| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,363,407 | C→T | A63V (GCC→GTC) | unknown → | 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase (EC 2.3.1.117) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,363,407 | 0 | C | T | 98.3% | 165.7 / NA | 59 | A63V (GCC→GTC) | unknown | 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase (EC 2.3.1.117) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (20/38); minor base G (1/0); total (21/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAACGACGAACACCCG‑‑CCCCCCCTTTTTACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGCCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGATACT > 1/1363167‑1363645 | gCAACGACGAACACCCGCCCCCCCCCTTTTTACTAACATTGATATAAGAAGCAAAAAGTCAAAATTACAACAACTTATTTAAAACGCTTTTTAAAAAAGCACTGAAAATAAAACAAAAAACATTGACGGACAAACCCGTGCGGCGGGTGGAGAGGGGATTGAAAGGTTGGGCAGCGGGAAGAATCGGGTTGGCGGAAAAAAAGACGGGGGAAGGGGGGA‑TCACCAAAGGGTGAAAAAAACGGGCTTTTTAt > 1:604330/1‑251 (MQ=255) cccccccccTTTTTACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATc < 1:310267/233‑1 (MQ=255) cccccccccTTTTTACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGa < 1:781560/248‑1 (MQ=255) cccccccccTTTTTACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGa > 1:422272/3‑250 (MQ=255) cccTTTTTACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCaa > 1:290306/1‑251 (MQ=255) ttACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACttgtt > 1:801798/1‑251 (MQ=255) cTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGAc < 1:97976/251‑1 (MQ=255) aaCATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACgg > 1:98854/1‑251 (MQ=255) cATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTg > 1:787487/1‑251 (MQ=255) ttGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCa > 1:849496/1‑251 (MQ=255) gATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTa < 1:650403/210‑1 (MQ=255) ataGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAAc < 1:176900/251‑1 (MQ=255) cAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAAtac < 1:48168/250‑1 (MQ=255) cAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAAtac < 1:461081/250‑1 (MQ=255) cAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAAtac < 1:45454/250‑1 (MQ=255) aTTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAAtac < 1:668210/239‑1 (MQ=255) aacTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGAc < 1:74880/212‑1 (MQ=255) aacTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGaaa > 1:252554/1‑251 (MQ=255) acTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAAt < 1:358175/251‑1 (MQ=255) cTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACttg < 1:775856/192‑1 (MQ=255) tGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCgaag < 1:135928/251‑1 (MQ=255) tGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCcaac < 1:70606/251‑1 (MQ=255) aaaTTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCACCTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCcaacaa > 1:428396/1‑251 (MQ=255) aaaTTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCcaacaa > 1:606597/1‑251 (MQ=255) aaTTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTAGCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCcaac < 1:436345/248‑1 (MQ=255) ccATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGAc < 1:246702/149‑1 (MQ=255) ttGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGAc < 1:323645/161‑1 (MQ=255) tGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGc < 1:231087/251‑1 (MQ=255) tGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGc < 1:659719/251‑1 (MQ=255) gTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACg < 1:220889/139‑1 (MQ=255) gTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGAtt > 1:405759/1‑205 (MQ=255) gTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGt > 1:557019/1‑250 (MQ=255) gTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGt > 1:97829/1‑250 (MQ=255) aCCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTAc < 1:185978/251‑1 (MQ=255) gcggTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAAAGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTaa > 1:573655/1‑251 (MQ=255) gTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAAtac < 1:65346/156‑1 (MQ=255) ttGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAgg < 1:396733/250‑1 (MQ=255) aTTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTAc > 1:220454/1‑227 (MQ=255) cTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAAc < 1:617544/250‑1 (MQ=255) gCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCgaagaa > 1:769903/1‑170 (MQ=255) gCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCg < 1:389108/153‑1 (MQ=255) gCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACaagaa < 1:621412/182‑1 (MQ=255) gTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGc < 1:6166/194‑1 (MQ=255) gTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTaaa > 1:331903/1‑214 (MQ=255) gCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAata < 1:699481/237‑1 (MQ=255) tGGGTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTa > 1:460832/1‑199 (MQ=255) gggTGACTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTTCCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGc < 1:32376/248‑1 (MQ=255) aCTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGc < 1:886690/157‑1 (MQ=255) cTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGc < 1:657505/156‑1 (MQ=255) cTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTAtg < 1:784541/219‑1 (MQ=255) cTCACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCAc > 1:193773/1‑250 (MQ=255) cACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTAtgt < 1:327124/218‑1 (MQ=255) cACCAATGGTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCgg > 1:123578/1‑227 (MQ=255) ggTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGc < 1:254182/134‑1 (MQ=255) ggTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGAt > 1:71514/1‑251 (MQ=255) gTTGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGAt < 1:683377/250‑1 (MQ=255) ttGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGATAc < 1:805651/251‑1 (MQ=255) tGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGATACt < 1:230876/251‑1 (MQ=255) tGAAAAAAGCGGTCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGATACt < 1:318497/251‑1 (MQ=255) | GCAACGACGAACACCCG‑‑CCCCCCCTTTTTACTAACATTGATATAGGAAGCAAAATGTCAAATTTACAACAACTTATTGAAAACGCTTTTGAAAAACGCACTGAAATTACACCAAAAACCATTGACGTACAAACCCGTGCGGCGGTTGAAGAGGTGATTGAAGGCTTGGACAGCGGCAAGTATCGTGTTGCCGAAAAAATCGAC‑GGCGAATGGGTGACTCACCAATGGTTGAAAAAAGCGGCCTTGTTATCTTTCCGTATTAACGACAATCAACTTGTTGACGGTGCAGAAACCAAATACTACGACAAAGTCCCATTGAAATTTGCCGATTACACCGAAGAACGTTTCCAACAAGAAGGCTTCCGTGTAGTGCCGTCTGCTACCGTACGTAAAGGCGCATATATTGCTAAAAACTGCGTGTTAATGCCGTCTTATGTGAATATCGGCGCTCACGTGGGCGAAGGCACCATGGTAGATACT > 1/1363167‑1363645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |