Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,357,179	A→G	L23P (CTT→CCT)	unknown ←	Putative NAD(P)H nitroreductase YdjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,357,179	0	A	G	100.0%	205.8 / NA	70	L23P (CTT→CCT)	unknown	Putative NAD(P)H nitroreductase YdjA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/42);  total (28/42)

ACTTTTTTAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAAGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAGTTATTTTTTCGATTATTAAATTTGCCTGGCGCACATTAAATTTTAT  >  1/1356939‑1357422                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aCTTTTTTAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTcggggc                                                                                                                                                                                                                                           <  1:578063/251‑1 (MQ=255)  cTTTTTTAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTcggggcg                                                                                                                                                                                                                                          >  1:896925/1‑251 (MQ=255)      tttAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCggggcggggg                                                                                                                                                                                                                                      <  1:127416/251‑1 (MQ=255)      tttAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCggggcggggg                                                                                                                                                                                                                                      <  1:112845/251‑1 (MQ=255)       ttAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCggggcgggggc                                                                                                                                                                                                                                     <  1:134302/251‑1 (MQ=255)                             ccaccaCGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTg                                                                                                                                                                                                               <  1:35416/251‑1 (MQ=255)                                     ccGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCC‑ATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTg                                                                                                                                                                                                      <  1:554133/251‑1 (MQ=255)                                          aaTCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAAc                                                                                                                                                                                                                 >  1:171961/1‑236 (MQ=255)                                          aaTCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGGTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTa                                                                                                                                                                                                  >  1:687931/1‑251 (MQ=255)                                                  gTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTaa                                                                                                                                                                                           <  1:676115/250‑1 (MQ=255)                                                  gTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAAt                                                                                                                                                                                          >  1:387316/1‑251 (MQ=255)                                                  gTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAAt                                                                                                                                                                                          >  1:189954/1‑251 (MQ=255)                                                   tGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATg                                                                                                                                                                                         <  1:85281/251‑1 (MQ=255)                                                                 gATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATg                                                                                                                                                                                         <  1:371665/237‑1 (MQ=255)                                                                     ttCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATcc                                                                                                                                                                       <  1:644226/251‑1 (MQ=255)                                                                     ttCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATcc                                                                                                                                                                       <  1:757330/251‑1 (MQ=255)                                                                            tttttCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAAt                                                                                                                                                                >  1:321522/1‑251 (MQ=255)                                                                                     cGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTACTGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAg                                                                                                                                                        <  1:609308/250‑1 (MQ=255)                                                                                       ttcttcGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTaa                                                                                                                                                                                           <  1:843095/213‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATaa                                                                                                                                         <  1:614581/250‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATaa                                                                                                                                         <  1:296404/250‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAAc                                                                                                                                        <  1:247412/251‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAAc                                                                                                                                        <  1:761917/251‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAAc                                                                                                                                        <  1:752679/251‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAAc                                                                                                                                        <  1:387417/251‑1 (MQ=255)                                                                                                    tCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTCGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAAc                                                                                                                                        <  1:445400/251‑1 (MQ=255)                                                                                                         ttCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGa                                                                                                                                                                                                                   <  1:671941/171‑1 (MQ=255)                                                                                                              gCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACAt                                                                                                                                      <  1:310927/243‑1 (MQ=255)                                                                                                                   gCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAAt                                                                                                                          <  1:479526/250‑1 (MQ=255)                                                                                                                       cTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTAAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAg                                                                                                                      >  1:454917/1‑250 (MQ=255)                                                                                                                               aaCTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGTGTTCATTTTCTTTATTCTTTTTATGTGAAAAGTGCGGCGGATTATAACATAg                                                                                                                                    >  1:826960/1‑228 (MQ=255)                                                                                                                                 cTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATg                                                                                                                                                                                         >  1:782189/1‑173 (MQ=255)                                                                                                                                  tCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGGTTCTTGAATTCAGCGGTTTATTGTa                                                                                                          >  1:462978/1‑251 (MQ=255)                                                                                                                                   cGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACAt                                                                                                                                      <  1:635149/222‑1 (MQ=255)                                                                                                                                   cGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTAc                                                                                                         <  1:810618/251‑1 (MQ=255)                                                                                                                                   cGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTAc                                                                                                         <  1:533499/251‑1 (MQ=255)                                                                                                                                   cGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTAc                                                                                                         <  1:617787/251‑1 (MQ=255)                                                                                                                                        aaGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACcggcg                                                                                                    <  1:623560/251‑1 (MQ=255)                                                                                                                                             aTTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATg                                                                                                                                                                                         >  1:188456/1‑161 (MQ=255)                                                                                                                                                cATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTg                                                                                                                                                                               <  1:107289/168‑1 (MQ=255)                                                                                                                                                cATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTgg                                                                                            >  1:758070/1‑251 (MQ=255)                                                                                                                                                   tttGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATCATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTAt                                                                                         <  1:147989/251‑1 (MQ=255)                                                                                                                                                          tGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTTCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGAc                                                                                  <  1:832870/251‑1 (MQ=255)                                                                                                                                                           gTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGTACTGCGTTCGGTGAGCAATTGTAATGCGTCCATTTGGTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATTGTTTCTTTAAttttgggggttattgt                                                                                                           >  1:673033/1‑211 (MQ=255)                                                                                                                                                           gTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGGGTTCGGGGAGTAATTGAAATGCGTCCATTTGCTTTTTCCTTTTAATGGGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTAAGCGGTTTATTTTACCGGCGGGTTGTGGTATTTTaaac                                                                                  >  1:476447/1‑247 (MQ=255)                                                                                                                                                           gTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATaa                                                                                                                                         <  1:799505/195‑1 (MQ=255)                                                                                                                                                           gTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGc                                                                                                                     >  1:173260/1‑215 (MQ=255)                                                                                                                                                           gTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGGCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAg                                                                                                                                    >  1:223515/1‑200 (MQ=255)                                                                                                                                                                    cGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTAt                                                                        >  1:411523/1‑251 (MQ=255)                                                                                                                                                                    cGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATGTTAGACGCCATTTTAt                                                                        <  1:703056/251‑1 (MQ=255)                                                                                                                                                                    cGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGGATTTTAGACGCCATTTTAt                                                                        >  1:74374/1‑251 (MQ=255)                                                                                                                                                                          aaCGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTg                                                                      <  1:516616/247‑1 (MQ=255)                                                                                                                                                                            cGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTg                                                                                                                                                                                              >  1:837809/1‑125 (MQ=255)                                                                                                                                                                            cGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGGATTTTAGACGCCATTTTATTGATTTc                                                                 >  1:496518/1‑250 (MQ=255)                                                                                                                                                                                    ttGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGtt                                                         <  1:884474/250‑1 (MQ=255)                                                                                                                                                                                         gTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGt                                                                                                                                                                                                       >  1:399348/1‑103 (MQ=255)                                                                                                                                                                                            tGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAg                                                <  1:617593/251‑1 (MQ=255)                                                                                                                                                                                              ccGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATaa                                                                                                                                         <  1:102028/160‑1 (MQ=255)                                                                                                                                                                                                            gcgTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGt                                                          <  1:742531/225‑1 (MQ=255)                                                                                                                                                                                                                 aTGCTGCCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAGTTATTTTTTCGATTATTAAAt                           >  1:264687/1‑251 (MQ=255)                                                                                                                                                                                                                      gcCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGGGGTTATATGAAAGTTATTTTTTCGATTATTAAATTTGc                       >  1:593629/1‑250 (MQ=255)                                                                                                                                                                                                                      gcCTGAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATg                                                    <  1:631251/221‑1 (MQ=255)                                                                                                                                                                                                                          gAAAGATCAGATCCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGGATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAGTTATTTTTTCGATTATTaaa                            >  1:895155/1‑241 (MQ=255)                                                                                                                                                                                                                              gatcagatcCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTgg                                                                                                                                                <  1:677353/121‑1 (MQ=255)                                                                                                                                                                                                                              gatcagatcCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTTAATTCAGCGGTTTATTGTACCGGCGGATTTTGGTATTTTAGACGCCATTTTTTTTATTTCCCGAGGGTATATGAAAGTTATTTTTTCGATTTTTTAATTTGCCTGGCGca               >  1:342727/1‑250 (MQ=255)                                                                                                                                                                                                                              gatcagatcCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTAtttt                                                                                      <  1:633827/179‑1 (MQ=255)                                                                                                                                                                                                                               atcagatcCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAGTTATTTTTTCGATTATTAAATTTGCCTGGCGcac              <  1:232715/250‑1 (MQ=255)                                                                                                                                                                                                                                   gatcCAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTgg                                                                                                                                                >  1:500313/1‑116 (MQ=255)                                                                                                                                                                                                                                       cAATTGTTGAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATaa                                                                                                                                         >  1:329281/1‑119 (MQ=255)                                                                                                                                                                                                                                          ttgttgAGGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAGTTATTTTTTCGATTATTAAATTTGCCTGGCGCACATTAAATTTTAt  >  1:732856/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ACTTTTTTAATGCTCGGATCCAATTTCGCCACCACGCCGATAATCATTGGTGCTCGGGTAGCCAGATTTTCCGCTTTTTTCAAGCGTTCTTCGCCAAGGTCGAATTCCTGCACGGCATCTTTTAGTAACTCGCTGAAGTTATTCATTTTGGCTTGTTCAATGACGATAAAACGATAAGGTTGCAGTTTGCCGTGATCCGGCGCGCGTAATGCTGCCTGAAAGATCAGATCCAATTGTTGAAGCGTCGGGGCGGGGGCGTTGAGTTTTTTTTCCGAACTGCGTTCGGTGAGTAATTGTAATGCGTCCATTTGCTTTATCCTTTTAATGTGAAAAGTGCGGTGGATTATAACATAGTTTCTTGAATTCAGCGGTTTATTGTACCGGCGGATTGTGGTATTTTAGACGCCATTTTATTGATTTCCCGAGGTTATATGAAAGTTATTTTTTCGATTATTAAATTTGCCTGGCGCACATTAAATTTTAT  >  1/1356939‑1357422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑