| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,366,770 | A→G | N197N (AAT→AAC) | unknown ← | Homoserine O‑acetyltransferase (EC 2.3.1.31) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,366,770 | 0 | A | G | 96.5% | 162.3 / ‑6.4 | 58 | N197N (AAT→AAC) | unknown | Homoserine O‑acetyltransferase (EC 2.3.1.31) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (28/28); minor base T (0/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTATAAATCCAACGCGCGTAATAAATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCATTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAGGAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCCAGCAGCGCTTTTTGCACTTTGATGATGTCTTGCACG > 1/1366526‑1367007 | cGTATAAATCCAACGCGCGTAATAAATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTg < 1:808745/251‑1 (MQ=255) aaTCCAACGCGCGTAATAAATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAAttt > 1:366393/1‑251 (MQ=255) gcgcgTAATAAATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGtt > 1:769080/1‑251 (MQ=255) taataaATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTtcatca > 1:480929/1‑251 (MQ=255) taataaATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTtcatc < 1:165592/250‑1 (MQ=255) cttcgatgccaacagttatttGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTtcatca > 1:696928/20‑251 (MQ=255) aTGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑At > 1:416247/1‑236 (MQ=255) aaTAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGgcg > 1:546859/1‑250 (MQ=255) tGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACg < 1:102990/251‑1 (MQ=255) tGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACg < 1:530176/251‑1 (MQ=255) aaGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATTACCGCTTGGCGCATGACGTGATTAAAAc < 1:252185/250‑1 (MQ=255) ccAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGc < 1:755172/250‑1 (MQ=255) gTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCt > 1:280318/1‑239 (MQ=255) gTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAg > 1:522718/1‑251 (MQ=255) ccTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTg < 1:541227/183‑1 (MQ=255) ccTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAAc > 1:499817/1‑229 (MQ=255) tAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGa < 1:105272/251‑1 (MQ=255) gCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGgcgc > 1:205208/1‑205 (MQ=255) gCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAg > 1:365289/1‑246 (MQ=255) tAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAagag > 1:231385/1‑250 (MQ=255) aaaTAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCa > 1:367384/1‑251 (MQ=255) aaaTAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCa > 1:884019/1‑251 (MQ=255) aaTAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCAt < 1:44743/251‑1 (MQ=255) gATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGa < 1:729237/249‑1 (MQ=255) gATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGAAGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGAt < 1:616523/250‑1 (MQ=255) aTTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGAtt < 1:886831/250‑1 (MQ=255) aTTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGAtt < 1:390784/250‑1 (MQ=255) ccACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAAc > 1:770944/1‑250 (MQ=255) aCTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACcac < 1:523404/251‑1 (MQ=255) aCTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACcac < 1:82874/251‑1 (MQ=255) aCTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACcac < 1:469426/251‑1 (MQ=255) cTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑At < 1:791868/168‑1 (MQ=255) cccTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGTGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCTCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGatat > 1:865269/1‑247 (MQ=255) cTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAag < 1:325040/218‑1 (MQ=255) cTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGgaga < 1:763737/242‑1 (MQ=255) gCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGa > 1:377398/1‑251 (MQ=255) cAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGa < 1:782787/250‑1 (MQ=255) aCCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGTGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATaatca < 1:431481/250‑1 (MQ=255) cTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAAtt > 1:459817/1‑130 (MQ=255) ttGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACcac < 1:192475/215‑1 (MQ=255) gtggCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTggg < 1:856509/250‑1 (MQ=255) tCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCG‑‑GGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCAt < 1:460132/250‑1 (MQ=255) gCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGc > 1:511485/1‑250 (MQ=255) gCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAgg > 1:563148/1‑251 (MQ=255) gTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAACGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATAc > 1:837097/1‑237 (MQ=255) gTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAAc < 1:631276/184‑1 (MQ=255) caatgcggtgttaatctgccacgcgCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAGGAAc < 1:884612/225‑1 (MQ=255) gTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAg > 1:495920/1‑234 (MQ=255) aTACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAAcc > 1:804326/1‑169 (MQ=255) tGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAGGAACCGCCGATAATGGCGTGTAAATg > 1:207859/1‑250 (MQ=255) aCCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAag > 1:34959/1‑143 (MQ=255) gAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCg < 1:798764/185‑1 (MQ=255) ggCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATaat > 1:214537/1‑170 (MQ=255) gggctaaccttttgccggggCGCTCGCCGTAATAATAACCGCCTTTATT‑AAATTTGGG‑ATCGTCAACCCCCGCTGGGCGCAGGCCGTGATAAAACCCGAGGGCTTCCGCGCTAAAGGAAGGGAAAGAAAATAAATAACCCAAATGGGAAACAAAACCGGGAAAATCAATC‑CCCCTTGGGTTTGCCGGCATACCGCAAAAGGAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCcagcag < 1:293946/233‑1 (MQ=255) ccTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAGGAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCCAGCAGCGCTTTTTg > 1:330447/1‑251 (MQ=255) cccTCGCCATCAAAATAATCGCCTTTATT‑AAATTT‑GGTATCTTTCATCCCCGCTTGGCGCATGCCGTAATAAAACCCGATGTCTTCCGCGCTCAAGAAAAGGGAAGAGAATAATTTACCCACATTGAAGACAAAATCGGAATAACCACTCGCCTATTGGT‑TCGCCGGCATCCCGCAAAAGAAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCCAGCAGCGCTTTTTGCACTTtgatga < 1:31195/248‑1 (MQ=255) taataataATCGCCGTTATTAAAATTT‑GG‑ATCGTTCACCCCCGTTGGGCCAATGACGTGATAAAACCCGAGGGCTTCCGCGCTAAAGGAAAGGGAAGAGCATAGATAACCCACATGGGAGACGAAAGCGGAATAATCAATCCCCCATTGGG‑TTGCCTGCATCCCGCAAAAGGAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCCAGCAGCGCTTTTTGCACTTTGATGATGTCTTGCAc < 1:507849/249‑1 (MQ=255) cATAATAATCGCCGTTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAGGAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCCAGCAGCGCTTTTTGCACTTTGATGATGTCTTGCACg > 1:332961/1‑251 (MQ=255) | CGTATAAATCCAACGCGCGTAATAAATGCAAATAACTGTTGGCATCGAAGCGCGCCAGAAATTTTTGTCCTTGATAGCTTAAATAGGATTCCACTTGAAAATAGTCGCCCTGCAAATGACCTTCCGCTTTGGTGGCGCGTCCGAAGGCTTTGCTGAGTTGGACATCGGTGCGATACGTGAGCATACCGAGCATACGGGCGATGGCTAAACCTTTGTCCGGGCGCTCGCCGTCATAATAATCGCCATTATTGAAATTT‑GG‑ATCGTTCATCACCGCTTGGCGCATGACGTGATTAAAACCGATGGCTTCCGCGCTCAAGGAAAGGGAAGAGCATAGATTAACCACATTGGAGACGAAATCGGGATAATCAATCGCCCATTGGG‑TTGCCTGCATACCGCCAAAGGAACCGCCGATAATGGCGTGTAAATGCGGAATTTCAAGAAAATCCAGCAGCGCTTTTTGCACTTTGATGATGTCTTGCACG > 1/1366526‑1367007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |