Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,470,247	G→A	P103P (CCG→CCA)	unknown →	Inner membrane transport permease YhhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,470,247	0	G	A	100.0%	244.5 / NA	84	P103P (CCG→CCA)	unknown	Inner membrane transport permease YhhJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/43);  total (41/43)

TTAATTATTTATATGTTCACCGTCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCGCCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCCACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAT  >  1/1470020‑1470483                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             ttAATTATTTATATGTTCACCGTCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACt                                                                                                                                                                                                                       <  1:425336/251‑1 (MQ=255)   aaTTATTTATATGTTCACCGTCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTg                                                                                                                                                                                                                     <  1:821913/251‑1 (MQ=255)       atttatATGTTCACCGTCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCggg                                                                                                                                                                                                                 >  1:572693/1‑251 (MQ=255)                ttCACCGTCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAAcc                                                                                                                                                                                                         <  1:753327/250‑1 (MQ=255)                       tCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGAt                                                                                                                                                                                                  <  1:182900/250‑1 (MQ=255)                          cTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGa                                                                                                                                                                                                                                   <  1:131128/214‑1 (MQ=255)                          cTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAg                                                                                                                                                                                              <  1:614649/251‑1 (MQ=255)                           tATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAAc                                                                                                                                                                                                          >  1:470623/1‑238 (MQ=255)                            aTTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGtt                                                                                                                                                                                            >  1:665131/1‑251 (MQ=255)                            aTTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGtt                                                                                                                                                                                            >  1:254559/1‑251 (MQ=255)                            aTTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGtt                                                                                                                                                                                            >  1:392908/1‑251 (MQ=255)                            aTTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGtt                                                                                                                                                                                            <  1:220667/251‑1 (MQ=255)                                                     ccTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACg                                                                                                                                                                   <  1:543173/251‑1 (MQ=255)                                                             gTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCaa                                                                                                                                                                >  1:116337/1‑246 (MQ=255)                                                                    aTGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGt                                                                                                                                                    <  1:485706/251‑1 (MQ=255)                                                                         tCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTc                                                                                                                                                                                                >  1:380706/1‑202 (MQ=255)                                                                         tCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGc                                                                                                                                               <  1:877413/251‑1 (MQ=255)                                                                         tCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGc                                                                                                                                               <  1:253649/251‑1 (MQ=255)                                                                           cGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCtt                                                                                                                                             <  1:448206/251‑1 (MQ=255)                                                                                ccATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTatat                                                                                                                                         <  1:397649/250‑1 (MQ=255)                                                                                    tgtgGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTca                                                                                                                                     >  1:553144/1‑250 (MQ=255)                                                                                     gtgGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATg                                                                                                                                                                                                                                    <  1:134716/154‑1 (MQ=255)                                                                                       ggATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTAc                                                                                                                                                                                                                        <  1:213406/164‑1 (MQ=255)                                                                                        gATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAAc                                                                                                                                                                                                                                 >  1:691469/1‑154 (MQ=255)                                                                                             cGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGCTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGt                                                                                                                                                    >  1:27837/1‑226 (MQ=255)                                                                                                       gCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAAt                                                                                                                 >  1:360976/1‑251 (MQ=255)                                                                                                            gTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCtt                                                                                                                                             >  1:400854/1‑218 (MQ=255)                                                                                                            gTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACg                                                                                                            >  1:612073/1‑251 (MQ=255)                                                                                                            gTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACg                                                                                                            >  1:798197/1‑251 (MQ=255)                                                                                                              cTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGAt                                                                                                          <  1:474196/251‑1 (MQ=255)                                                                                                                    gCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGa                                                                                                           >  1:368015/1‑244 (MQ=255)                                                                                                                    gCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGAtttt                                                                                                       >  1:433440/1‑248 (MQ=255)                                                                                                                         caacaaAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGtt                                                                                                                                                                                            >  1:563291/1‑158 (MQ=255)                                                                                                                         caacaaAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCAc                                                                                                 <  1:670326/249‑1 (MQ=255)                                                                                                                                gCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                         >  1:673450/1‑250 (MQ=255)                                                                                                                                 ccTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGc                                                                                        <  1:168582/250‑1 (MQ=255)                                                                                                                                      ttCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAAc                                                                                  <  1:131833/251‑1 (MQ=255)                                                                                                                                              aaCTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACt                                                                          <  1:844904/251‑1 (MQ=255)                                                                                                                                                  tCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCt                                                                                                                                              <  1:756991/179‑1 (MQ=255)                                                                                                                                                  tCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                         <  1:771660/232‑1 (MQ=255)                                                                                                                                                  tCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCa                                                                              >  1:239688/1‑243 (MQ=255)                                                                                                                                                  tCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGA‑‑ATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTatat                                                                                                                                         >  1:203712/1‑182 (MQ=255)                                                                                                                                                       aaaGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                 <  1:317951/251‑1 (MQ=255)                                                                                                                                                         aGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACgg                                                                                <  1:811215/234‑1 (MQ=255)                                                                                                                                                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTc                                                                     <  1:687576/244‑1 (MQ=255)                                                                                                                                                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAc                                                                  >  1:456730/1‑247 (MQ=255)                                                                                                                                                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACcca                                                               >  1:38761/1‑250 (MQ=255)                                                                                                                                                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACcca                                                               >  1:7486/1‑250 (MQ=255)                                                                                                                                                            gCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTatat                                                                                                                                         <  1:221882/174‑1 (MQ=255)                                                                                                                                                                aCAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCGCCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTaa                                                        <  1:157977/251‑1 (MQ=255)                                                                                                                                                                 cAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTaa                                                        <  1:737075/250‑1 (MQ=255)                                                                                                                                                                  aaTTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCGATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAAtg                                                      <  1:145630/251‑1 (MQ=255)                                                                                                                                                                    ttCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAc                                                                  <  1:133334/237‑1 (MQ=255)                                                                                                                                                                     tCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGc                                                   <  1:84154/251‑1 (MQ=255)                                                                                                                                                                       aTGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                 >  1:150973/1‑235 (MQ=255)                                                                                                                                                                               caGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAAcc                                          >  1:808992/1‑250 (MQ=255)                                                                                                                                                                                          ccTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                 >  1:379171/1‑216 (MQ=255)                                                                                                                                                                                             gATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTa                           >  1:819625/1‑251 (MQ=255)                                                                                                                                                                                               tGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAAc                         <  1:104594/251‑1 (MQ=255)                                                                                                                                                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTc                                                                         <  1:326392/200‑1 (MQ=255)                                                                                                                                                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTgg                      >  1:591876/1‑251 (MQ=255)                                                                                                                                                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTgg                      >  1:786421/1‑251 (MQ=255)                                                                                                                                                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTgg                      >  1:41641/1‑251 (MQ=255)                                                                                                                                                                                                     caGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGttt                   >  1:726217/1‑251 (MQ=255)                                                                                                                                                                                                       ggAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                 <  1:585140/203‑1 (MQ=255)                                                                                                                                                                                                       ggAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTAt                 >  1:32574/1‑251 (MQ=255)                                                                                                                                                                                                          gaATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg              >  1:650771/1‑251 (MQ=255)                                                                                                                                                                                                           aaTATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                         >  1:525723/1‑175 (MQ=255)                                                                                                                                                                                                           aaTATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg              <  1:498288/250‑1 (MQ=255)                                                                                                                                                                                                           aaTATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg              <  1:315666/250‑1 (MQ=255)                                                                                                                                                                                                            atatACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCAt                                                                                                                                                        >  1:672363/1‑111 (MQ=255)                                                                                                                                                                                                            atatACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTtat                                              <  1:666591/217‑1 (MQ=255)                                                                                                                                                                                                              ataCGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTc                                >  1:770648/1‑229 (MQ=255)                                                                                                                                                                                                                    tCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAAc    <  1:187706/251‑1 (MQ=255)                                                                                                                                                                                                                    tCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAAc    <  1:629203/251‑1 (MQ=255)                                                                                                                                                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGtt                                                                                                                                                                                            >  1:399308/1‑66 (MQ=255)                                                                                                                                                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                         >  1:565764/1‑165 (MQ=255)                                                                                                                                                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACa   <  1:800254/251‑1 (MQ=255)                                                                                                                                                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACa   <  1:112702/251‑1 (MQ=255)                                                                                                                                                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAccc                                                                >  1:289409/1‑189 (MQ=255)                                                                                                                                                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAt  >  1:268483/1‑251 (MQ=255)                                                                                                                                                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAt  <  1:764495/251‑1 (MQ=255)                                                                                                                                                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAt  >  1:472676/1‑251 (MQ=255)                                                                                                                                                                                                                       ttttAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACgg                                                                                <  1:374735/172‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             TTAATTATTTATATGTTCACCGTCGCTATTTACACCGTTGCCAATTCGATTACCTTGGAAGTGAAAAATGCCTCCGTTGCCATTGTGGATAACGATCACTCGGCGTTGTCTTATCGCCTGCAACAAAGCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCGCCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCCACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAT  >  1/1470020‑1470483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑