Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 213,713 T→C Y6Y (TAT→TAC)  unknown → L‑threonate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1213,7130TC100.0% 124.6 / NA 42Y6Y (TAT→TAC) unknownL‑threonate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/25);  total (17/25)

TTTGTCGCTCTGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATATTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAG  >  1/213482‑213954
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
tttGTCGCTCTGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGc                                                                                                                                                                                                                                <  1:854603/251‑1 (MQ=255)
         ctGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCaa                                                                                                                                                                                                                       <  1:891994/251‑1 (MQ=255)
           gCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGCTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATg                                                                                                                                                                                                                     >  1:885140/1‑251 (MQ=255)
                     tAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATggggg                                                                                                                                                                                                           <  1:117277/251‑1 (MQ=255)
                                aaaaaTTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCt                                                                                                                                                                                                <  1:444166/251‑1 (MQ=255)
                                                      ctTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGc                                                                                                                                                                                                                                           <  1:533396/186‑1 (MQ=255)
                                                               tATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGgtg                                                                                                                                                                 <  1:678377/251‑1 (MQ=255)
                                                                     gttgttATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAagag                                                                                                                                                                                      >  1:100388/1‑224 (MQ=255)
                                                                             tCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATaa                                                                                                                                                                                         <  1:619806/213‑1 (MQ=255)
                                                                                                             aaataaTAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttg                                                                                                                                                                                                                                      <  1:206348/136‑1 (MQ=255)
                                                                                                                     atGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCt                                                                                                                                                                                                <  1:501345/166‑1 (MQ=255)
                                                                                                                     atGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCaa                                                                                                           >  1:568026/1‑251 (MQ=255)
                                                                                                                       gTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttgttg                                                                                                                                                                                                                                   >  1:336208/1‑129 (MQ=255)
                                                                                                                       gTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAAccc                                                                                                                                                    <  1:587504/208‑1 (MQ=255)
                                                                                                                        ttAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAAcc                                                                                                         <  1:561093/250‑1 (MQ=255)
                                                                                                                             ttttGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGc                                                                                                                                                                                                                         <  1:13931/133‑1 (MQ=255)
                                                                                                                                    aCATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGtat                                                                                                                                                                                           >  1:847543/1‑156 (MQ=255)
                                                                                                                                    aCATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGc                                                                                                                                              <  1:791441/201‑1 (MQ=255)
                                                                                                                                         gCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGgtg                                                                                                                                                                 <  1:855122/177‑1 (MQ=255)
                                                                                                                                         gCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGAt                                                                                       >  1:894963/1‑251 (MQ=255)
                                                                                                                                                              gATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGtat                                                                                                                                                                                           >  1:806521/1‑130 (MQ=255)
                                                                                                                                                                    gCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttg                                                                                                                                                                                                                                      <  1:874443/81‑1 (MQ=255)
                                                                                                                                                                    gCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTttg                                                            >  1:731297/1‑251 (MQ=255)
                                                                                                                                                                       gAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTAttgt                                                                                                                                                                                                                                     >  1:180119/1‑79 (MQ=255)
                                                                                                                                                                       gAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGtat                                                                                                                                                                                           >  1:901712/1‑121 (MQ=255)
                                                                                                                                                                                ccGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTgtagta                                                >  1:897904/1‑251 (MQ=255)
                                                                                                                                                                                ccGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTgtagta                                                <  1:543238/251‑1 (MQ=255)
                                                                                                                                                                                ccGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTgtagta                                                >  1:302992/1‑251 (MQ=255)
                                                                                                                                                                                  gTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAagag                                                                                                                                                                                      <  1:124031/115‑1 (MQ=255)
                                                                                                                                                                                          aacaaACTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGc                                      <  1:192310/251‑1 (MQ=255)
                                                                                                                                                                                              aaCTTTATTATTTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCa                                                                                                                                                                                                                 <  1:781939/76‑1 (MQ=255)
                                                                                                                                                                                                    attattTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTttgttg                                                         <  1:335062/222‑1 (MQ=255)
                                                                                                                                                                                                       attTGGAGAAATACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAAcc                                                                                                         >  1:571820/1‑171 (MQ=255)
                                                                                                                                                                                                                 aTACCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGTCCTATGgtgt                                                                                                                                                                >  1:593947/1‑106 (MQ=255)
                                                                                                                                                                                                                   aCCATGACAAACAAATCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCt                                                                                                                                                                                                <  1:624210/72‑1 (MQ=255)
                                                                                                                                                                                                                          caaacaaaTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGc                                      <  1:223150/219‑1 (MQ=255)
                                                                                                                                                                                                                              caaaTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAGCCAGCCAAAGTGCGGTCGATTTTACGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAg  >  1:443710/1‑251 (MQ=255)
                                                                                                                                                                                                                              caaaTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAg  >  1:504922/1‑251 (MQ=255)
                                                                                                                                                                                                                                 aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATgg                                                                                                                                                                   >  1:695820/1‑87 (MQ=255)
                                                                                                                                                                                                                                 aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAAt                                             <  1:52247/205‑1 (MQ=255)
                                                                                                                                                                                                                                 aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTc                                 <  1:349380/217‑1 (MQ=255)
                                                                                                                                                                                                                                 aTCATACTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCgg                          <  1:865164/224‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
TTTGTCGCTCTGCAGGAATCATAACCCCTCCAAAAAATTTTCGTCATTATATCTCTTTTTGTTTATTTTGTTGTTATTCCATTCATTTTTCACATAAAATAACAAAAATAAATAATAATGTTAAATTTTGTGACATTGCTCACGTTTTTTTATTTTAAGATCCGGCAGAATGGATACCGTTAGATCAACAAACTTTATTATTTGGAGAAATACCATGACAAACAAATCATATTCTGTTGCTATTGTTGGGCTGGGTGCAATGGGCATGGGGGCGGCTCAGTCTTGTATAAGAGCCGGATTGACGACCTATGGTGTAGATCTTAACCCAAAAGCACTTGAAAAATTAAAAACTGATGGCGCACACGCAACCAGCCAAAGTGCGGTCGATTTTGCGGATAAATTAGATGCAGTTTTGTTGCTTGTAGTAAATGCTGTGCAAGTCAATTCGGTGCTATTTGGTGAAAATGGTTTAG  >  1/213482‑213954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑