Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,359,588 C→A S38* (TCG→TAG)  unknown → Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,359,5880CA100.0% 222.8 / NA 77S38* (TCG→TAG) unknownChorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/36);  total (41/36)

CTGTTGCAACTGCTGGCGAAACGGCAACAGGTGGTGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATCGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATATGCTGCTGGCGGATTTCACT  >  1/1359342‑1359813
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 
cTGTTGCAACTGCTGGCGAAACGGCAACAGGTGGTGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTa                                                                                                                                                                                                                               >  1:412207/1‑251 (MQ=255)
  gTTGCAACTGCTGGCGAAACGGCAACAGGTGGTGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTAt                                                                                                                                                                                                                              >  1:665033/1‑250 (MQ=255)
   ttGCAACTGCTGGCGAAACGGCAACAGGTGGTGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCg                                                                                                                                                                                                                            <  1:46201/251‑1 (MQ=255)
                       gCAACAGGTGGTGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACt                                                                                                                                                                                                         <  1:771804/250‑1 (MQ=255)
                             ggtggtGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAg                                                                                                                                                                                                   >  1:124926/1‑250 (MQ=255)
                                        aaGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCg                                                                                                                                                                                       >  1:56698/1‑251 (MQ=255)
                                        aaGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCg                                                                                                                                                                                       >  1:220995/1‑251 (MQ=255)
                                                    tGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAggg                                                                                                                                                                           <  1:613508/251‑1 (MQ=255)
                                                            cAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCgg                                                                                                                                                                     >  1:807584/1‑249 (MQ=255)
                                                                    gTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTg                                                                                                                                                                                                                          <  1:114620/188‑1 (MQ=255)
                                                                       gCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGaaa                                                                                                                                                        >  1:274073/1‑251 (MQ=255)
                                                                        cccATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGGAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAAt                                                                                                                                                       >  1:455753/1‑251 (MQ=255)
                                                                          cATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGt                                                                                                                                                     >  1:94153/1‑251 (MQ=255)
                                                                           aTTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGt                                                                                                                                                     >  1:390939/1‑250 (MQ=255)
                                                                           aTTATGCACCACAGCGTGAGGCGGAAATGTCGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGt                                                                                                                                                     >  1:24698/1‑250 (MQ=255)
                                                                              aTGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGc                                                                                                                                                                    >  1:220236/1‑232 (MQ=255)
                                                                              aTGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTgg                                                                                                                                                 >  1:300745/1‑251 (MQ=255)
                                                                              aTGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTgg                                                                                                                                                 >  1:654811/1‑251 (MQ=255)
                                                                              aTGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAATTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTgg                                                                                                                                                 >  1:441676/1‑251 (MQ=255)
                                                                                 caccacAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAg                                                                                                                                               <  1:121490/250‑1 (MQ=255)
                                                                                        gCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTc                                                                                                                                                                                            >  1:401318/1‑198 (MQ=255)
                                                                                        gCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGa                                                                                                                                       >  1:760487/1‑251 (MQ=255)
                                                                                         cGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGa                                                                                                                                          <  1:35212/247‑1 (MQ=255)
                                                                                                  gAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGc                                                                                                                                              <  1:639559/234‑1 (MQ=255)
                                                                                                                  aGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTg                                                                                                              >  1:852478/1‑250 (MQ=255)
                                                                                                                  aGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTg                                                                                                              >  1:554477/1‑250 (MQ=255)
                                                                                                                   gCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGgcgc                                                                                                                               <  1:188625/232‑1 (MQ=255)
                                                                                                                                  gCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGt                                                                                             >  1:411416/1‑251 (MQ=255)
                                                                                                                                   cAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTgg                                                                                              <  1:853657/249‑1 (MQ=255)
                                                                                                                                       tGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtg                                                                                        <  1:384612/251‑1 (MQ=255)
                                                                                                                                       tGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtg                                                                                        <  1:748397/251‑1 (MQ=255)
                                                                                                                                        ggggTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGgcgcgcgc                                                                                                                           >  1:106385/1‑215 (MQ=255)
                                                                                                                                          ggTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtgtg                                                                                      <  1:563225/250‑1 (MQ=255)
                                                                                                                                          ggTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGAGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtgtg                                                                                      <  1:305208/250‑1 (MQ=255)
                                                                                                                                           gTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGc                                                                                                                                              >  1:785485/1‑193 (MQ=255)
                                                                                                                                                ctcCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCg                                                                                                                                                                                                                            >  1:801715/1‑110 (MQ=255)
                                                                                                                                                ctcCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCaa                                                                                                                                                                                                            <  1:86392/126‑1 (MQ=255)
                                                                                                                                                ctcCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCt                                                                                                                                                   >  1:32970/1‑183 (MQ=255)
                                                                                                                                                ctcCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGTGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtg                                                                                        <  1:182067/242‑1 (MQ=255)
                                                                                                                                                          gATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtg                                                                                        <  1:814796/232‑1 (MQ=255)
                                                                                                                                                          gATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCaa                                                                     >  1:235864/1‑251 (MQ=255)
                                                                                                                                                          gATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCaa                                                                     >  1:186943/1‑251 (MQ=255)
                                                                                                                                                            ttGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATTTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGTCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAaca                                                                   <  1:83709/251‑1 (MQ=255)
                                                                                                                                                               aaGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACg                                                                 <  1:845461/250‑1 (MQ=255)
                                                                                                                                                                  aTGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTGTTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTg                                                                                                                                    >  1:893811/1‑180 (MQ=255)
                                                                                                                                                                   tgtgCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCtg                                                                                  <  1:493037/229‑1 (MQ=255)
                                                                                                                                                                    gtgCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtg                                                                                        <  1:701793/222‑1 (MQ=255)
                                                                                                                                                                    gtgCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCg                                                           >  1:318145/1‑251 (MQ=255)
                                                                                                                                                                    gtgCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCg                                                           >  1:837721/1‑251 (MQ=255)
                                                                                                                                                                    gtgCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCg                                                           >  1:251930/1‑251 (MQ=255)
                                                                                                                                                                    gtgCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGAGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCg                                                           >  1:441052/1‑251 (MQ=255)
                                                                                                                                                                         aCGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGAt                                                      <  1:832000/251‑1 (MQ=255)
                                                                                                                                                                         aCGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGAt                                                      <  1:491046/251‑1 (MQ=255)
                                                                                                                                                                           gccgcATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtg                                                                                        <  1:812182/215‑1 (MQ=255)
                                                                                                                                                                           gccgcATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCg                                                    >  1:734336/1‑251 (MQ=255)
                                                                                                                                                                              gcATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGaa                                                  >  1:606258/1‑250 (MQ=255)
                                                                                                                                                                               cATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAAc                                                 <  1:91922/250‑1 (MQ=255)
                                                                                                                                                                                   aTGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCt                                             >  1:43525/1‑250 (MQ=255)
                                                                                                                                                                                      cGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACg                                                                                                                                                                                                                  <  1:71221/82‑1 (MQ=255)
                                                                                                                                                                                        tGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAAcc                                       <  1:147545/251‑1 (MQ=255)
                                                                                                                                                                                        tGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAAcc                                       <  1:351911/251‑1 (MQ=255)
                                                                                                                                                                                          aaTCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGcc                                                                              >  1:766571/1‑210 (MQ=255)
                                                                                                                                                                                          aaTCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCtt                                     >  1:99563/1‑251 (MQ=255)
                                                                                                                                                                                           aTCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTa                                    <  1:721106/251‑1 (MQ=255)
                                                                                                                                                                                            tCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTAt                                   >  1:570167/1‑251 (MQ=255)
                                                                                                                                                                                            tCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTAt                                   >  1:371669/1‑251 (MQ=255)
                                                                                                                                                                                            tCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGCGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGCGGCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTAt                                   >  1:342742/1‑251 (MQ=255)
                                                                                                                                                                                             ccTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTAtt                                  <  1:716440/251‑1 (MQ=255)
                                                                                                                                                                                                            aTCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATAtgc                   <  1:359939/251‑1 (MQ=255)
                                                                                                                                                                                                              cAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGCTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGGGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATAtg                    >  1:773334/1‑248 (MQ=255)
                                                                                                                                                                                                               aaTTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATAtgctgc                <  1:674571/251‑1 (MQ=255)
                                                                                                                                                                                                               aaTTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATAtgctgc                <  1:620135/251‑1 (MQ=255)
                                                                                                                                                                                                                     gCTTTAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAAtt                                                                                         <  1:15224/172‑1 (MQ=255)
                                                                                                                                                                                                                        ttAAAACCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAacac                                                                  <  1:162659/192‑1 (MQ=255)
                                                                                                                                                                                                                             aCCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATtgtg                                                                                      <  1:626123/167‑1 (MQ=255)
                                                                                                                                                                                                                             aCCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATATGCTGCTGGCGGATTTCa    >  1:799659/1‑249 (MQ=255)
                                                                                                                                                                                                                             aCCGTAATCCGCAAATTCGGAAAATAGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATATGCTGCTGGCGGATTTCACt  <  1:402192/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 
CTGTTGCAACTGCTGGCGAAACGGCAACAGGTGGTGCACAAAGTGGGGGAAGTGAAACAGCAACACGGGTTGCCCATTATGCACCACAGCGTGAGGCGGAAATGTTGCAACGCAAGCGTGAGGCCGCCGAGCAAATGGGGTTATCTCCTAATCTGATTGAAGATGTGCTACGCCGCATTATGCGTGAATCCTATGTCAGCGAAAATCAATTCGGCTTTAAAACCGTAATCCGCAAATTCGGAAAATCGTTATCGTGGGCGGACGAGGCAAACTCGGCAGCTTATTCTGTCGTTATTTGAAGGGTTCCGGCTATCAGGTGAAATGTCTGGAGCGGGACGATTGGGCGCGCGCCGACCAAATTTTGCAGCATGCCGATGTGGTAATTGTGTCTGTGCCCATTGCCAACACGTTGGCGGTGATCGAACGGCTCAAACCTTATTTGACGGAAAATATGCTGCTGGCGGATTTCACT  >  1/1359342‑1359813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑