Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,430,622	G→A	V19M (GTG→ATG)	unknown →	Glycogen phosphorylase (EC 2.4.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,430,622	0	G	A	100.0%	91.1 / NA	34	V19M (GTG→ATG)	unknown	Glycogen phosphorylase (EC 2.4.1.1)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/18);  total (16/18)

GTAATGAAATAGTTAATCTTTCGTTCAAGTAAAAATACGGCTTGAACGGAAGATTATTATTGTTTTTTTATCTCGAATGATTAAAATTTCACCTTTTTTTTGATTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATGTGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTCTAACGCCATGATTGC  >  1/1430442‑1430854                                                                                                                                                                                     |                                                                                                                                                                                                                                         gTAATGAAATAGTTAATCTTTCGTTCAAGTAAAAATACGGCTTGAACGGAAGATTATTATTGTTTTTTTATCTCGAATGATTAAAATTTCACCTTTTTTTTGATTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAag                                                                                                                                                                           <  1:30756/244‑1 (MQ=255) gTAATGAAATAGTTAATCTTTCGTTCAAGTAAAAATACGGCTTGAACGGAAGATTATTATTGTTTTTTTATCTCGAATGATTAAAATTTCACCTTTTTTTTGATTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAg                                                                                                                                                                    >  1:430481/1‑251 (MQ=255)                                                                 tttttATCTCGAATGATTAAAATTTCACCTTTTTTTTGATTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGAt                                                                                                     >  1:527694/1‑250 (MQ=255)                                                                 tttttATCTCGAATGATTAAAATTTCACCTTTTTTTTGATTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGAt                                                                                                     >  1:70081/1‑250 (MQ=255)                                                                                                       aTTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAag                                                                                                                                                                           >  1:717596/1‑142 (MQ=255)                                                                                                                 gAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGc                                                                                                                                   <  1:319200/172‑1 (MQ=255)                                                                                                                   ggACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTc                                                                                                                                                                  <  1:188223/139‑1 (MQ=255)                                                                                                                   ggACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAg                                                                                                            <  1:113283/193‑1 (MQ=255)                                                                                                                      cTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGc                                                                                                                                   >  1:821467/1‑167 (MQ=255)                                                                                                                      cTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGc                                                                                                                                   >  1:58027/1‑167 (MQ=255)                                                                                                                      cTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTcgcg                                                            <  1:680971/238‑1 (MQ=255)                                                                                                                        aTTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGc                                                                                                                                   <  1:325913/165‑1 (MQ=255)                                                                                                                        aTTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATc                                               >  1:669582/1‑249 (MQ=255)                                                                                                                        aTTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCa                                             >  1:357487/1‑251 (MQ=255)                                                                                                                        aTTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCa                                             >  1:125171/1‑251 (MQ=255)                                                                                                                        aTTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCACCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGACTAAAGAGGCAAGCCAGCGCGATTGGTTAAAAGCGACCTTATATGCAGTGCGTGATTTTGTGAAGGAAGGGTGGATAAACACAGCCCGCCGAGCCAGAACGGAAGGGTCACGTCGCGTTTATTATTTATCCa                                             >  1:472159/1‑251 (MQ=255)                                                                                                                           ttATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAAcg                                                                                                                                                >  1:187389/1‑149 (MQ=255)                                                                                                                                     aTGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAATTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGAt                                <  1:438661/251‑1 (MQ=255)                                                                                                                                     aTGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAg                                                                                                                                 <  1:714525/154‑1 (MQ=255)                                                                                                                                     aTGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGAt                                <  1:204757/251‑1 (MQ=255)                                                                                                                                     aTGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGA‑CTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGt                                                                <  1:554912/218‑1 (MQ=255)                                                                                                                                       ggATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCg                              >  1:668959/1‑251 (MQ=255)                                                                                                                                             cTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTgaag                                                                                                                                                                                                                            <  1:483239/55‑1 (MQ=255)                                                                                                                                             cTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCa                        >  1:64851/1‑251 (MQ=255)                                                                                                                                                cGATTCTCCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATCGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACtt                     <  1:415167/251‑1 (MQ=255)                                                                                                                                                    tctcCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTc                 <  1:807821/251‑1 (MQ=255)                                                                                                                                                     ctcCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCtt                                                                                                                                                                                              <  1:315983/77‑1 (MQ=255)                                                                                                                                                     ctcCATTCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTCAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAAc                                                                             >  1:409659/1‑190 (MQ=255)                                                                                                                                                          ttCTTGTACGATCATTCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAAc                                                                             <  1:431765/185‑1 (MQ=255)                                                                                                                                                          ttCTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTCTAACGc           <  1:681722/251‑1 (MQ=255)                                                                                                                                                          tcggggatgtgtataagggacagcAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTCTAACGc           <  1:114841/228‑1 (MQ=255)                                                                                                                                                            cTTGTACGATCATCCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTCTAACGCCa         >  1:781956/1‑251 (MQ=255)                                                                                                                                                                    atcatcCCGAAATCAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTCTAACGCCATGATTGc  >  1:569628/1‑250 (MQ=255)                                                                                                                                                                                 cAATATGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAGCGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTg                                  <  1:630779/205‑1 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                                         GTAATGAAATAGTTAATCTTTCGTTCAAGTAAAAATACGGCTTGAACGGAAGATTATTATTGTTTTTTTATCTCGAATGATTAAAATTTCACCTTTTTTTTGATTGTTAACTGAGGACTATTTTATATGATAATGGATAACTTCGATTCTCCATTCTTGTACGATCATCCCGAAATCAATGTGGCATCATTGAAGAAGGCGATCGTCTATAAACTGATTTTTCTTATCGGGCGCTCGCCTAAAGAGGCAAGTCAGCGCGATTGGTTAAACGCGACCTTATATGCAGTGCGTGATTTTGTGACGGAAGGTTGGATCACCACAGCCCGCCGAGCCAGAACGGAAGGTTCACGTCGCGTTTATTATTTATCCATGGAGTTTTTGATCGGTCGCACTTTGTCTAACGCCATGATTGC  >  1/1430442‑1430854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑