Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,519,813	G→A	A14A (GCC→GCT)	unknown ←	Autoinducer 2 (AI‑2) ABC transporter, dimeric ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,519,813	0	G	A	100.0%	139.2 / NA	47	A14A (GCC→GCT)	unknown	Autoinducer 2 (AI‑2) ABC transporter, dimeric ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/29);  total (18/29)

CGGTTCCTGCGGCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGGGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTCAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATA  >  1/1519572‑1520044                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        cGGTTCCTGCGGCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGatat                                                                                                                                                                                                                                 <  1:835907/250‑1 (MQ=255)   gTTCCTGCGGCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTaa                                                                                                                                                                                                                              >  1:527610/1‑251 (MQ=255)     tCCTGCGGCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGc                                                                                                                                                                                                                            <  1:284551/251‑1 (MQ=255)       cTGCGGCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCAt                                                                                                                                                                                                                          <  1:172804/251‑1 (MQ=255)          cGGCACCAAATAAATACCTAGCTGAGGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTCCATCAGCATGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCAtttt                                                                                                                                                                                                                       <  1:88713/251‑1 (MQ=255)            gCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTgg                                                                                                                                                                                                                     >  1:753074/1‑251 (MQ=255)                  aataaataCCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGTGGATATTAAGCATTTTGGATCTGa                                                                                                                                                                                                               >  1:643449/1‑251 (MQ=255)                         aCCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGaaa                                                                                                                                                                                                             <  1:565714/246‑1 (MQ=255)                         aCCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGa                                                                                                                                                                                                        >  1:484737/1‑251 (MQ=255)                          ccTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAg                                                                                                                                                                                                       <  1:435380/251‑1 (MQ=255)                          ccTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAg                                                                                                                                                                                                       <  1:589247/251‑1 (MQ=255)                               cTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGc                                                                                                                                                                                                                            >  1:858088/1‑225 (MQ=255)                                  aTGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCAt                                                                                                                                                                                                <  1:724333/250‑1 (MQ=255)                                      gCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTa                                                                                                                                                                                                                               <  1:530760/215‑1 (MQ=255)                                           aTTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTAcc                                                                                                                                                                                        >  1:338517/1‑249 (MQ=255)                                            ttCGGAGAAAGCTTATTTGGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGc                                                                                                                                                                                     <  1:489764/251‑1 (MQ=255)                                                gagaAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAg                                                                                                                                                                                                       <  1:827772/229‑1 (MQ=255)                                                  gaAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATa                                                                                                                                                                               >  1:759501/1‑251 (MQ=255)                                                   aaaGCTTATTTTGTCGCGCCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAg                                                                                                                                                                              <  1:203286/251‑1 (MQ=255)                                                     aGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGaa                                                                                                                                                                            >  1:770849/1‑251 (MQ=255)                                                           ttttGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACa                                                                                                                                                                      <  1:837598/251‑1 (MQ=255)                                                               gTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATg                                                                                                                                                                  >  1:284326/1‑251 (MQ=255)                                                                tCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATg                                                                                                                                                                  <  1:32228/250‑1 (MQ=255)                                                                    cccccGAACCTCCAGTTCTGCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCTTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTAttt                                                                                                                                                             <  1:314817/250‑1 (MQ=255)                                                                          aaCCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACa                                                                                                                                                                      <  1:899345/236‑1 (MQ=255)                                                                          aaCCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTaaaaaa                                                                                                                                                       >  1:241813/1‑251 (MQ=255)                                                                            ccTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGtt                                                                                                                                                                                                     <  1:860682/203‑1 (MQ=255)                                                                             cTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACa                                                                                                                                                                      <  1:573243/233‑1 (MQ=255)                                                                               ccAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACAt                                                                                                                                                  >  1:29688/1‑251 (MQ=255)                                                                                            aTCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGt                                                                                                                                                                 >  1:541616/1‑223 (MQ=255)                                                                                                      tGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGaa                                                                                                                           <  1:741126/251‑1 (MQ=255)                                                                                                       gAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGaa                                                                                                                           >  1:880307/1‑250 (MQ=255)                                                                                                                    aaTTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTc                                                                                                                                <  1:312919/232‑1 (MQ=255)                                                                                                                                     aTTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACaa                                                                                             <  1:659379/250‑1 (MQ=255)                                                                                                                                       ttCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATc                                                                                          <  1:770588/251‑1 (MQ=255)                                                                                                                                            cgccccgTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGAt                                                                                     <  1:486868/251‑1 (MQ=255)                                                                                                                                              ccccgTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTc                                                                                                                                <  1:822660/206‑1 (MQ=255)                                                                                                                                                   ttACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTAcc                                                                                                                                                                                        >  1:574372/1‑145 (MQ=255)                                                                                                                                                      ccgccgAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGAt                                                                                <  1:348258/246‑1 (MQ=255)                                                                                                                                                                gtgcgtgcACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGc                                                                  >  1:837841/1‑250 (MQ=255)                                                                                                                                                                 tgcgtgcACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAgg                                                                                                                             >  1:151273/1‑190 (MQ=255)                                                                                                                                                                       cACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACa                                                                                                                                                                      <  1:460162/143‑1 (MQ=255)                                                                                                                                                                       cACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAAtt                                                          <  1:328985/251‑1 (MQ=255)                                                                                                                                                                                 gCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTaaaaa                                                                      >  1:391742/1‑229 (MQ=255)                                                                                                                                                                                               aTCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGctc                                                                                                                                                                                                                  <  1:551551/75‑1 (MQ=255)                                                                                                                                                                                                     ggCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGAt                                                                                >  1:208877/1‑199 (MQ=255)                                                                                                                                                                                                                               aCGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGata  <  1:840746/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        CGGTTCCTGCGGCACCAAATAAATACCTAGCTGATGGGCTTTATTCGGAGAAAGCTTATTTTGTCGCACCCCGAACCTCCAGTTCTCCGCTATCTTGCGGCTGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGGGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTCAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATA  >  1/1519572‑1520044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑