| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,522,037 | A→G | R309G (AGA→GGA) | unknown → | Autoinducer 2 (AI‑2) kinase LsrK (EC 2.7.1.‑) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,522,037 | 0 | A | G | 93.9% | 197.7 / ‑4.2 | 82 | R309G (AGA→GGA) | unknown | Autoinducer 2 (AI‑2) kinase LsrK (EC 2.7.1.‑) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (40/37); minor base C (5/0); total (45/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AAGTTGCACAACAAACCGGTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCAGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGACAAATGCAACAAAGCCGTATTATTC > 1/1521795‑1522276 | aaGTTGCACAACAACCCGGTTT‑AATACAGGGCACCCCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTGGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGCCCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAAcc < 1:467790/251‑1 (MQ=255) ttGCACAACAAACCGGTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑GAATGAATGTACGGATTAAACCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTTACCGGATTAACCATg > 1:637073/1‑251 (MQ=255) cacaACAAACCGGTTT‑AATACAGGGCACCCCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGGGACCGACCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATgcgc < 1:836652/251‑1 (MQ=255) caacaaACCGGTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGGGAATTTACCGCAGGCGGGGACCGACCCGG‑GAATTAATGTACGTATTAAACCGCACGTTATCCCGCCGTTAGGACAGGCGGGATCCATTAGCTTTTTCACCGGATTaaacctgcg > 1:222650/1‑241 (MQ=255) acaaCCCGGTTT‑AATACAGGGCACCCCGGTTGTGGTCGGCGGCGGGGGCGTGCAGTTGGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGGGGCAGTTATGGGCGGTACGTTCTGGCAACAAGGGGTGAATTTACCGCAGGCGGTGACCGACCCGA‑AAAGGAAGGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGt < 1:119786/251‑1 (MQ=255) aaaCCGGTTT‑AATACAGGGCACCCCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTc < 1:68829/251‑1 (MQ=255) ccGGTTTAAATAGAGGGCACACCGGTTGGGGACGGCGGCGGGGGCGTGCATTAAGGTT‑TATTTGTTTTAGGCGTCCCCGAGCCCGCAAAGGCGGGATTTGTCGGCGGTACGTTCTGAAAACAAGTGGAAAATTTACGGGGGGCGGTGACCGACCGGG‑AAATAAATGTACGTATTACCCCGCATGTTAGCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTc < 1:236802/249‑1 (MQ=255) gTTT‑AATACGGGGCACACGGGTTGTGGTCGGCGGGGGGGACGTGCAGTTAGGTTGTT‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGGGGTACGTTCTGGAAACAAGTGGTGAATTTACCGCAGGCGTTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAAcc < 1:341460/233‑1 (MQ=255) gTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTTTGGCAACAAGTGGTGAATTTACCGCAGGCGGGGACCGACCCGG‑AAAAGAATGTAAGTATTAACCCGCCCGTTATCCCCCCGTTAGTACAGGCGGGATCCATTAACCTTTTCACCCGGTTAAcc > 1:461177/1‑233 (MQ=255) gTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑GAATGAATGTACGTATTAACCCGCACGTTATTCCGCCGTTTGTACAGGCGGGATCCATTAGCTTTTTTACCGGATTAACCATGCGCTGGGTCCGTGAt > 1:233501/1‑251 (MQ=255) gTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCCACCCGG‑AAATGAATGTACGTATTAACCCCCACGTTATTCCGCCGTTAATACCGGCGGGATCCATTAACTTTTTTACCCGATTTACCATGCGCTGGTTTCGTGa > 1:520685/1‑250 (MQ=255) gTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGGGACCGACCCGG‑AAATGAATGTACGTATTAAACCCCACGGTATCCCCCCGGTAGTACAGGCGGGAACCATTAGCTTTTTCACCGGATTAACCATGCGCTGGGTCCGTGAt > 1:282141/1‑251 (MQ=255) aaTACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGCGGTGAATTTACCGCAGGGGGGGACCGACCCGG‑GAATGAAAGGACGGATTTACCCGGACGTTATTTCGCCGTTAGTACAGGCGGAATCCAATTGCTTTTTTACCGGGTTAACCCTCGGCTGGGTCCGTGGTGc > 1:144587/1‑249 (MQ=255) aaTACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGGTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCCACCCGG‑GAATGAATGGACGTATTAAACCCCACGTTTTCCCCCCCGTAGTACAGGCGGGATCCATTAGCTTTTTTACCGGATTAAACATGCGCTGGTTCCGGGATGCAt > 1:144574/1‑251 (MQ=255) aTACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGAAACAAGTGGTGAATTTCCCGCGGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTACCCCGCGCGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAAcc < 1:101774/228‑1 (MQ=255) gggCACCCCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGTTTGTA‑TTGGTTTAGGCGTCCCCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTCCCGCAGGCGGTGACCGCCCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGa < 1:890642/240‑1 (MQ=255) gggCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGGGACCCACCCGG‑GAAAGAATGTACGGATTAACCCGCACATTTTCCCGCCGTTAGTGCAGGCGGGATCCATTAGCTTTTTCACCGGGTTAACCATGCGCTGGGTCCCTGATGCCtttttcg > 1:447470/1‑248 (MQ=255) gggCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGGTCTGGCAACAAGGGGTGAATTTACCGCAGGCGGGGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTTACCGGATTAAcc > 1:600342/1‑223 (MQ=255) ggTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTTTTGCAACAAACGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGGTCGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGGATCCATTAACCTTTTCACCGGATTTACCATGCGCTGGTTCCGTGATGCATTTTTCGAAGAAGaaa > 1:616115/1‑251 (MQ=255) ggTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGGTCTGGCAACAAGGGGGGAAATTACCGCAGGCGGGGACCGAACCGG‑GAATGAATGGAAGGATTAAACCGCAAGTTATTCCGCCGTTAGTACAGGCGGGATTCATTAGCTTTTTCACCGGATTAACCATGCGCTGGGTTCGTGGTGGATTTTGCGAAGGAGAAAAgagaa > 1:519349/1‑250 (MQ=255) cAGTTAGGTTGTA‑TTGGTTTAGGCGTCCCCGGGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCTCCGGATTAACCATGCGCTGGTTc < 1:377442/201‑1 (MQ=255) cAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGGATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCCTTTTTCGAAGAAGAAAAAAGACTGGCGGGAAAAATgggga > 1:593765/1‑248 (MQ=255) cAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGGGACCGACCCCG‑AAATGAATGTAAGTATTAACCCGCACGGTATCCCGCCGTTAGTACAGGCGGGATCCATTAGCTTTTTTACCGGATTAACCATGCGCTGGTTTCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGGAAAAATggggaa > 1:278080/1‑248 (MQ=255) gTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTCCCGCAGGCGGTGCCCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCg < 1:540491/201‑1 (MQ=255) gtttGTA‑TTGTTTTAGGCGTCCCCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTCCCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACTTATTACCCCGCACTTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCa < 1:123697/182‑1 (MQ=255) gTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGCCCGACCCGG‑AAATGAATGTACGTATTACCCCGCACTTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGtt < 1:485939/193‑1 (MQ=255) gTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGGACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGGAACGATg > 1:712825/1‑249 (MQ=255) ttGTT‑TTGGTTTAGGCGTCCCCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACTTTCTGGCAACAAGTGGTGATTTTCCCGCAGGCGGTGACCGACCCGA‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCtt < 1:130202/251‑1 (MQ=255) gTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCCTTAGCTTTTTCACCGGATTAACCCTGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGGACCGATGCTTAt > 1:708538/1‑251 (MQ=255) gTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGGTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATgcgc > 1:806407/1‑185 (MQ=255) tatgggTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATg < 1:777379/247‑1 (MQ=255) tttGGTTTAGGCGTCCCCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGc < 1:760569/250‑1 (MQ=255) gttttAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAag < 1:443246/211‑1 (MQ=255) ggTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGTTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCCCACGTTATCCCCCCGTTAATACAGGCGGGATCCATTAGCTTTTTCACCCGGTTAACCATGCGCTGGTTCCCTGATTCCTTTTGCGAAGAAGAAAAGAGACTGGCGGGAAAAATggggtccgctgctttttcgtt > 1:853464/1‑233 (MQ=255) ggTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑GAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGGTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGGAAAACTGGGTACCGATGCTTATGCGt > 1:140912/1‑250 (MQ=255) ggTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGGATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAag > 1:165128/1‑213 (MQ=255) ggTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGGGACCCACCCGG‑GAATGAATGTACGGATTAACCCCCACGTTATCCCGCCGCTAGGACAGGCGGGATCCATTAGCTTTTTCACCGGATTAACCATGCGCCGGGTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGc > 1:425313/1‑221 (MQ=255) ggTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGCTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATTTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTATCCTTTTCACCCGATTAAACATTCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACCGGCGGAAAAACTGGGTACCCATGCTTATGCGtt > 1:14595/1‑251 (MQ=255) gTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGGTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAAATggggaacgaccgtctcttttaa > 1:226037/1‑232 (MQ=255) aGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑GAATGAATGTACGGATTAACCCGCACGTTATCCCGCCGGTAGTACAGGCGGGATCCATTAGCTTTTTTACCCGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGGAAAAATGGGTACCGATGCTTATTCGGTGCTgg > 1:756203/1‑251 (MQ=255) cACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTTGCAACAAGAGGTGAATTTACCGCAGGCGGGGACCGACCCCG‑GAATGAATGTACGGATTAACCCGCCCGTTATCCCCCCGTTAGTACAGGCGGGATCCATTAGCTTTTTCACCGGGTTAAACATGCGCTGGTTCCCTGATGCCTTTTTCGAAGAAGAAAAGAGACTGGCGGAAAAAATGGGGACCGATGCTTATGCGTTGCTGGAACaaa > 1:394764/1‑251 (MQ=255) cACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGGGAATTTACCGCAGGCGGTGACCGACCCGG‑GAATGAAAGTAAGTATTAAACCGGAAGGTATCCCGCCGGTAGTTTAGGCGGAAAACATTAGATTTTTTAACGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGTGAAGAATAAAAAAGACTGGCGGGAAAACTGGGGAACGATGTTTATGCggttgcggaaaaaa > 1:221363/1‑238 (MQ=255) gCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGAACGACCCGG‑AAATGAATGTACGTATTAACCCGCACGGTATTCCGCCGTTAGTACAGGGGGGATCCATTAGCCTTTTCACCGGATTAACCATGCGCTGGTTCCGGGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGGAAAACAGGGTACCGGTGCTTATGCGGTGCTgggaaaaattggggaaagcggggccg > 1:792475/1‑226 (MQ=255) cggcggTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAAAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCa > 1:450160/1‑251 (MQ=255) ggTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGGACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGa > 1:761968/1‑251 (MQ=255) aCGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGA‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACg < 1:329111/250‑1 (MQ=255) ggCAACAAGTGGTGAATTTCCCGCAGGCGGTGACCGCCCCGG‑AAATGAATGTACGTATTAACCCGCACTTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAAcc < 1:426258/122‑1 (MQ=255) aacaaGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTAGGTACCGATGCTTTTGCGCTGCTGGAACAAATGGCGGAACGCGTGCCCGGCGGAGCCAATGACGGGATCCCGAtt > 1:798588/1‑251 (MQ=255) tggtgAATTTACCGCAGGCGGTGCCCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTcc < 1:198132/251‑1 (MQ=255) attttcccGCGGGCGGTGACCG‑CCCGGAAAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGc < 1:291063/245‑1 (MQ=255) aaTTTACCGCGGGCGGTGACCGCCCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGc < 1:213680/251‑1 (MQ=255) ggCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAAc > 1:154560/1‑152 (MQ=255) ggCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTa > 1:376331/1‑251 (MQ=255) ggCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTa > 1:342622/1‑251 (MQ=255) tGACCGACCCGG‑AAATGAATGTACGTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGc < 1:205558/250‑1 (MQ=255) tGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGc < 1:90989/250‑1 (MQ=255) accCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTAt > 1:710702/1‑251 (MQ=255) cacccggtgtgccctgTATTAACCCGCACGTTACCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCAc < 1:863002/236‑1 (MQ=255) gaaaaTGAATGTACGTATTACCCCGCACTTTTCCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCAc < 1:11557/248‑1 (MQ=255) aaaaTGAATGTACGTATTACCCCGCACGTTACCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCAcgc < 1:680648/250‑1 (MQ=255) aaataagagtacgttgtagcccacACGTTATGCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGTTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCACCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCAcgc < 1:487801/227‑1 (MQ=255) tgaatgTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCa > 1:497676/1‑251 (MQ=255) gaatgTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGc > 1:355027/1‑150 (MQ=255) gaatgTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGcc > 1:263378/1‑235 (MQ=255) aatgTACGTATTACCCCGCACTTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCAc < 1:597189/250‑1 (MQ=255) gTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGt > 1:386259/1‑250 (MQ=255) gTATTACCCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCa < 1:103131/69‑1 (MQ=255) gTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTACCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCAc > 1:436965/1‑243 (MQ=255) cGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGAttt > 1:418610/1‑188 (MQ=255) ttaccccGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTAt < 1:576715/246‑1 (MQ=255) aTCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGTTGCATTTTGCGAAGAAGAAAAGAGACTGGCGGGAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGa < 1:340535/251‑1 (MQ=255) aTCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGa > 1:410244/1‑251 (MQ=255) aTCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGa < 1:358132/251‑1 (MQ=255) ccgTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGa > 1:382700/1‑251 (MQ=255) cgTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCCTTTTAAAGCCTGGTATCAcgc > 1:605129/1‑214 (MQ=255) cgTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGa > 1:730844/1‑250 (MQ=255) cgTTAGTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGAc < 1:194982/251‑1 (MQ=255) gTACAGGCGGAATCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGACAAATg > 1:87155/1‑251 (MQ=255) tCCATTAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTAt < 1:467781/224‑1 (MQ=255) ttAGCTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGACAAATGCAACAAAGCCGtatta < 1:168197/251‑1 (MQ=255) cTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGACAAATGCAACAAAGCCGTATTATTc < 1:195689/250‑1 (MQ=255) cTTTTTCACCGGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGACAAATGCAACAAAGCCGTATTATTc < 1:483050/250‑1 (MQ=255) | AAGTTGCACAACAAACCGGTTT‑AATACAGGGCACACCGGTTGTGGTCGGCGGCGGGGACGTGCAGTTAGGTTGTA‑TTGGTTTAGGCGTCACCGAGCCCGCTCAAGCGGCAGTTATCGGCGGTACGTTCTGGCAACAAGTGGTGAATTTACCGCAGGCGGTGACCGACCCGG‑AAATGAATGTACGTATTAACCCGCACGTTATCCCGCCGTTAGTACAGGCGGAATCCATTAGCTTTTTCACCAGATTAACCATGCGCTGGTTCCGTGATGCATTTTGCGAAGAAGAAAAGAGACTGGCGGAAAAACTGGGTACCGATGCTTATGCGTTGCTGGAACAAATGGCGGAACGCGTGCCCGTCGGCGCCAATGACGTGATCCCGATTTTCTCCGATGCCATGCATTTTAAAGCCTGGTATCACGCCGCACCGTCTTTTATTAATCTCTCTATCGATCCCGACAAATGCAACAAAGCCGTATTATTC > 1/1521795‑1522276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |