| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,466,308 | C→T | G33G (GGC→GGT) | unknown → | HlyD‑like membrane fusion protein YhiI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,466,308 | 0 | C | T | 98.7% | 214.6 / NA | 79 | G33G (GGC→GGT) | unknown | HlyD‑like membrane fusion protein YhiI |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (37/41); total (38/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CTCTCCTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGCATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACG > 1/1466066‑1466552 | ctctcCTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGc < 1:78333/251‑1 (MQ=255) ctcCTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCgg > 1:433318/1‑248 (MQ=255) ccTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGtt > 1:554078/1‑251 (MQ=255) ccTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGtt > 1:52699/1‑251 (MQ=255) ccTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGtt > 1:840852/1‑251 (MQ=255) aaTTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACgg < 1:488972/251‑1 (MQ=255) aTTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGa < 1:456870/251‑1 (MQ=255) ttACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACg < 1:268660/251‑1 (MQ=255) ttAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAAt > 1:79429/1‑251 (MQ=255) caacaaATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTaaaa < 1:297775/251‑1 (MQ=255) caacaaATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTaaaa < 1:164395/251‑1 (MQ=255) aacaaATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAAc < 1:52221/251‑1 (MQ=255) ttGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCa > 1:417837/1‑250 (MQ=255) tAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCAccc < 1:504311/250‑1 (MQ=255) tAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:419047/251‑1 (MQ=255) tAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:699337/251‑1 (MQ=255) tAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:601603/251‑1 (MQ=255) tAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:97502/251‑1 (MQ=255) aaaCGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCgtgt < 1:660598/251‑1 (MQ=255) aCGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTag < 1:275615/251‑1 (MQ=255) ttGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTagaag < 1:442929/251‑1 (MQ=255) ccTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAgaagaa < 1:279823/250‑1 (MQ=255) ggtTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTaaaa < 1:773461/199‑1 (MQ=255) gtTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTaaaa > 1:519596/1‑198 (MQ=255) gTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTa > 1:47124/1‑251 (MQ=255) ttttACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAAt < 1:302839/251‑1 (MQ=255) ttACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGa > 1:7945/1‑251 (MQ=255) aCCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAg < 1:774035/251‑1 (MQ=255) aCCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAg < 1:146489/251‑1 (MQ=255) aCCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAg < 1:226584/251‑1 (MQ=255) gATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTaaa > 1:75865/1‑251 (MQ=255) tctcTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATc < 1:853544/203‑1 (MQ=255) ctctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACg > 1:559666/1‑251 (MQ=255) ctctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACg > 1:103309/1‑251 (MQ=255) ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGaa > 1:131349/1‑169 (MQ=255) ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:63168/201‑1 (MQ=255) ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGaa > 1:311353/1‑232 (MQ=255) ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGc > 1:159844/1‑250 (MQ=255) ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCa > 1:729389/1‑251 (MQ=255) ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCa > 1:175796/1‑251 (MQ=255) cATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGCATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCAACCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGCAAAACGCAATCAAc > 1:669512/1‑250 (MQ=255) cGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTaa < 1:763034/226‑1 (MQ=255) cGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCa > 1:256207/1‑248 (MQ=255) gcgTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGGAATGAAGTAAAACGCAATCAACCACTGGCACGGCttt > 1:564856/1‑251 (MQ=255) tAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGc < 1:3087/234‑1 (MQ=255) ggATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:852809/168‑1 (MQ=255) gATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACg > 1:275494/1‑128 (MQ=255) gaTGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCgg < 1:151621/107‑1 (MQ=255) gaTGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTaa > 1:90373/1‑195 (MQ=255) aaGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGtt < 1:814626/251‑1 (MQ=255) atatTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc > 1:507866/1‑147 (MQ=255) atTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGca > 1:646707/1‑251 (MQ=255) tttCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTagaag > 1:266070/1‑158 (MQ=255) tttCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGcac < 1:82845/251‑1 (MQ=255) gTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCgg > 1:456026/1‑81 (MQ=255) gTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAg > 1:22785/1‑251 (MQ=255) tttCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAg < 1:43110/250‑1 (MQ=255) tCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCGCAGGCACAAAAGaaa < 1:32316/251‑1 (MQ=255) tCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGaaa < 1:237017/251‑1 (MQ=255) cATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATcc < 1:720115/129‑1 (MQ=255) ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATc > 1:133013/1‑126 (MQ=255) ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAgg > 1:365812/1‑237 (MQ=255) ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACgg < 1:620126/251‑1 (MQ=255) ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACgg > 1:610702/1‑251 (MQ=255) tatCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAAcc < 1:139499/180‑1 (MQ=255) ggTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTcc > 1:744872/1‑186 (MQ=255) gTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTccgc > 1:612061/1‑211 (MQ=255) ccATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGcc < 1:573440/251‑1 (MQ=255) ggCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCcaa > 1:365610/1‑250 (MQ=255) ggCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGAGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTttcttc > 1:797683/1‑153 (MQ=255) tgaactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCcaacaac < 1:567793/248‑1 (MQ=255) gaactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGt > 1:560991/1‑251 (MQ=255) aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTATAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc < 1:652558/251‑1 (MQ=255) aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc < 1:661309/251‑1 (MQ=255) aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc < 1:40427/251‑1 (MQ=255) aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTACTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc < 1:112555/251‑1 (MQ=255) gaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTccgc > 1:339351/1‑168 (MQ=255) gaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGcac > 1:767207/1‑179 (MQ=255) gaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACg > 1:463910/1‑251 (MQ=255) | CTCTCCTTAAATTTACCGAATTAAGCAACAAATTACGCCTTTTTGTAGGGTTAAGCAACAAAACGTTGCCTCTTGGTGGTTGAGTGGTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGCATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACG > 1/1466066‑1466552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |