Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,520,992	T→C	*299Q (TAG→CAG)	unknown →	LsrR, transcriptional repressor of lsr operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,520,992	0	T	C	100.0%	193.4 / NA	64	*299Q (TAG→CAG)	unknown	LsrR, transcriptional repressor of lsr operon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/36);  total (28/36)

AAAAAGGACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTATAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCAAATCGCCGCCGCTCAACGGGAATGGACGCACTTATCCGA  >  1/1520747‑1521237                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aaaaaGGACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:534755/251‑1 (MQ=255) aaaaaGGACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:636305/251‑1 (MQ=255) aaaaaGGACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:347912/251‑1 (MQ=255) aaaaaGGACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:818136/251‑1 (MQ=255)       gACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCa                                                                                                                                                                                                                                             <  1:559702/250‑1 (MQ=255)       gACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCa                                                                                                                                                                                                                                             <  1:324413/250‑1 (MQ=255)       gACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCa                                                                                                                                                                                                                                             <  1:250413/250‑1 (MQ=255)             cGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAAt                                                                                                                                                                                                                                      <  1:848729/251‑1 (MQ=255)                  cTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCAtt                                                                                                                                                                                                                                 >  1:792390/1‑251 (MQ=255)                    gctccgctccGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTgg                                                                                                                                                                                                                               >  1:603442/1‑251 (MQ=255)                          ctccgGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTc                                                                                                                                                                                                                                    >  1:515632/1‑240 (MQ=255)                            ccgGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGgaa                                                                                                                                                                                                                       <  1:558844/251‑1 (MQ=255)                              ggTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  >  1:70927/1‑222 (MQ=255)                              ggTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAAt                                                                                                                                                                                                                                      <  1:705468/234‑1 (MQ=255)                                                                 gAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGttt                                                                                                                                                                                  >  1:805426/1‑251 (MQ=255)                                                                  aaTCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTaa                                                                                                                                                                                                                                       >  1:129190/1‑197 (MQ=255)                                                                        ggCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGttt                                                                                                                                                                                  >  1:750799/1‑244 (MQ=255)                                                                            cAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGgtg                                                                                                                                                                       >  1:247860/1‑251 (MQ=255)                                                                               tGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAg                                                                                                                                                                    <  1:291883/251‑1 (MQ=255)                                                                                     atatTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCggg                                                                                                                                                                >  1:92993/1‑249 (MQ=255)                                                                                            cGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATAtt                                                                                                                                                                                                                                         >  1:578555/1‑169 (MQ=255)                                                                                               ttATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCTGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTttcttc                                                                                                                                                    <  1:23228/251‑1 (MQ=255)                                                                                                 aTTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTcc                                                                                                                                                   >  1:796406/1‑250 (MQ=255)                                                                                                  tttCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:217844/154‑1 (MQ=255)                                                                                                         cGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  >  1:377775/1‑147 (MQ=255)                                                                                                                   gCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:457188/137‑1 (MQ=255)                                                                                                                    cAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAaat                                                                                                                                <  1:293975/250‑1 (MQ=255)                                                                                                                      gTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTc                                                                                                                                                                                                                                    <  1:471710/148‑1 (MQ=255)                                                                                                                      gTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAg                                                                                                                                                                            >  1:331744/1‑204 (MQ=255)                                                                                                                          aCAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTTGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAAc                                                                                                                         <  1:440012/251‑1 (MQ=255)                                                                                                                           cAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGt                                                                                                                                                                                                                                                  <  1:578091/129‑1 (MQ=255)                                                                                                                           cAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAatgatg                                                                                                                                                                                                  >  1:259941/1‑177 (MQ=255)                                                                                                                                 gATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCata                                                                                                                                                                                                                                           >  1:109071/1‑130 (MQ=255)                                                                                                                                 gATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCgg                                                                                                                                                                 >  1:605802/1‑204 (MQ=255)                                                                                                                                    atGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGgcgc                                                                                                                                                                                           <  1:673479/175‑1 (MQ=255)                                                                                                                                       ccGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCa                                                                                                                    <  1:551909/243‑1 (MQ=255)                                                                                                                                          ttACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAg                                                                                                                                                                                <  1:52967/180‑1 (MQ=255)                                                                                                                                          ttACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTAcc                                                                                                         <  1:579933/251‑1 (MQ=255)                                                                                                                                          ttACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTAcc                                                                                                         <  1:12868/251‑1 (MQ=255)                                                                                                                                              aTGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGAt                                                                                                     <  1:618854/251‑1 (MQ=255)                                                                                                                                                 aGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATgg                                                                                                   <  1:288026/250‑1 (MQ=255)                                                                                                                                                   gAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAAc                                                                                                                         >  1:3508/1‑226 (MQ=255)                                                                                                                                                   gAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTg                                                                                                               <  1:423114/236‑1 (MQ=255)                                                                                                                                                   gAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTg                                                                                                       <  1:539983/244‑1 (MQ=255)                                                                                                                                                     aTTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGCGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTa                                                                                              >  1:475377/1‑251 (MQ=255)                                                                                                                                                        aaTCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTc                                                                                                                                                                                                                                    <  1:779528/114‑1 (MQ=255)                                                                                                                                                                   cGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTa                                                                                                                                                                              <  1:7000/157‑1 (MQ=255)                                                                                                                                                                      tAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAg                                                                             <  1:344822/251‑1 (MQ=255)                                                                                                                                                                                             cATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGAt                                                       <  1:859181/250‑1 (MQ=255)                                                                                                                                                                                                 ttATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGa                                                  >  1:473337/1‑251 (MQ=255)                                                                                                                                                                                                  tATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGc                                                                                                                 >  1:798850/1‑187 (MQ=255)                                                                                                                                                                                                           cGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGtt                                                                         >  1:29371/1‑218 (MQ=255)                                                                                                                                                                                                           cGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTAttttt                                                          >  1:413582/1‑233 (MQ=255)                                                                                                                                                                                                             ccGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCaaa                                       <  1:754921/250‑1 (MQ=255)                                                                                                                                                                                                                gtggtgAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAg                                                                                                                                                                                >  1:237833/1‑110 (MQ=255)                                                                                                                                                                                                                  ggtgAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTg                                                                                                       <  1:95437/181‑1 (MQ=255)                                                                                                                                                                                                                  ggtgAAGACAAAGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAACGAACAATCAAGCTGTTTACCTGATGGCATTAGAt                                                                                           >  1:665190/1‑193 (MQ=255)                                                                                                                                                                                                                            aaGTCGAAGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCAAATCGCCGCCGCTCAACg                       >  1:236415/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaGCGATTTTAAGCGCATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCAAATCGCCGCCGCTCAACGGGAATg                 <  1:549988/251‑1 (MQ=255)                                                                                                                                                                                                                                              gcgcATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAg                                                                             >  1:337164/1‑179 (MQ=255)                                                                                                                                                                                                                                               cgcATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCAAATCGCCGCCGCTCAACGGGAATGGACGCACTTATcc    <  1:854800/251‑1 (MQ=255)                                                                                                                                                                                                                                                gcATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTAcc                                                                                                         <  1:524621/149‑1 (MQ=255)                                                                                                                                                                                                                                                 cATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAg                                                                                                                                                                                >  1:388946/1‑77 (MQ=255)                                                                                                                                                                                                                                                 cATTACAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCAAATCGCCGCCGCTCAACGGGAATGGACGCACTTATCCGa  >  1:92454/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AAAAAGGACAGGCGACACTGCTCCGCTCCGGTTATATCACCGAAGAAAAACAACAAATTATTCGGAATCAGGGCGCAGTGGGCGATATTCTCGGTTATTTCATGCGTAAAGACGGCAGTTTACAACCGGATATGCCGTTACATGAGGAATTAATCAGCGTTTCGTTAGAAAATTTGAAAAAAATTCCGCATGTTATCGGTGTCGCCGGTGGTGAAGACAAAGTCGAAGCGATTTTAAGCGCATTATAGGGTAATCATATTAATTCATTGGTTACGGAAGAAAACACGGCTCGAATGATGTTGGCGCAGTTGGTTTAGTAAGCGGTGTAGCGGGCTTATTTTCTTCCCAAACAAAGAGGAGAAAATAATGAACAATCAAGCTGTTTACCTGATGGCATTAGATGCCGGCACAGGAAGTGTTCGGGCGGTTATTTTTGATTTAGACGGTAATCAAATCGCCGCCGCTCAACGGGAATGGACGCACTTATCCGA  >  1/1520747‑1521237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑