Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,370,003	T→C	intergenic (‑767/‑68)	unknown ← / → unknown	Fructose repressor FruR, LacI family/FIG00699498: hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,370,003	0	T	C	100.0%	60.7 / NA	23	intergenic (‑767/‑68)	unknown/unknown	Fructose repressor FruR, LacI family/FIG00699498: hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/11);  total (12/11)

GTTTAAAGGTTATTCATATTATTGACCGTTATAAAATGACACATAAGATTGCTCATGAAGTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCTGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAATTTTTAAATATTAGATTTGTTGAAGACGGACGCAAAGAT  >  1/1369826‑1370244                                                                                                                                                                                  |                                                                                                                                                                                                                                                  gTTTAAAGGTTATTCATATTATTGACCGTTATAAAATGACACATAAGATTGCTCATGAAGTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGCATCTAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTa                                                                                                                                                                          >  1:865589/1‑251 (MQ=255)             ttCATATTATTGACCGTTATAAAATGACACATAAGATTGCTCATGAAGTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAgtt                                                                                                                                                              <  1:82868/251‑1 (MQ=255)                atatTATTGACCGTTATAAAATGACACATAAGATTGCTAATGAAGTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGATTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCATATAGTAAAATATTGTTActgcctcttataaa                                                                                                                                                            >  1:10590/1‑237 (MQ=255)                                         acaTAAGATTGCTCATGAAGTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCATGAAATAAAGAATTATAATAATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTAc                                                                                                                                                    >  1:89922/1‑233 (MQ=255)                                                            gTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACtt                                                                                                                      >  1:249511/1‑244 (MQ=255)                                                                     gCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACAt                                                                                                                                                                                         <  1:257481/168‑1 (MQ=255)                                                                          aatgtAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAatat                                                                                                                                                                               <  1:429808/169‑1 (MQ=255)                                                                          aaTTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAAt                                                                                                  >  1:340929/1‑250 (MQ=255)                                                                                                                          aatGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGc                                                               <  1:156865/237‑1 (MQ=255)                                                                                                                               atatAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAaatttaattt                                                                                                                                                                                                   >  1:244424/1‑100 (MQ=255)                                                                                                                                      gtAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAATTTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAAtttt                                     >  1:255244/1‑251 (MQ=255)                                                                                                                                                           gAAATAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAaat                                                                                                                                                                                                          >  1:387129/1‑65 (MQ=255)                                                                                                                                                              aTAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACa                                                                                                                                                                        <  1:45197/96‑1 (MQ=255)                                                                                                                                                              aTAAAGTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGc                                                               <  1:773409/201‑1 (MQ=255)                                                                                                                                                                   gTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATc                                                                                                                                                                                                              <  1:586482/53‑1 (MQ=255)                                                                                                                                                                   gTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAatat                                                                                                                                                                               <  1:342395/84‑1 (MQ=255)                                                                                                                                                                   gTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTAc                                                                                                                                                    <  1:847173/111‑1 (MQ=255)                                                                                                                                                                   gTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGc                                                          <  1:697630/201‑1 (MQ=255)                                                                                                                                                                   gTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAATTTTTa                                   >  1:357618/1‑224 (MQ=255)                                                                                                                                                                   gTATTATAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAATTTTTAAATATTAGATTTGTTGAAgacggacg         >  1:852500/1‑250 (MQ=255)                                                                                                                                                                     attatAATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAATTTTTAAATATTAGATTTGTTGAAGACGGACGCaa      >  1:133826/1‑251 (MQ=255)                                                                                                                                                                         taATGATGCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAATTTTTAAATATTAGATTTGTTGAAGACGGACGCAAAGAt  <  1:420159/251‑1 (MQ=255)                                                                                                                                                                           atgatgCCGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGcac                                               >  1:717722/1‑204 (MQ=255)                                                                                                                                                                                  |                                                                                                                                                                                                                                                  GTTTAAAGGTTATTCATATTATTGACCGTTATAAAATGACACATAAGATTGCTCATGAAGTTGAGGGTGCATTAATTTAGGCTTATCCAGGATTTTCAAATATTCAAAGTGGGGCGGGAATAATGAATATAGTGTAATAAATGCTTCTCATCTTGAAATAAAGTATTATAATGATGCTGCGCGCGATAAATTAACCACTTTCAGCGCGATAAATCAAATTTAATTTTATTTCACATAGTAAAATATTGTTACAAAAAGAAGTTTGTTTATTACAGAGAAATTCCTTTTTAGTCGGTTTAACTTCGGGTATTTTAAGAAAAAATCTCTGTAATTTTTACAGAATCAAAATTGATAACGCTACGCCAATTTTGCACACCGAATTTTTAAATATTAGATTTGTTGAAGACGGACGCAAAGAT  >  1/1369826‑1370244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑