATTTCAGACTGGCTAAGCCAAACCGTCTCCTTCTCAAAAGACACGGTTAACGAAACCTTCCCGTCCTGACTAGTGTAAATTTGTATTTGGTTATTCATTGAATTAATCCTTATGCCATAAACCGACTCAATATAATCAGCAATAGATTATCAGAAAAGCAAAACACCGAAAAAGATGACCGCACTTAAATCGCTACGCAGATCACAAA  >  1/1532138‑1532630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑