Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,394,918	A→G	L123L (TTA→TTG)	unknown →	Tagatose‑6‑phosphate kinase GatZ (EC 2.7.1.144) / PTS system, galactitol‑specific IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,394,918	0	A	G	100.0%	217.0 / NA	71	L123L (TTA→TTG)	unknown	Tagatose‑6‑phosphate kinase GatZ (EC 2.7.1.144) / PTS system, galactitol‑specific IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/34);  total (37/34)

TAATTGAAGCGACGTCTAATCAAGTGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTAGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTGAGACCCATCGCAAGGCATTTG  >  1/1394674‑1395162                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     tAATTGAAGCGACGTCTAATCAAGTGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATg                                                                                                                                                                                                                                                  >  1:72756/1‑249 (MQ=255)          cGACGTCTAATCAAGTGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGt                                                                                                                                                                                                                                         <  1:684308/249‑1 (MQ=255)              gTCTAATCAAGTGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTtgtg                                                                                                                                                                                                                                   >  1:659135/1‑251 (MQ=255)                  aaTCAAGTGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGGAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCtga                                                                                                                                                                                                                               >  1:444499/1‑251 (MQ=255)                         tGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATc                                                                                                                                                                                                                          <  1:201876/249‑1 (MQ=255)                                      cggTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTtcctc                                                                                                                                                                                                           <  1:887346/251‑1 (MQ=255)                                        gTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCg                                                                                                                                                                                                         >  1:503302/1‑251 (MQ=255)                                            taCCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTg                                                                                                                                                                                                     <  1:894756/251‑1 (MQ=255)                                                 gTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAAt                                                                                                                                                                                                 <  1:265802/250‑1 (MQ=255)                                                 gTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAAt                                                                                                                                                                                                 >  1:855392/1‑250 (MQ=255)                                                               gATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATCTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTtcctc                                                                                                                                                                                                           >  1:166637/1‑226 (MQ=255)                                                                aTTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCa                                                                                                                                                                                 >  1:362858/1‑251 (MQ=255)                                                                  tttAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGAc                                                                                                                                                                                                       <  1:105366/227‑1 (MQ=255)                                                                      aaGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTa                                                                                                                                                                           <  1:760120/251‑1 (MQ=255)                                                                                ttATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATgcgc                                                                                                                                                                      <  1:343631/246‑1 (MQ=255)                                                                                ttATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGccgccg                                                                                                                                                                 >  1:875649/1‑251 (MQ=255)                                                                                       aTAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGcgcctcaccacc                                                                                                                                                             <  1:870866/248‑9 (MQ=255)                                                                                       aTAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGaa                                                                                                                                                            <  1:440759/249‑1 (MQ=255)                                                                                                    tGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGa                                                                                                                                              <  1:771959/250‑1 (MQ=255)                                                                                                          ttCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGt                                                                                                                                                                                                                                         >  1:509956/1‑153 (MQ=255)                                                                                                          ttCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGc                                                                                                                                                                                                                                  <  1:570210/160‑1 (MQ=255)                                                                                                          ttCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCg                                                                                                                                               >  1:348747/1‑243 (MQ=255)                                                                                                          ttCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCaa                                                                                                                                       >  1:479220/1‑251 (MQ=255)                                                                                                               aaaGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGc                                                                                                                                  <  1:724841/251‑1 (MQ=255)                                                                                                                    gcgcATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAg                                                                                                                                                 >  1:221606/1‑231 (MQ=255)                                                                                                                     cgcATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCaaaa                                                                                                                             <  1:750508/250‑1 (MQ=255)                                                                                                                      gcATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCaaaaa                                                                                                                                    <  1:478161/242‑1 (MQ=255)                                                                                                                           attTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATc                                                                                                                           >  1:852284/1‑246 (MQ=255)                                                                                                                           attTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCta                                                                                                                      >  1:645990/1‑251 (MQ=255)                                                                                                                                aaGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATatca                                                                                                                 <  1:895370/251‑1 (MQ=255)                                                                                                                                     gCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATc                                                                                                                           >  1:360124/1‑236 (MQ=255)                                                                                                                                     gCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCgg                                                                                                             >  1:100704/1‑250 (MQ=255)                                                                                                                                        atcatcTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGt                                                                                                                                                                                                                                         >  1:371451/1‑123 (MQ=255)                                                                                                                                           atcTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGaa                                                                                                      >  1:826508/1‑251 (MQ=255)                                                                                                                                           atcTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGaa                                                                                                      >  1:775050/1‑251 (MQ=255)                                                                                                                                           atcTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGaa                                                                                                      >  1:192218/1‑251 (MQ=255)                                                                                                                                           atcTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGACTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTtc                                                                                                                                                                                                              >  1:261896/1‑147 (MQ=255)                                                                                                                                                  aCCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCtg                                                                                               <  1:832341/251‑1 (MQ=255)                                                                                                                                                  aCCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCt                                                                                                >  1:562861/1‑250 (MQ=255)                                                                                                                                                  aCCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCAGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAg                                                                                                     <  1:144490/245‑1 (MQ=255)                                                                                                                                                     gAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGt                                                                                                                                                                             <  1:51994/170‑1 (MQ=255)                                                                                                                                                            tgGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGggtgg                                                                                     <  1:783890/251‑1 (MQ=255)                                                                                                                                                               cAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGa                                                                                  <  1:598220/251‑1 (MQ=255)                                                                                                                                                                 aaaTGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAgg                                                                                <  1:598656/251‑1 (MQ=255)                                                                                                                                                                 aaaTGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAgg                                                                                >  1:857747/1‑251 (MQ=255)                                                                                                                                                                    tGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGgcgc                                                                             <  1:610805/251‑1 (MQ=255)                                                                                                                                                                         cGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATAc                                                                         <  1:369308/250‑1 (MQ=255)                                                                                                                                                                          ggCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCa                                                                       >  1:890149/1‑251 (MQ=255)                                                                                                                                                                           gCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCAt                                                                      >  1:237317/1‑251 (MQ=255)                                                                                                                                                                                aaTGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAAt                                                                 <  1:843661/251‑1 (MQ=255)                                                                                                                                                                                aaTGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCATCCTCCGACTGTAATCGCCAAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTGCCTGTGCCGGGTGGTGAGGCGCATGCCATTCAAt                                                                 <  1:74218/251‑1 (MQ=255)                                                                                                                                                                                 aTGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAAtg                                                                <  1:530695/251‑1 (MQ=255)                                                                                                                                                                                 aTGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAAtg                                                                <  1:470069/251‑1 (MQ=255)                                                                                                                                                                                    gCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCAt                                                                      >  1:703969/1‑242 (MQ=255)                                                                                                                                                                                         ggAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGggt                                                                                       >  1:348734/1‑220 (MQ=255)                                                                                                                                                                                           aaaaaGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGTTTGGTGAGGCGCATGCCATTCAATGTGTCGACGtt                                                      <  1:783341/251‑1 (MQ=255)                                                                                                                                                                                              aaGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTAc                                                    >  1:21246/1‑250 (MQ=255)                                                                                                                                                                                                gCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACca                                                  >  1:27133/1‑250 (MQ=255)                                                                                                                                                                                                 ccAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCGATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACcac                                                 <  1:117823/250‑1 (MQ=255)                                                                                                                                                                                                 ccAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACcac                                                 <  1:375551/250‑1 (MQ=255)                                                                                                                                                                                                           ggTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAg                                                                                                     >  1:184446/1‑188 (MQ=255)                                                                                                                                                                                                           ggTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCa                                      >  1:581957/1‑251 (MQ=255)                                                                                                                                                                                                                  gattATGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTc                                                                    >  1:269745/1‑214 (MQ=255)                                                                                                                                                                                                                      aTGCGAAAGCGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGTAAGCACCAACGaaa                             >  1:818904/1‑249 (MQ=255)                                                                                                                                                                                                                               cGGGTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTgaga                    <  1:887535/249‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTTACTAAAATTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCaaaaa                                                                                                                                    <  1:283758/134‑1 (MQ=255)                                                                                                                                                                                                                                            aaTTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTAt                         <  1:75727/231‑1 (MQ=255)                                                                                                                                                                                                                                             aTTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTGAGACCCATCGCAAGGCAtt    >  1:10095/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTTCATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTGAGACCCATCGCAAGGCAtt    >  1:615386/1‑251 (MQ=255)                                                                                                                                                                                                                                                cATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTGAGACCCATCGCAAGGCATTTg  >  1:248523/1‑250 (MQ=255)                                                                                                                                                                                                                                                cATTTGGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTGAGACCCATCGCAAGGCATTTg  >  1:304579/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     TAATTGAAGCGACGTCTAATCAAGTGAATCAATTCGGCGGTTATACCGGTATGAAACCGACAGATTTTAAAGCTTTCGTTTATCAGATAGCGGAAAAAGTGAATTTTCCGAAAGAGCGCATTATTTTAAGTGGCGATCATCTGGGACCGAATTGTTGGCAAAATGAAGCGGCTGAAATGGCGATGGAAAAAGCCAAAGCGTTGGTGATTGATTATGCGAAAGCGGGTTTTACTAAAATTCATTTAGATGCTTCAATGTCTTGTGCTGATGATCCGGTGCCCCTTCCTCCGACTGTAATCGCCGAATGCGCGGCAGAGTTATGCGCCGCCGTTGAAAGCGTGTTAAGCGAAGAGCAAAAAGCCAAAATCAGCTATATCATCGGCACCGAAGTACCTGTGCCGGGTGGTGAGGCGCATACCATTCAATGTGTCGACGTTACCACAGTGGCAAGCACCAACGAAACTATTGAGACCCATCGCAAGGCATTTG  >  1/1394674‑1395162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑