Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,397,171	C→A	I198I (ATC→ATA)	unknown →	PTS system, galactitol‑specific IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,397,171	0	C	A	100.0%	226.3 / NA	79	I198I (ATC→ATA)	unknown	PTS system, galactitol‑specific IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/36);  total (43/36)

TTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATCGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTAACACCCATTGCCAAGCAAGCACGTA  >  1/1396945‑1397415                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     tttGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATaaa                                                                                                                                                                                                                              >  1:80837/1‑251 (MQ=255)  ttGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAg                                                                                                                                                                                                                             <  1:894659/251‑1 (MQ=255)   tGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGt                                                                                                                                                                                                                            <  1:234849/251‑1 (MQ=255)       atatCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAAt                                                                                                                                                                                                                                 <  1:752269/242‑1 (MQ=255)         atCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCAttt                                                                                                                                                                                                                      >  1:290861/1‑251 (MQ=255)         atCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCAttt                                                                                                                                                                                                                      >  1:840941/1‑251 (MQ=255)               cATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTccgc                                                                                                                                                                                                                <  1:670931/251‑1 (MQ=255)                      ccTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCgatgat                                                                                                                                                                                                         >  1:187216/1‑251 (MQ=255)                                  ccATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATaa                                                                                                                                                                                                                               >  1:45330/1‑217 (MQ=255)                                       gtgCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATaa                                                                                                                                                                                                                               <  1:697700/212‑1 (MQ=255)                                       gtgCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCgg                                                                                                                                                                                        >  1:150854/1‑251 (MQ=255)                                          cATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGAc                                                                                                                                                                                      <  1:343926/250‑1 (MQ=255)                                              tCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGtt                                                                                                                                                                                  >  1:87272/1‑250 (MQ=255)                                                       gTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAg                                                                                                                                                                                                                             <  1:169878/198‑1 (MQ=255)                                                                ggCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTtctc                                                                                                                                                                                                                   <  1:895010/199‑1 (MQ=255)                                                                    gggTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTc                                                                                                                                                                     >  1:724486/1‑241 (MQ=255)                                                                      gTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGttt                                                                                                                                                          <  1:580580/250‑1 (MQ=255)                                                                          tctcGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCa                                                                                                                                                     >  1:184266/1‑251 (MQ=255)                                                                          tctcGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCa                                                                                                                                                     <  1:462474/251‑1 (MQ=255)                                                                                ggTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATgggg                                                                                                                                                <  1:188759/250‑1 (MQ=255)                                                                                 gTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGat                                                                                                                                              >  1:890825/1‑251 (MQ=255)                                                                                      gATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGattattt                                                                                                                                         >  1:811884/1‑250 (MQ=255)                                                                                      gATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTCGTCATGGGGAttatta                                                                                                                                         >  1:383589/1‑251 (MQ=255)                                                                                       aTTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATtattat                                                                                                                                        <  1:887999/251‑1 (MQ=255)                                                                                          cACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTg                                                                                                                                                                                                                           >  1:613340/1‑165 (MQ=255)                                                                                                gCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTc                                                                                                                                                      <  1:667647/228‑1 (MQ=255)                                                                                                gCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTAtttt                                                                                                                               >  1:665361/1‑251 (MQ=255)                                                                                                gCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTAtttt                                                                                                                               >  1:706620/1‑251 (MQ=255)                                                                                                   tttgtttACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTgg                                                                                                                             >  1:128574/1‑250 (MQ=255)                                                                                                   tttgtttACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTgg                                                                                                                             <  1:619528/250‑1 (MQ=255)                                                                                                             aaCTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCg                                                                                                                  >  1:48470/1‑251 (MQ=255)                                                                                                                  ggAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATtattat                                                                                                                                        >  1:167116/1‑224 (MQ=255)                                                                                                                  ggAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTaaa                                                                                                             >  1:581695/1‑251 (MQ=255)                                                                                                                          gTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTgg                                                                                                                             >  1:624934/1‑227 (MQ=255)                                                                                                                          gTTTAGTCGCGATACGAAAAATTTCTTGGAACTGGACGGCATTGCCATTCCGCACGGCGCATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTgcggcgcccggccagccgatatcc                                                                                                                                                           <  1:425115/197‑19 (MQ=255)                                                                                                                                             aaTTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAAc                                                                                  <  1:575278/251‑1 (MQ=255)                                                                                                                                              aTTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCAGCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAAcc                                                                                 <  1:452311/251‑1 (MQ=255)                                                                                                                                                 tCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGc                                                                                                                            >  1:299145/1‑205 (MQ=255)                                                                                                                                                      gAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGt                                                                         >  1:310675/1‑251 (MQ=255)                                                                                                                                                                gCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCa                                                                                                                                                     <  1:350584/165‑1 (MQ=255)                                                                                                                                                                gCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTa                                                               >  1:577884/1‑251 (MQ=255)                                                                                                                                                                gCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTa                                                               >  1:103862/1‑251 (MQ=255)                                                                                                                                                                   ttGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGATCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAAtt                                                            >  1:790621/1‑250 (MQ=255)                                                                                                                                                                   ttGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATg                                                            >  1:897493/1‑251 (MQ=255)                                                                                                                                                                    tGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGc                                                           <  1:109190/251‑1 (MQ=255)                                                                                                                                                                     gCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGc                                                           >  1:542523/1‑250 (MQ=255)                                                                                                                                                                        aTTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGc                                                           >  1:677968/1‑247 (MQ=255)                                                                                                                                                                           ccGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTaa                                                                                                              >  1:317174/1‑193 (MQ=255)                                                                                                                                                                               aCGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCAtt                                                <  1:385418/251‑1 (MQ=255)                                                                                                                                                                                  gCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTaaa                                             >  1:43594/1‑251 (MQ=255)                                                                                                                                                                                   cacaTCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAAc                                            <  1:626698/251‑1 (MQ=255)                                                                                                                                                                                   cacaTCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAAc                                            <  1:789468/251‑1 (MQ=255)                                                                                                                                                                                       tCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAAt                                        >  1:218185/1‑251 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGAc                                                                                                                                                                                      >  1:592112/1‑102 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCg                                                                                                                  >  1:677482/1‑170 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGAtg                                                                     <  1:5433/215‑1 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGAtg                                                                     <  1:836354/215‑1 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGc                                                           >  1:224389/1‑225 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGAc                                 <  1:229006/251‑1 (MQ=255)                                                                                                                                                                                              atTTGGGGCCGATCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGAc                                 <  1:636796/251‑1 (MQ=255)                                                                                                                                                                                                          tCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGc                                                           >  1:255549/1‑213 (MQ=255)                                                                                                                                                                                                          tCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAAc                                            <  1:818536/228‑1 (MQ=255)                                                                                                                                                                                                          tCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaaga                     >  1:751927‑M1/1‑244 (MQ=255)                                                                                                                                                                                                          tCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaag                      <  1:364505‑M1/250‑7 (MQ=255)                                                                                                                                                                                                          tCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaag                      >  1:722336‑M1/1‑244 (MQ=255)                                                                                                                                                                                                          tCGCAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaa                       >  1:748997‑M1/1‑244 (MQ=255)                                                                                                                                                                                                             cAGTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAg                                                                                                >  1:471083/1‑173 (MQ=255)                                                                                                                                                                                                               gTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAAt                                                                                                                                                                                                                                 >  1:368563/1‑42 (MQ=255)                                                                                                                                                                                                               gTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGc                                                           <  1:113893/208‑1 (MQ=255)                                                                                                                                                                                                               gTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaagaatgt                 >  1:619695‑M1/1‑239 (MQ=255)                                                                                                                                                                                                               gTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCGTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGAttatta                                                                                                                                         <  1:215981/130‑1 (MQ=255)                                                                                                                                                                                                               gTATTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCCCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAAcc                                           <  1:438614/224‑1 (MQ=255)                                                                                                                                                                                                                 aTTGACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaagaatgtcac              <  1:234671‑M1/251‑15 (MQ=255)                                                                                                                                                                                                                    gcccGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGAc                                 <  1:176176/227‑1 (MQ=255)                                                                                                                                                                                                                    gACCGACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaagaatgtcacaaa           >  1:608728‑M1/1‑234 (MQ=255)                                                                                                                                                                                                                        gACACCATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCg                                                                                                                                      <  1:887623/124‑1 (MQ=255)                                                                                                                                                                                                                            ccATCATAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGa                                                                                                                                                                            >  1:634750/1‑82 (MQ=255)                                                                                                                                                                                                                             catcatAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaagaatgtcacaaaggatattat  <  1:295374‑M1/251‑27 (MQ=255)                                                                                                                                                                                                                             catcatAGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaagaatgtcacaaaggatattat  <  1:354398‑M1/251‑27 (MQ=255)                                                                                                                                                                                                                               tcataGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTgcgaagaatgtcacaaaggatattat  >  1:303773‑M1/1‑223 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     TTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCACGGCACATCGGCATATTTGGGGCCGATCGCAGTATTGACCGACACCATCATCGAAAAAATCCCGGGCTTGAATAAAGTGCATTTCTCCGCCGATGATTTGCAAAAACGCTTCGGACCGTTCGGCGAGCCGGTCACCGTCGGTTTTGTCATGGGGATTATTATCGGTATTTTGGCGGGCTATCCGTTAAAAGACATTTTGCAGTTAGCGGTAAAAACCGCTGCAGTGATGTTGTTAATGCCACGCGTCATTAAACCAATCATGGACGGTTTAACACCCATTGCCAAGCAAGCACGTA  >  1/1396945‑1397415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑