Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,516,654	A→G	C211R (TGC→CGC)	unknown ←	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,516,654	0	A	G	100.0%	135.1 / NA	48	C211R (TGC→CGC)	unknown	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/9);  total (39/9)

GGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCAACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACC  >  1/1516412‑1516894                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gggAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCg                                                                                                                                                                                                                                          >  1:214204/1‑251 (MQ=255)  ggAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCgg                                                                                                                                                                                                                                         >  1:869812/1‑251 (MQ=255)               tttGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTaa                                                                                                                                                                                                                            >  1:366184/1‑251 (MQ=255)                                     aCCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGa                                                                                                                                                                                                                       >  1:637359/1‑234 (MQ=255)                                     aCCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGc                                                                                                                                                                                                       >  1:836688/1‑250 (MQ=255)                                      ccGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTaa                                                                                                                                                                                                                            >  1:553381/1‑228 (MQ=255)                                             gCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTaaa                                                                                                                                                                                              >  1:559797/1‑251 (MQ=255)                                             gCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTaaa                                                                                                                                                                                              >  1:841588/1‑251 (MQ=255)                                             gCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTaaa                                                                                                                                                                                              >  1:342599/1‑251 (MQ=255)                                                    ggcAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCCGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATCATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTg                                                                                                                                                                                        >  1:770153/1‑250 (MQ=255)                                                                gCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATCATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGa                                                                                                                                                                                                                       >  1:438270/1‑207 (MQ=255)                                                                gCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTaa                                                                                                                                                                                                                            >  1:500536/1‑202 (MQ=255)                                                                gCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGAtgg                                                                                                                                                                           >  1:88112/1‑251 (MQ=255)                                                                                             tatTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTaa                                                                                                                                                                                                                            >  1:103609/1‑173 (MQ=255)                                                                                                       gCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTaa                                                                                                                                                                                                                            >  1:480483/1‑163 (MQ=255)                                                                                                       gCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATCTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGAGCTTCTGCCGATGGTGGTTTTGTGCACTAATAACCAAAAGAACAGCGTCATTa                                                                                                                                    >  1:733987/1‑251 (MQ=255)                                                                                                            acacCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGa                                                                                                                               >  1:127886/1‑251 (MQ=255)                                                                                                                      ggTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGa                                                                                                                     >  1:842483/1‑251 (MQ=255)                                                                                                                                     cagcagGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATg                                                                                                       >  1:390921/1‑250 (MQ=255)                                                                                                                                       gcagGGACCTGCCTAAGTCGGAGCGGGCGAAACCAAAAAAAGCCCCCAGCACCACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATtaa                                                                                                                                                           <  1:458734/196‑1 (MQ=255)                                                                                                                                       gcagGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCg                                                                                                    >  1:478479/1‑251 (MQ=255)                                                                                                                                          gggAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAAt                                                                                                 >  1:325123/1‑251 (MQ=255)                                                                                                                                                 gCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGa                                                                                                                                                                                                                       >  1:295691/1‑126 (MQ=255)                                                                                                                                                 gCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGttt                                                                                          >  1:152508/1‑251 (MQ=255)                                                                                                                                                           ggggggcgaaaactaaaaaaacaccccAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGa                                                                                 <  1:183787/225‑1 (MQ=255)                                                                                                                                                                    gAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                       >  1:241243/1‑251 (MQ=255)                                                                                                                                                                    aaacaaaaaaaagccacaaGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                       <  1:586771/233‑1 (MQ=255)                                                                                                                                                                          aaaaaaaacaccacaaaCACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATc                                                                 <  1:369293/236‑1 (MQ=255)                                                                                                                                                                                               gCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTaa                                                                                                                                                                                                                            >  1:114507/1‑75 (MQ=255)                                                                                                                                                                                                     aaCGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAAcc                                      <  1:41819/251‑1 (MQ=255)                                                                                                                                                                                                       cGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATtaa                                                                                                                                                           <  1:505807/132‑1 (MQ=255)                                                                                                                                                                                                       cGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAAc                                       >  1:897291/1‑248 (MQ=255)                                                                                                                                                                                                       cGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAAc                                       >  1:854341/1‑248 (MQ=255)                                                                                                                                                                                                                  cGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCa                         >  1:730188/1‑251 (MQ=255)                                                                                                                                                                                                                    ccAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCaaa                       >  1:598171/1‑251 (MQ=255)                                                                                                                                                                                                                     cAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCaaaa                      >  1:606894/1‑251 (MQ=255)                                                                                                                                                                                                                           aGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGa                 >  1:467420/1‑250 (MQ=255)                                                                                                                                                                                                                             gAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                       >  1:205112/1‑194 (MQ=255)                                                                                                                                                                                                                                   aTCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAg                                                          >  1:225921/1‑201 (MQ=255)                                                                                                                                                                                                                                     cAATAATAATGTGTGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACg      >  1:264992/1‑251 (MQ=255)                                                                                                                                                                                                                                     cAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCAt                                          >  1:276386/1‑215 (MQ=255)                                                                                                                                                                                                                                     cAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAAcc                                      >  1:279001/1‑219 (MQ=255)                                                                                                                                                                                                                                     cAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACg      >  1:283550/1‑251 (MQ=255)                                                                                                                                                                                                                                      aataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGc     <  1:515863/251‑1 (MQ=255)                                                                                                                                                                                                                                       ataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATaa                                                                                               <  1:540100/160‑1 (MQ=255)                                                                                                                                                                                                                                       ataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGAt                                                  >  1:829491/1‑205 (MQ=255)                                                                                                                                                                                                                                       ataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGca    >  1:505138/1‑251 (MQ=255)                                                                                                                                                                                                                                         aataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGcacc  <  1:483812/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCAACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACC  >  1/1516412‑1516894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑