Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,393,650 A→C A23A (GCA→GCC)  unknown → Tagatose 1,6‑bisphosphate aldolase (EC 4.1.2.40)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,393,6500AC100.0% 88.0 / NA 30A23A (GCA→GCC) unknownTagatose 1,6‑bisphosphate aldolase (EC 4.1.2.40)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/10);  total (20/10)

TTAGAAAATACGATTTGTAATGTTTTGTGATATTGATCACAACTTTCATAAGTTTTCATATTCAAAATTATTTTATTATGTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCAATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCTGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAACCGGCTATGACAAAACCAAAATCGGACGTTTTCCCGATT  >  1/1393443‑1393882
                                                                                                                                                                                                               |                                                                                                                                                                                                                                        
ttAGAAAATACGATTTGTAATGTTTTGTGATATTGATCACAACTTTCATAAGTTTTCATATTCAAAATTATTTTATTATGTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAgg                                                                                                                                                                                               <  1:64221/251‑1 (MQ=255)
              ttGTAATGTTTTGTGATATTGATCACAACTTTCATAAGTTTTCATATTCAAAATTATTTTATTATGTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGagcag                                                                                                                                                                                 >  1:410683/1‑251 (MQ=255)
                     gTTTTGTGATATTGATCACAACTTTCATAAGTTTTCATATTCAAAATTATTTTATTATGTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGt                                                                                                                                                                                              <  1:151380/231‑1 (MQ=255)
                                                                        ttattaTGTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCt                                                                                                                                 >  1:544220/1‑241 (MQ=255)
                                                                               gTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGa                                                                                                                >  1:38555/1‑251 (MQ=255)
                                                                                        aTTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGa                                                                                                                                                                                                                                     >  1:416760/1‑125 (MQ=255)
                                                                                        aTTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATc                                                                                                                                                                                                                                   <  1:253797/127‑1 (MQ=255)
                                                                                        aTTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATg                                                                                                                                                                                      >  1:108290/1‑172 (MQ=255)
                                                                                        aTTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAAtt                                                                                                       >  1:644008/1‑251 (MQ=255)
                                                                                        aTTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAAtt                                                                                                       >  1:830126/1‑251 (MQ=255)
                                                                                                   atCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAAttt                                                                                                      <  1:124188/241‑1 (MQ=255)
                                                                                                          gTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCggg                                                                                     >  1:776800/1‑251 (MQ=255)
                                                                                                          gTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCggg                                                                                     >  1:758127/1‑251 (MQ=255)
                                                                                                          gTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCggg                                                                                     >  1:307733/1‑251 (MQ=255)
                                                                                                          gTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCggg                                                                                     >  1:123872/1‑251 (MQ=255)
                                                                                                                       atCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGagcag                                                                        >  1:265059/1‑251 (MQ=255)
                                                                                                                                         aGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGTGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATg                                                      >  1:470796/1‑251 (MQ=255)
                                                                                                                                          gATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAg                                                                                                                                                                                                                                 <  1:139498/79‑1 (MQ=255)
                                                                                                                                                 atatTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGAATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGa                                              >  1:581748/1‑251 (MQ=255)
                                                                                                                                                 atatTAAAACAAAAACGGTTGGAAAAATTAGACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTGTAAAAGGTTAGTGACCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCgg                                                                <  1:829444/233‑1 (MQ=255)
                                                                                                                                                  tatTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGaa                                             >  1:520109/1‑251 (MQ=255)
                                                                                                                                                  tatTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGaa                                             >  1:667666/1‑251 (MQ=255)
                                                                                                                                                       aaaCAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAAc                                        <  1:848808/251‑1 (MQ=255)
                                                                                                                                                        aaCAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGc                                                                                                                                                                                                                 <  1:549080/81‑1 (MQ=255)
                                                                                                                                                         aCAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAACCg                                      >  1:856514/1‑251 (MQ=255)
                                                                                                                                                                  gTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTg                                                                                                                                                                                                     >  1:385539/1‑83 (MQ=255)
                                                                                                                                                                         aaaTTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGAAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAACCGGCTATGACAAAACCaa                      >  1:843616/1‑251 (MQ=255)
                                                                                                                                                                              ataCGGTAAAGAGCGAATTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAACCGGCTATGACAAAACCAAAATCg                 >  1:759016/1‑251 (MQ=255)
                                                                                                                                                                                             aaTTTTTCGCGCTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCCGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAACCGGCTATGACAAAACCAAAATCGGACGTTTTCCCGAtt  <  1:140033/251‑1 (MQ=255)
                                                                                                                                                                                                      cgcTTTAGCCATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAgg                                                                                                                                                               <  1:525762/85‑1 (MQ=255)
                                                                                                                                                                                                               |                                                                                                                                                                                                                                        
TTAGAAAATACGATTTGTAATGTTTTGTGATATTGATCACAACTTTCATAAGTTTTCATATTCAAAATTATTTTATTATGTATTATTAATTCAAAACATATCGAAAGTTTTAAGTGAATATCGAAAGTAGGAGAAAAAGATGACAATATTAAAACAAAAACGGTTGGAAAAATTATACGGTAAAGAGCGAATTTTTCGCGCTTTAGCAATTGATCAGCGCGGCGCGTTAAAGCGGATGCTGGGTGAAGAGGTAACGGATGAGCAGCTTCAGATTTTTAAAAGGTTAGTGTCCGAGAACTTGACGACACGGGCTTCAGCCATTTTGTTGGATCTGGAATTTGGCTGGCAGGCAGCGGGATTAAAAGAGCAGCATTGCGGTTTAATTATGGCTTACGAAAAAACCGGCTATGACAAAACCAAAATCGGACGTTTTCCCGATT  >  1/1393443‑1393882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑