Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,489,207	A→G	A108A (GCT→GCC)	unknown ←	Protease HtpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,489,207	0	A	G	100.0%	234.3 / NA	81	A108A (GCT→GCC)	unknown	Protease HtpX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/52);  total (29/52)

ACCGGAATTGCTTGAACTTTCACTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTAGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAACAGTGCCGCGAAAATCATTAACCCGGACGCGTCACG  >  1/1488964‑1489443                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               aCCGGAATTGCTTGAACTTTCACTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAgg                                                                                                                                                                                                                                         <  1:805228/251‑1 (MQ=255)   cGGAATTGCTTGAACTTTCACTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCg                                                                                                                                                                                                                                       <  1:760190/251‑1 (MQ=255)               aaCTTTCACTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCg                                                                                                                                                                                                                           >  1:850822/1‑251 (MQ=255)                     cACTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGa                                                                                                                                                                                                                     <  1:77335/251‑1 (MQ=255)                       cTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATa                                                                                                                                                                                                                   >  1:599019/1‑251 (MQ=255)                        tGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATaa                                                                                                                                                                                                                  <  1:655928/251‑1 (MQ=255)                        tGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATaa                                                                                                                                                                                                                  <  1:590015/251‑1 (MQ=255)                             tGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGc                                                                                                                                                                                                             <  1:368065/251‑1 (MQ=255)                              gATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCa                                                                                                                                                                                                                                             <  1:746318/218‑1 (MQ=255)                              gATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAAt                                                                                                                                                                                                                >  1:776063/1‑247 (MQ=255)                              gATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGc                                                                                                                                                                                                             >  1:229175/1‑250 (MQ=255)                               aTTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGcc                                                                                                                                                                                                            <  1:752031/250‑1 (MQ=255)                                       aaTTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAggg                                                                                                                                                                                                   <  1:246539/251‑1 (MQ=255)                                        aTTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGgc                                                                                                                                                                                                  <  1:109472/251‑1 (MQ=255)                                          tGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGgcgc                                                                                                                                                                                                <  1:352135/251‑1 (MQ=255)                                                   caTTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCCCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCg                                                                                                                                                                                       >  1:698991/1‑251 (MQ=255)                                                      tCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTTCTTTCGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCa                                                                                                                                                                                    <  1:756073/251‑1 (MQ=255)                                                         cgATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATaa                                                                                                                                                                                                                  <  1:107925/218‑1 (MQ=255)                                                           aTTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATaa                                                                                                                                                                                                                  >  1:847079/1‑216 (MQ=255)                                                                   cgATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑g                                                                                                                                                                     <  1:398178/251‑1 (MQ=255)                                                                     aTAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCATCGGAATGATAAATAGCCACTTCAGTGCGCGGTAAACCGGCACGTTCTGCTGGC‑‑Gaa                                                                                                                                                                   >  1:853875/1‑251 (MQ=255)                                                                     aTAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑Gaa                                                                                                                                                                   >  1:540545/1‑251 (MQ=255)                                                                          aaaaTGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATacac                                                                                                                                                              <  1:765159/251‑1 (MQ=255)                                                                             aTGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGt                                                                                                                                                           <  1:614150/251‑1 (MQ=255)                                                                                   aaGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATccac                                                                                                                                                     <  1:743275/251‑1 (MQ=255)                                                                                     ggTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATccacca                                                                                                                                                   >  1:311268/1‑251 (MQ=255)                                                                                      gTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATaa                                                                                                                                                                                                                  >  1:730061/1‑189 (MQ=255)                                                                                      gTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCATGTTCTGCTTGC‑‑GAATACACGGTATccacc                                                                                                                                                    <  1:37384/249‑1 (MQ=255)                                                                                      gTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATccacc                                                                                                                                                    <  1:329268/249‑1 (MQ=255)                                                                                      gTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATccacc                                                                                                                                                    <  1:459287/249‑1 (MQ=255)                                                                                              cACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTGCTGCTTGC‑‑GAATACACGGTATCCACcaaccaac                                                                                                                                             <  1:636599/248‑1 (MQ=255)                                                                                               aCGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTc                                                                                                                                         <  1:577148/251‑1 (MQ=255)                                                                                                       cAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCAt                                                                                                                                 >  1:796231/1‑251 (MQ=255)                                                                                                            aTGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTAt                                                                                                                                                         >  1:744687/1‑222 (MQ=255)                                                                                                                 aTCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGAc                                                                                                                                                                                                                                  <  1:28220/146‑1 (MQ=255)                                                                                                                 aTCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCt                                                                                                                                                                           >  1:624581/1‑201 (MQ=255)                                                                                                                 aTCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGgttg                                                                                                                       >  1:531008/1‑251 (MQ=255)                                                                                                                       aCCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGaa                                                                                                                  <  1:567104/250‑1 (MQ=255)                                                                                                                       aCCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGaa                                                                                                                  <  1:385949/250‑1 (MQ=255)                                                                                                                          aTGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAAt                                                                                                                 >  1:873381/1‑248 (MQ=255)                                                                                                                                   tttttAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGgc                                                                                                                                                                                                  <  1:801626/160‑1 (MQ=255)                                                                                                                                   tttttAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCAc                                                                                                      <  1:869977/250‑1 (MQ=255)                                                                                                                                    ttttAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCAc                                                                                                                                                                                   >  1:503941/1‑174 (MQ=255)                                                                                                                                    ttttAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGCGAGAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCa                                                                                                       >  1:532691/1‑250 (MQ=255)                                                                                                                                       tAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCAc                                                                                                      <  1:805216/246‑1 (MQ=255)                                                                                                                                             ggCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATaa                                                                                                                                                                                                                  <  1:226082/134‑1 (MQ=255)                                                                                                                                                   cTTCATGCGCCAATACCGCTTCTGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCaccaacc                                                                                                                                                <  1:451198/192‑1 (MQ=255)                                                                                                                                                        tGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGt                                                                                <  1:381436/251‑1 (MQ=255)                                                                                                                                                          cgcCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATccac                                                                                                                                                     <  1:198151/180‑1 (MQ=255)                                                                                                                                                            ccAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTa                                                                            >  1:868854/1‑251 (MQ=255)                                                                                                                                                               aTACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGAc                                                                         <  1:198225/251‑1 (MQ=255)                                                                                                                                                               aTACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGAc                                                                         <  1:243771/251‑1 (MQ=255)                                                                                                                                                               aTACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGAc                                                                         <  1:363044/251‑1 (MQ=255)                                                                                                                                                               aTACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGAc                                                                         <  1:868525/251‑1 (MQ=255)                                                                                                                                                                tACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGt                                                                                >  1:827234/1‑243 (MQ=255)                                                                                                                                                                     cttccgcttcACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACataaa                                                                    <  1:181207/250‑1 (MQ=255)                                                                                                                                                                       tccgcttcACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACataaataa                                                                 <  1:299778/251‑1 (MQ=255)                                                                                                                                                                        ccgcttcACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGaa                                                                                                                  <  1:697599/201‑1 (MQ=255)                                                                                                                                                                         cgcttcACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAgg                                                               <  1:98275/251‑1 (MQ=255)                                                                                                                                                                             tcACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACTCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGAc                                                                         >  1:213382/1‑237 (MQ=255)                                                                                                                                                                                ccGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAatgat                                                        <  1:843037/251‑1 (MQ=255)                                                                                                                                                                                       tCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGAc                                                                         <  1:164406/227‑1 (MQ=255)                                                                                                                                                                                         aTTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAg                                                                <  1:769298/234‑1 (MQ=255)                                                                                                                                                                                            ttGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATccacc                                                                                                                                                    >  1:249859/1‑147 (MQ=255)                                                                                                                                                                                            ttGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCaaa                                            >  1:752458/1‑251 (MQ=255)                                                                                                                                                                                            ttGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCaaa                                            >  1:168349/1‑251 (MQ=255)                                                                                                                                                                                                      gCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGaa                                                                                                                  <  1:553397/171‑1 (MQ=255)                                                                                                                                                                                                      gCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCa                                                                                                               >  1:645797/1‑174 (MQ=255)                                                                                                                                                                                                      gCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACataaat                                                                   >  1:814849/1‑218 (MQ=255)                                                                                                                                                                                                      gCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAACAGt                                  >  1:77915/1‑251 (MQ=255)                                                                                                                                                                                                       ccccGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAAc                                     <  1:273531/247‑1 (MQ=255)                                                                                                                                                                                                       ccccGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAAc                                     <  1:796831/247‑1 (MQ=255)                                                                                                                                                                                                       ccccGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAAc                                     <  1:227681/247‑1 (MQ=255)                                                                                                                                                                                                          cGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAACAGTGCcg                              <  1:553991/251‑1 (MQ=255)                                                                                                                                                                                                           gTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTc                                                                                                                                         >  1:485023/1‑143 (MQ=255)                                                                                                                                                                                                            tACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAAc                                                   >  1:210324/1‑228 (MQ=255)                                                                                                                                                                                                             aCTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCgg                                                                                                                          <  1:171663/156‑1 (MQ=255)                                                                                                                                                                                                             aCTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAACAGTGCCGCGa                           <  1:191732/251‑1 (MQ=255)                                                                                                                                                                                                                                  attTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACcaaccaac                                                                                                                                             <  1:552475/116‑1 (MQ=255)                                                                                                                                                                                                                                  attTTTGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGAGCGCGGTAAACCGGCAc                                                                                                                                                                                   >  1:56315/1‑80 (MQ=255)                                                                                                                                                                                                                                      ttGCTCGCACCGGTGGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAACAGTGCCGCGAAAATCATTAACCCGGACGCGTCACg  <  1:544352/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ACCGGAATTGCTTGAACTTTCACTGCCCTGATTACGAGAATTGGCGACCACATTCGCGATTAAGCGCGATAAGAAAATGACGAAGGTATTCAGCACGCCTTGCAGTAATGCCATCGTGACCATGTCACCGTTTTTAATATGGCTCACTTCATGCGCCAATACCGCTTCCGCTTCACCGCGCGTCATTTTGTTTAACAGCCCCGTACTTACGGCAACCAGCGAATTATTTTTGCTCGCACCGGTAGCAAAGGCGTTGACATCAGCGGAATGATAAATAGCCACTTCAGGGCGCGGTAAACCGGCACGTTCTGCTTGC‑‑GAATACACGGTATCCACCAACCAACGTTCCGTTTCATTACGCGGTTGTTGAATCACTTCCGCACCGACGGCGCGAATCGCCATAGTTTTAGACATAAATAAGGAAATGATGGAACCGGCAAAACCGAACAGTGCCGCGAAAATCATTAACCCGGACGCGTCACG  >  1/1488964‑1489443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑