Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,371,086	C→T	intergenic (‑447/+414)	unknown ← / ← unknown	hypothetical protein/Thiol:disulfide oxidoreductase associated with MetSO reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,371,086	0	C	T	100.0%	88.7 / NA	32	intergenic (‑447/+414)	unknown/unknown	hypothetical protein/Thiol:disulfide oxidoreductase associated with MetSO reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

ATCCTTATAACTATCAAATAGTTCGATTTCTTTGGATGGTTTTCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGCAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTGCGTAGATAACAAATAAAAGTTCTTTATCAAATAAAAAATTAGTATAACTATACCTTATTTCAAAATGAAG  >  1/1370841‑1371295                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  aTCCTTATAACTATCAAATAGTTCGATTTCTTTGGATGGTTTTCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAggt                                                                                                                                                                                                              <  1:644796/251‑1 (MQ=255) aTCCTTATAACTATCAAATAGTTCGATTTCTTTGGATGGTTTTCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAggt                                                                                                                                                                                                              <  1:493623/251‑1 (MQ=255)   ccTTATAACTATCAAATAGTTCGATTTCTTTGGATGGTTTTCAAGGCACTATAACTGTATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAggt                                                                                                                                                                                                              >  1:207292/1‑249 (MQ=255)           cTATCAAATAGTTCGATTTCTTTGGATGGTTTTCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGtttattt                                                                                                                                                                                                    <  1:202339/251‑1 (MQ=255)                                       gTTTTCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAggt                                                                                                                                                                                                              >  1:670643/1‑213 (MQ=255)                                        ttttCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAAc                                                                                                                                                                        <  1:462281/250‑1 (MQ=255)                                        ttttCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACt                                                                                                                                                                       <  1:727808/251‑1 (MQ=255)                                                      taactataTGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGt                                                                                                                                                                     <  1:834970/239‑1 (MQ=255)                                                                       aCTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACt                                                                                                                                                                       >  1:91544/1‑220 (MQ=255)                                                                                            gTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACt                                                                                                                                                                       <  1:808402/199‑1 (MQ=255)                                                                                                         gTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGat                                                                                                                                                              <  1:703871/195‑1 (MQ=255)                                                                                                                         gtaaAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGAt                                                                                                                                                      >  1:389162/1‑187 (MQ=255)                                                                                                                                    aTACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGCTAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGAtata                                                                           >  1:758282/1‑251 (MQ=255)                                                                                                                                    aTACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGt                                                                                                                                               >  1:618154/1‑183 (MQ=255)                                                                                                                                          agaTCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTGc                                                                       <  1:808041/249‑1 (MQ=255)                                                                                                                                            aTCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTGCGt                                                                     <  1:873730/249‑1 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAggt                                                                                                                                                                                                              >  1:583202/1‑111 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTg                                                                                                                                                                      <  1:607989/151‑1 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATaa                                                                                                                                                    >  1:287519/1‑169 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATatgat                                                                                                                               >  1:741156/1‑190 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGat                                                                              <  1:538900/239‑1 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTGCGTAGa                                                                  >  1:595933/1‑251 (MQ=255)                                                                                                                                             tCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGCATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAAc                                                                                                                                       >  1:870771/1‑182 (MQ=255)                                                                                                                                                                             aaTTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGt                                                                                                                                               >  1:656502/1‑142 (MQ=255)                                                                                                                                                                                      aGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAAc                                                                                                                                       <  1:317342/141‑1 (MQ=255)                                                                                                                                                                                      aGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGatat                                                                                                                          >  1:331543/1‑154 (MQ=255)                                                                                                                                                                                                             cAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTGCGTAGATAACAAATAAAAGTTCTTTATCAAATAAAAAATTAGTATAACTATACCTTATTTCAAAATGAag  >  1:266754/1‑251 (MQ=255)                                                                                                                                                                                                                    tttATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAAc                                                                                                                                                                        <  1:179965/78‑1 (MQ=255)                                                                                                                                                                                                                    tttATGAACAATGTGACTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATatg                                                                                                                                 >  1:143898/1‑117 (MQ=255)                                                                                                                                                                                                                                    cTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATatgat                                                                                                                               <  1:232203/103‑1 (MQ=255)                                                                                                                                                                                                                                    cTAAAAAGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTg                                                                        >  1:431436/1‑158 (MQ=255)                                                                                                                                                                                                                                         aaGTTATTCTACGTAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGtttatttt                                                                                                        >  1:391071/1‑121 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  ATCCTTATAACTATCAAATAGTTCGATTTCTTTGGATGGTTTTCAAGGCACTATAACTATATGTACAATTACTGTCAAAAATGATTTTAAGGTTATCGGCACAAGTGCACTGGTAAGTAAGTAAAACTACAATACAGAGATCTGTAAACAGATCGCTTATCAAAATTTGATAAATTGTGATAGCTAGAGGAGTATTTATTAAAACAGCGTATTTATGAACAATGTGACTAAAAAGTTATTCTACGCAAGGTTGGTTTATTTACTTAAGCCGATTTTGCAAAATATTAACTGTTGAAGATAATATGATAATGAGTTATCTAACTATATGATGATATACCAAAATGGTTTATTTTATTTTGATGTAGAAATTGAATTTGATATAGTGCGTAGATAACAAATAAAAGTTCTTTATCAAATAAAAAATTAGTATAACTATACCTTATTTCAAAATGAAG  >  1/1370841‑1371295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑