Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,428,381 C→G F296L (TTC→TTG)  unknown → Glucose‑1‑phosphate adenylyltransferase (EC 2.7.7.27)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,428,3810CG100.0% 214.2 / NA 70F296L (TTC→TTG) unknownGlucose‑1‑phosphate adenylyltransferase (EC 2.7.7.27)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (31/39);  total (31/39)

GATTATCTCTATGAAATGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTCGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAAC  >  1/1428151‑1428626
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
gATTATCTCTATGAAATGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAAc                                                                                                                                                                                                                                      >  1:278343/1‑248 (MQ=255)
             aaaTGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGt                                                                                                                                                                                                                      <  1:774461/251‑1 (MQ=255)
                    gAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCgg                                                                                                                                                                                                               >  1:593244/1‑251 (MQ=255)
                      aTCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTg                                                                                                                                                                                                             >  1:34421/1‑251 (MQ=255)
                          ggAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAg                                                                                                                                                                                                         >  1:761009/1‑251 (MQ=255)
                                    acGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATc                                                                                                                                                                                               <  1:373769/251‑1 (MQ=255)
                                      gCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATcc                                                                                                                                                                                                  <  1:417352/246‑1 (MQ=255)
                                         ttGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAAt                                                                                                                                                                                          <  1:568340/251‑1 (MQ=255)
                                                tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATcc                                                                                                                                                                                                                 <  1:881705/221‑1 (MQ=255)
                                                tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATcc                                                                                                                                                                                                  <  1:820426/236‑1 (MQ=255)
                                                tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTta                                                                                                                                                                                   >  1:271088/1‑251 (MQ=255)
                                                tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTta                                                                                                                                                                                   >  1:269452/1‑251 (MQ=255)
                                                 ctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTtat                                                                                                                                                                                  >  1:691597/1‑251 (MQ=255)
                                                       aCTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATaaaaa                                                                                                                                                                             >  1:862850/1‑250 (MQ=255)
                                                            ggTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCaa                                                                                                                                                                       >  1:438632/1‑251 (MQ=255)
                                                               aaaGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAatat                                                                                                                                                                    <  1:455437/251‑1 (MQ=255)
                                                                    cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                               <  1:844517/251‑1 (MQ=255)
                                                                    cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                               <  1:700332/251‑1 (MQ=255)
                                                                    cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATAGCAAAc                                                                                                                                                               <  1:268593/251‑1 (MQ=255)
                                                                       aTTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCgg                                                                                                                                                            <  1:747721/251‑1 (MQ=255)
                                                                            gcatttcatagagataatcggcgTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGcc                                                                                                                                                                                                       <  1:801907/183‑1 (MQ=255)
                                                                                  gTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCttt                                                                                                                                                                                     <  1:872258/215‑1 (MQ=255)
                                                                                  gTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAAct                                                                                                                                                   >  1:84025/1‑249 (MQ=255)
                                                                                           aagGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                         <  1:877786/250‑1 (MQ=255)
                                                                                                  gTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCttt                                                                                                                                                                                     <  1:33752/199‑1 (MQ=255)
                                                                                                   ttATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                               >  1:844309/1‑220 (MQ=255)
                                                                                                   ttATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTa                                                                                                                                             <  1:767300/238‑1 (MQ=255)
                                                                                                     ataCGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCg                                                                                                                              <  1:271412/251‑1 (MQ=255)
                                                                                                       aCGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTa                                                                                                                                                                                                                                               <  1:181631/136‑1 (MQ=255)
                                                                                                              cccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCg                                                                                                                     >  1:745837/1‑251 (MQ=255)
                                                                                                              cccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCg                                                                                                                     >  1:446270/1‑251 (MQ=255)
                                                                                                               ccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                         <  1:9265/230‑1 (MQ=255)
                                                                                                                          cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGatat                                                                                                                                                                                                                                                  <  1:434690/114‑1 (MQ=255)
                                                                                                                          cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCatcaatc                                                                                                          <  1:556012/250‑1 (MQ=255)
                                                                                                                          cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCatcaa                                                                                                            <  1:68321/248‑1 (MQ=255)
                                                                                                                               gCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGt                                                                                                                                        >  1:106719/1‑215 (MQ=255)
                                                                                                                               gCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTAt                                                                                                    >  1:469734/1‑251 (MQ=255)
                                                                                                                                 aTGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                  <  1:649289/251‑1 (MQ=255)
                                                                                                                                    ggACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCg                                                                                                <  1:636167/250‑1 (MQ=255)
                                                                                                                                      aCGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                  >  1:474069/1‑246 (MQ=255)
                                                                                                                                        gTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGtgttg                                                                                           >  1:643327/1‑251 (MQ=255)
                                                                                                                                             aCCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                         <  1:833560/200‑1 (MQ=255)
                                                                                                                                                  aGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCa                                                                                 >  1:851673/1‑251 (MQ=255)
                                                                                                                                                         atTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGat                                                                                                                                                                                                                                                    <  1:660160/81‑1 (MQ=255)
                                                                                                                                                         atTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCgtgt                                                                                             >  1:91418/1‑232 (MQ=255)
                                                                                                                                                           ttACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTa                                                                        >  1:101715/1‑251 (MQ=255)
                                                                                                                                                                ggCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCat                                                                                                               <  1:175970/207‑1 (MQ=255)
                                                                                                                                                                       cGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTa                                                                                                                                                                                                                                               >  1:820094/1‑72 (MQ=255)
                                                                                                                                                                        gTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCa                                                           >  1:554853/1‑251 (MQ=255)
                                                                                                                                                                         tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACATCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAAt                                                                          <  1:803490/235‑1 (MQ=255)
                                                                                                                                                                         tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCg                                                                                   <  1:572413/226‑1 (MQ=255)
                                                                                                                                                                         tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                          >  1:543034/1‑251 (MQ=255)
                                                                                                                                                                         tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                          >  1:273311/1‑251 (MQ=255)
                                                                                                                                                                         tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                          >  1:583327/1‑251 (MQ=255)
                                                                                                                                                                           ggTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCGAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGAc                                                                                     <  1:84044/222‑1 (MQ=255)
                                                                                                                                                                           ggTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGATGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAAt                                                        >  1:456311/1‑251 (MQ=255)
                                                                                                                                                                              aCATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAgg                                                                 <  1:395093/239‑1 (MQ=255)
                                                                                                                                                                                   ggACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGAt                                                                                                                   <  1:374450/184‑1 (MQ=255)
                                                                                                                                                                                    gACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCAtt                                                >  1:351639/1‑250 (MQ=255)
                                                                                                                                                                                        gCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGt                                                                                                                                        >  1:448199/1‑158 (MQ=255)
                                                                                                                                                                                         cTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCg                                             <  1:893525/248‑1 (MQ=255)
                                                                                                                                                                                           tCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCgtggtg                                        <  1:682402/251‑1 (MQ=255)
                                                                                                                                                                                                 aaTCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCg                                  <  1:409862/251‑1 (MQ=255)
                                                                                                                                                                                                  aTCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCg                                  >  1:137141/1‑250 (MQ=255)
                                                                                                                                                                                                       atatCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAAt                                                        >  1:88344/1‑223 (MQ=255)
                                                                                                                                                                                                          tCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCTATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGaa                         <  1:432007/251‑1 (MQ=255)
                                                                                                                                                                                                                    ttCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                  <  1:339580/168‑1 (MQ=255)
                                                                                                                                                                                                                    ttCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAAt                                                                          <  1:389419/192‑1 (MQ=255)
                                                                                                                                                                                                                              ccGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGtt     >  1:546363/1‑251 (MQ=255)
                                                                                                                                                                                                                                 cAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAac  <  1:135524/251‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
GATTATCTCTATGAAATGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTCGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAAC  >  1/1428151‑1428626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑