Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,446,432	A→G	T1494A (ACA→GCA)	unknown →	Chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,446,432	0	A	G	100.0%	193.0 / NA	67	T1494A (ACA→GCA)	unknown	Chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/34);  total (33/34)

AAGCCGCCGTATTCGTGGCAAAGATATTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCACAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACTGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGTTGTCACTTTCTTGGTTTTTGTTAAAACCGGATTTCAAA  >  1/1446191‑1446674                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aaGCCGCCGTATTCGTGGCAAAGATATTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGagt                                                                                                                                                                                                                                           <  1:501435/251‑1 (MQ=255)     cgccgTATTCGTGGCAAAGATATTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGtt                                                                                                                                                                                                                                       <  1:101628/251‑1 (MQ=255)                        atatTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGagtag                                                                                                                                                                                                                                         <  1:627830/230‑1 (MQ=255)                        atatTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCgg                                                                                                                                                                                                                    >  1:739247/1‑251 (MQ=255)                        atatTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCgg                                                                                                                                                                                                                    >  1:218183/1‑251 (MQ=255)                        atatTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCgg                                                                                                                                                                                                                    >  1:160400/1‑251 (MQ=255)                             gtgccgtgccGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGAGCGGTTaaa                                                                                                                                                                                                               >  1:675457/1‑251 (MQ=255)                                    gccgCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATaaa                                                                                                                                                                                                        >  1:170528/1‑251 (MQ=255)                                     ccgCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAg                                                                                                                                                                                                       <  1:679641/251‑1 (MQ=255)                                      cgCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAgt                                                                                                                                                                                                      <  1:260201/251‑1 (MQ=255)                                                    ggACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGagtag                                                                                                                                                                                                                                         <  1:843618/202‑1 (MQ=255)                                                            cggcgCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTAtt                                                                                                                                                                                 <  1:243823/250‑1 (MQ=255)                                                            cggcgCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTAt                                                                                                                                                                                  <  1:69318/249‑1 (MQ=255)                                                                 cgTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTa                                                                                                                                                                           >  1:871638/1‑251 (MQ=255)                                                                               aTCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATaaaaaa                                                                                                                                                             >  1:745798/1‑251 (MQ=255)                                                                                            ttATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCAt                                                                                                                                                <  1:659203/251‑1 (MQ=255)                                                                                             tATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCAtt                                                                                                                                               >  1:8671/1‑251 (MQ=255)                                                                                              aTTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTa                                                                                                                                              <  1:381482/251‑1 (MQ=255)                                                                                              aTTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTa                                                                                                                                              <  1:688888/251‑1 (MQ=255)                                                                                                tCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATc                                                                                                                                            <  1:168567/251‑1 (MQ=255)                                                                                                  gAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCGCAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCg                                                                                                                                           >  1:80887/1‑250 (MQ=255)                                                                                                       gtgCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTc                                                                                                                                      >  1:415923/1‑250 (MQ=255)                                                                                                         gCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATaaaa                                                                                                                                                               >  1:97756/1‑223 (MQ=255)                                                                                                            aaCGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAAt                                                                                                                                                                                                           <  1:620852/176‑1 (MQ=255)                                                                                                            aaCGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgt                                                                                                                                <  1:652973/251‑1 (MQ=255)                                                                                                                gCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtagcg                                                                                                                            >  1:795216/1‑251 (MQ=255)                                                                                                                 cTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTCCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtagcgt                                                                                                                           <  1:454742/251‑1 (MQ=255)                                                                                                                  tAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtagcgta                                                                                                                          >  1:401901/1‑251 (MQ=255)                                                                                                                    gATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGAc                                                                                                                                                                                            >  1:231411/1‑183 (MQ=255)                                                                                                                    gATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTcc                                                                                                                                     <  1:296070/238‑1 (MQ=255)                                                                                                                    gATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtagcgtag                                                                                                                         >  1:5156/1‑250 (MQ=255)                                                                                                                     atatGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGtagcgtagcg                                                                                                                       >  1:767528/1‑251 (MQ=255)                                                                                                                     atatGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGtagcgtagcg                                                                                                                       >  1:599488/1‑251 (MQ=255)                                                                                                                      tatGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCgg                                                                                                                      <  1:410074/251‑1 (MQ=255)                                                                                                                       atGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGt                                                                                                                     >  1:395178/1‑251 (MQ=255)                                                                                                                              tGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTa                                                                                                              <  1:144318/251‑1 (MQ=255)                                                                                                                               gCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTa                                                                                                              >  1:381500/1‑250 (MQ=255)                                                                                                                               gCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTa                                                                                                              >  1:651633/1‑250 (MQ=255)                                                                                                                                               gAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGagt                                                                                                                                                                                                                                           >  1:977/1‑109 (MQ=255)                                                                                                                                                     atCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCg                                                                                       <  1:494542/251‑1 (MQ=255)                                                                                                                                                          cccGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAGCAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGGCCGCAAGCGTaa                                                                                    <  1:40455/249‑1 (MQ=255)                                                                                                                                                           ccGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAatat                                                                                 <  1:174307/251‑1 (MQ=255)                                                                                                                                                           ccGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAatat                                                                                 <  1:109371/251‑1 (MQ=255)                                                                                                                                                           ccGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAatat                                                                                 <  1:340806/251‑1 (MQ=255)                                                                                                                                                             ggAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCg                                                                               >  1:7986/1‑251 (MQ=255)                                                                                                                                                              gAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtag                                                                                                                              >  1:304706/1‑203 (MQ=255)                                                                                                                                                               aaaaaGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGt                                                                              <  1:33211/250‑1 (MQ=255)                                                                                                                                                                       acaACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAgg                                                                                                                                                                      <  1:440980/154‑1 (MQ=255)                                                                                                                                                                           ccTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAatat                                                                                 >  1:901548/1‑235 (MQ=255)                                                                                                                                                                           ccTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCt                                                                 <  1:522645/251‑1 (MQ=255)                                                                                                                                                                            cTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTa                                                                >  1:483231/1‑251 (MQ=255)                                                                                                                                                                              aCAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTAtt                                                              >  1:607689/1‑251 (MQ=255)                                                                                                                                                                                     ggTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGaaa                                                                                                     <  1:889035/205‑1 (MQ=255)                                                                                                                                                                                       tGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAAc                                                     <  1:419846/251‑1 (MQ=255)                                                                                                                                                                                            aaaaTTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATAc                                                <  1:100275/251‑1 (MQ=255)                                                                                                                                                                                            aaaaTTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATAc                                                <  1:475892/251‑1 (MQ=255)                                                                                                                                                                                                    cGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGt                                        <  1:162867/251‑1 (MQ=255)                                                                                                                                                                                                                   cGTTCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGTTGTCACTTTCTTGGt                         >  1:36029/1‑251 (MQ=255)                                                                                                                                                                                                                     ttCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtag                                                                                                                              <  1:171324/148‑1 (MQ=255)                                                                                                                                                                                                                     ttCATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGTTGTCACTTTCTTGGttt                       >  1:485150/1‑251 (MQ=255)                                                                                                                                                                                                                       cATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTgtag                                                                                                                              <  1:784458/146‑1 (MQ=255)                                                                                                                                                                                                                       cATGTAGTCGGCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATAc                                                <  1:796881/224‑1 (MQ=255)                                                                                                                                                                                                                                ggCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCAt                                                          >  1:710239/1‑205 (MQ=255)                                                                                                                                                                                                                                 gCTTACGCGGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGTTGTCACTTTCTTGGTTTTTGTTAAAACCg           >  1:635898/1‑251 (MQ=255)                                                                                                                                                                                                                                      cgcgGATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGt                                                                                                   >  1:667638/1‑158 (MQ=255)                                                                                                                                                                                                                                          gATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGaa                                                                                                      >  1:793881/1‑151 (MQ=255)                                                                                                                                                                                                                                          gATTCGGCGCAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACCGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGTTGTCACTTTCTTGGTTTTTGTTAAAACCGGATTTCaaa  >  1:357305/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   AAGCCGCCGTATTCGTGGCAAAGATATTGTGCCGTGCCGCTTATTGTTCTTGGACGAAGCGGCGCGTTTGGATGCCAAATCTATTTCCACTTTATTCGAGCTGTGCGAACGGCTAGATATGCAATTGCTCATCGCCGCCCCGGAAAATATCAGCCCGGAAAAAGGCACAACCTACAAACTGGTGCGGAAAATTTCCGGTAATCACGAACACGTTCATGTAGTCGGCTTACGCGGATTCGGCACAACAGAGTAGTTTGTAAAAGAAAAAGTGCGGTTAAAAAATAAAGTGTTTTTTGACTGCACTTTATTTCATTAAAAGGAATAAAAAATGCACAATCTCATTATCGCCGTCCTCTGTAGCGTAGCGGTTTCCGTACTATTGAAAGTTGCCCGCAAGCGTAATATCGTTATTCAACAAGCTATTGCATTTAACTATACTGTTGCGTTGTCACTTTCTTGGTTTTTGTTAAAACCGGATTTCAAA  >  1/1446191‑1446674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑