Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,419,242	A→G	F324L (TTT→CTT)	unknown ←	Macrolide‑specific efflux protein MacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,419,242	0	A	G	100.0%	202.7 / NA	67	F324L (TTT→CTT)	unknown	Macrolide‑specific efflux protein MacA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/39);  total (28/39)

ATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAACAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAATTTTGGCGTGATAAACGGTTTTATTGTCGGATAAAAT  >  1/1418997‑1419485                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aTTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAgcaaca                                                                                                                                                                                                                                                >  1:742390/1‑251 (MQ=255) aTTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAgcaaca                                                                                                                                                                                                                                                >  1:630520/1‑251 (MQ=255)  ttATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCGCCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAacac                                                                                                                                                                                                                                               <  1:320212/251‑1 (MQ=255)  ttATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAacac                                                                                                                                                                                                                                               <  1:531542/251‑1 (MQ=255)  ttATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAacac                                                                                                                                                                                                                                               <  1:643224/251‑1 (MQ=255)    atatTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACAt                                                                                                                                                                                                                                             <  1:211856/251‑1 (MQ=255)           tcgtcgAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGcac                                                                                                                                                                                                                                      <  1:745466/251‑1 (MQ=255)             gtcgAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACAt                                                                                                                                                                                                                                    >  1:582959/1‑251 (MQ=255)             gtcgAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACAt                                                                                                                                                                                                                                    >  1:406482/1‑251 (MQ=255)                           cACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATg                                                                                                                                                                                                                      <  1:337767/251‑1 (MQ=255)                             cTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATg                                                                                                                                                                                                                      >  1:706606/1‑249 (MQ=255)                                   aagaTAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTc                                                                                                                                                                                                              <  1:94994/251‑1 (MQ=255)                                     gaTAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCg                                                                                                                                                                                                            >  1:359181/1‑251 (MQ=255)                                               gCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATAc                                                                                                                                                                                                   >  1:399120/1‑250 (MQ=255)                                                cATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATAcc                                                                                                                                                                                                  <  1:276925/250‑1 (MQ=255)                                                cATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCa                                                                                                                                                                                                 >  1:725188/1‑251 (MQ=255)                                                cATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCa                                                                                                                                                                                                 >  1:717689/1‑251 (MQ=255)                                                  tCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAAt                                                                                                                                                                                               <  1:199968/251‑1 (MQ=255)                                                      gATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATAc                                                                                                                                                                                             <  1:564767/249‑1 (MQ=255)                                                       aTCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTTCTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGc                                                                                                                                                                                           <  1:527193/250‑1 (MQ=255)                                                       aTCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGc                                                                                                                                                                                           <  1:163530/250‑1 (MQ=255)                                                                ggTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGCTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGcac                                                                                                                                                                                                                                      >  1:571494/1‑198 (MQ=255)                                                                      aCCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGAt                                                                                                                                                                           >  1:199151/1‑251 (MQ=255)                                                                                   tgtgAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAAt                                                                                                                                                              >  1:97970/1‑251 (MQ=255)                                                                                          tCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCg                                                                                                                                                       <  1:573811/251‑1 (MQ=255)                                                                                           cACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCCCATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAAc                                                                                                                                                                                      >  1:430403/1‑219 (MQ=255)                                                                                             ctactTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGtaa                                                                                                                                                    >  1:35574/1‑251 (MQ=255)                                                                                               actTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATAcc                                                                                                                                                                                                  <  1:23044/203‑1 (MQ=255)                                                                                                ctTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAAt                                                                                                                                                                                               >  1:53913/1‑205 (MQ=255)                                                                                                     tCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:63963/251‑1 (MQ=255)                                                                                                     tCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:200260/251‑1 (MQ=255)                                                                                                     tCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:468086/251‑1 (MQ=255)                                                                                                     tCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:872665/251‑1 (MQ=255)                                                                                                     tCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:14396/251‑1 (MQ=255)                                                                                                     tCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTAGTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:441752/251‑1 (MQ=255)                                                                                                        ccTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTg                                                                                                                                          <  1:532411/250‑1 (MQ=255)                                                                                                          ttCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTgcgc                                                                                                                                       <  1:805854/251‑1 (MQ=255)                                                                                                            ccGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGtaat                                                                                                                                                   <  1:286506/237‑1 (MQ=255)                                                                                                                       gATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTc                                                                                                                                                                                                              >  1:99564/1‑167 (MQ=255)                                                                                                                       gATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAAc                                                                                                                           <  1:105106/250‑1 (MQ=255)                                                                                                                                         aaaTCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAAcc                                                                                                        <  1:307626/251‑1 (MQ=255)                                                                                                                                                    cACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAAc                                                                                             <  1:729879/251‑1 (MQ=255)                                                                                                                                                                   ttcCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAAt                                                                              <  1:396446/251‑1 (MQ=255)                                                                                                                                                                         ttCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCaa                                                                                                                                                                                                                              >  1:124529/1‑101 (MQ=255)                                                                                                                                                                         ttCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCggg                                                                                                                                                                             <  1:18546/150‑1 (MQ=255)                                                                                                                                                                           cTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAatat                                                                                                                                              <  1:375179/179‑1 (MQ=255)                                                                                                                                                                           cTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:565298/181‑1 (MQ=255)                                                                                                                                                                              gtgtTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCt                                                                                   <  1:837558/235‑1 (MQ=255)                                                                                                                                                                                gttgtATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGt                                                                                                                                                                                                        >  1:64562/1‑116 (MQ=255)                                                                                                                                                                                   gtATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATCTACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAAc                                                                                             >  1:455326/1‑220 (MQ=255)                                                                                                                                                                                   gtATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATggtggt                                                              >  1:735810/1‑251 (MQ=255)                                                                                                                                                                                           gTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            <  1:86654/165‑1 (MQ=255)                                                                                                                                                                                             ttAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGc                                                                                                                                                                                           >  1:572372/1‑116 (MQ=255)                                                                                                                                                                                             ttAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCggg                                                    <  1:745730/251‑1 (MQ=255)                                                                                                                                                                                                gTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCt                                                                                   <  1:726523/217‑1 (MQ=255)                                                                                                                                                                                                       gACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAAt                                          >  1:441684/1‑251 (MQ=255)                                                                                                                                                                                                         cTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTg                                                                                                                                          <  1:85672/153‑1 (MQ=255)                                                                                                                                                                                                            cATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATAcc                                                                                                                                                                                                  >  1:658872/1‑94 (MQ=255)                                                                                                                                                                                                             atatTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATAc                                                                                                                                                                                             <  1:777426/98‑1 (MQ=255)                                                                                                                                                                                                             atatTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCg                                                                                                                                                       >  1:858214/1‑136 (MQ=255)                                                                                                                                                                                                              tatTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAAtttt                                   >  1:827350/1‑251 (MQ=255)                                                                                                                                                                                                              tatTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAAtttt                                   >  1:459481/1‑251 (MQ=255)                                                                                                                                                                                                                           gctTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCa                                                                                                                                                                                                 <  1:178577/80‑1 (MQ=255)                                                                                                                                                                                                                           gctTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATAc                                                                                                                                            >  1:639300/1‑133 (MQ=255)                                                                                                                                                                                                                           gctTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAAtttt                                   >  1:523141/1‑238 (MQ=255)                                                                                                                                                                                                                           gctTTTGCACTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAATTTTGGCGTGATAAACg                      >  1:241535/1‑251 (MQ=255)                                                                                                                                                                                                                                    cTGCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAATTTTGGCGTGAGAAACGGTTTTATTg             <  1:421608/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCCAAATTCGGAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTg                                                                                                                                          >  1:313105/1‑124 (MQ=255)                                                                                                                                                                                                                                                gAATAAGCAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAATTTTGGCGTGATAAACGGTTTTATTGTCGGATAAAAt  <  1:584820/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGATCGCCGAAGGTTTCACCGGCAGCAACTTGTGAAATCACTACTTTTTCGCCTTCCGTTAAACCGGATTTGACTTCCGTTTGGAAATCATTTTGCACGCCGATTTCGATTTCCCGTTCTTGTGTTGTATTGCCGTTTAGTACATTGACTACATATTTGTCTTGCTGCTTTTGCACTGCCAAATTCGGAATAAACAACACATTTTGCACATCGGCAATTTTAATGTTATTTTCCGTGGTCATACCAATACGCAAAACATGTTCGGGATTTTCAACGATAATGTTGGCGTAATAATATACTGCGCTGCTGCTTGAACCGGAGGAGGAACTGGAACCTGAGCTTGAACTGTTTGAGCTTGAATTGTCGCTGATGGTGGTTGTTGCCGGGTCTACGGAATCAATTTTGGCGTGATAAACGGTTTTATTGTCGGATAAAAT  >  1/1418997‑1419485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑