Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,343,029	G→A	A190V (GCC→GTC)	unknown ←	4‑alpha‑glucanotransferase (amylomaltase) (EC 2.4.1.25)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,343,029	0	G	A	100.0%	219.2 / NA	77	A190V (GCC→GTC)	unknown	4‑alpha‑glucanotransferase (amylomaltase) (EC 2.4.1.25)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/39);  total (38/39)

AATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGGCATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGATCGTGA  >  1/1342786‑1343271                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   aaTTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAAt                                                                                                                                                                                                                                             <  1:408064/251‑1 (MQ=255)     cGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCggg                                                                                                                                                                                                                                         >  1:705270/1‑251 (MQ=255)            ggcaaaATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAg                                                                                                                                                                                                                                   >  1:121130/1‑250 (MQ=255)              caaaATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGcc                                                                                                                                                                                                                                 <  1:307242/250‑1 (MQ=255)                         cccTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTg                                                                                                                                                                                                                      >  1:723973/1‑250 (MQ=255)                                         ccATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAg                                                                                                                                                                                                      >  1:121307/1‑250 (MQ=255)                                          cATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGcc                                                                                                                                                                                                    <  1:817187/251‑1 (MQ=255)                                              aCCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCcgcg                                                                                                                                                                                                >  1:451215/1‑251 (MQ=255)                                                   ggCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGcc                                                                                                                                                                                                    <  1:177350/242‑1 (MQ=255)                                                    gCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATaaa                                                                                                                                                                                          >  1:235543/1‑251 (MQ=255)                                                       tAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAAtt                                                                                                                                                                                        <  1:769449/250‑1 (MQ=255)                                                       tAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAAtt                                                                                                                                                                                        >  1:187279/1‑250 (MQ=255)                                                        aatcaatcCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCT‑GCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATc                                                                                                                                                                                                                        >  1:406720/1‑216 (MQ=255)                                                                          cgcgTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGt                                                                                                                                                                                                                                        <  1:352946/183‑1 (MQ=255)                                                                            cgTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGc                                                                                                                                                                   >  1:502792/1‑250 (MQ=255)                                                                                       cAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGt                                                                                                                                                       <  1:841401/251‑1 (MQ=255)                                                                                         aTTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTg                                                                                                                                                     <  1:20653/251‑1 (MQ=255)                                                                                                cTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCgg                                                                                                                                              >  1:180165/1‑251 (MQ=255)                                                                                                cTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCgg                                                                                                                                              >  1:241496/1‑251 (MQ=255)                                                                                                        ccgccgAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAgg                                                                                                                                      >  1:645310/1‑251 (MQ=255)                                                                                                           ccgAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGt                                                                                                                                    <  1:759768/250‑1 (MQ=255)                                                                                                             gAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATc                                                                                                                                                                                                                        <  1:598801/164‑1 (MQ=255)                                                                                                             gAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGtata                                                                                                                                 >  1:852023/1‑251 (MQ=255)                                                                                                                 tAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGc                                                                                                                             <  1:377147/251‑1 (MQ=255)                                                                                                                 tAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGc                                                                                                                             <  1:861473/251‑1 (MQ=255)                                                                                                                 tAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGc                                                                                                                             <  1:32438/251‑1 (MQ=255)                                                                                                                 tAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGc                                                                                                                             <  1:586194/251‑1 (MQ=255)                                                                                                                           ttGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTg                                                                                                                                                     <  1:869988/217‑1 (MQ=255)                                                                                                                           ttGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAAc                                                                                                                   <  1:408962/251‑1 (MQ=255)                                                                                                                           ttGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAAc                                                                                                                   <  1:58774/251‑1 (MQ=255)                                                                                                                                ccTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAggg                                                                                                                                     <  1:267803/228‑1 (MQ=255)                                                                                                                                ccTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGcc                                                                                                               >  1:882596/1‑250 (MQ=255)                                                                                                                                      cgcTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCagag                                                                                                         <  1:498470/250‑1 (MQ=255)                                                                                                                                                           gTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTg                                                                                                                                                     <  1:530187/185‑1 (MQ=255)                                                                                                                                                           gTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTg                                                                                                                                                     <  1:6229/185‑1 (MQ=255)                                                                                                                                                             tGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTc                                                                                 <  1:624725/251‑1 (MQ=255)                                                                                                                                                              gATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTc                                                                                 >  1:57317/1‑250 (MQ=255)                                                                                                                                                               aTCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGt                                                                                                                                                       <  1:684780/179‑1 (MQ=255)                                                                                                                                                               aTCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCg                                                                               >  1:582413/1‑251 (MQ=255)                                                                                                                                                                tCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAg                                                                                                                                                                                                      >  1:872309/1‑131 (MQ=255)                                                                                                                                                                  tATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGtt                                                                            <  1:898677/251‑1 (MQ=255)                                                                                                                                                                   aTGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGtt                                                                            >  1:565725/1‑250 (MQ=255)                                                                                                                                                                   aTGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGtt                                                                            >  1:440774/1‑250 (MQ=255)                                                                                                                                                                        gATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGgcgc                                                                                                                                                                                                         >  1:565542/1‑120 (MQ=255)                                                                                                                                                                         aTGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATaaca                                                                     >  1:325229/1‑251 (MQ=255)                                                                                                                                                                         aTGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATaac                                                                      <  1:441224/250‑1 (MQ=255)                                                                                                                                                                          tGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATaacaa                                                                    >  1:145370/1‑251 (MQ=255)                                                                                                                                                                             aaaTGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAggg                                                                                                                                     <  1:572110/183‑1 (MQ=255)                                                                                                                                                                             aaaTGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGc                                                                 <  1:140659/251‑1 (MQ=255)                                                                                                                                                                               atgatgTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACg                                                                  >  1:520772/1‑248 (MQ=255)                                                                                                                                                                                                     aTGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCaa                                         >  1:374502/1‑251 (MQ=255)                                                                                                                                                                                                          gggCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAACAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTa                                     >  1:197383/1‑250 (MQ=255)                                                                                                                                                                                                           ggCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCGCCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAGCTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTa                                     >  1:438478/1‑249 (MQ=255)                                                                                                                                                                                                           ggCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTAc                                    <  1:608155/250‑1 (MQ=255)                                                                                                                                                                                                             cTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCCCCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGc                                                                                                                             <  1:347411/159‑1 (MQ=255)                                                                                                                                                                                                             cTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActt                                  >  1:770338/1‑250 (MQ=255)                                                                                                                                                                                                             cTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActt                                  >  1:577983/1‑250 (MQ=255)                                                                                                                                                                                                             cTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActt                                  <  1:369298/250‑1 (MQ=255)                                                                                                                                                                                                             cTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActt                                  >  1:44755/1‑250 (MQ=255)                                                                                                                                                                                                               ataCGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGc                                                                                                                             >  1:386162/1‑157 (MQ=255)                                                                                                                                                                                                               ataCGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActtctt                               >  1:345868/1‑251 (MQ=255)                                                                                                                                                                                                                 aCGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActtcttct                             <  1:867151/251‑1 (MQ=255)                                                                                                                                                                                                                    ggCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAgg                                               >  1:786073/1‑230 (MQ=255)                                                                                                                                                                                                                    ggCTGGCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCtttt                          >  1:470366/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActtcttc                              >  1:732941/1‑243 (MQ=255)                                                                                                                                                                                                                         gCACCATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCAc                      <  1:463163/250‑1 (MQ=255)                                                                                                                                                                                                                            ccATCGGGATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTActtc                                 <  1:800415/236‑1 (MQ=255)                                                                                                                                                                                                                                   gATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAacac            <  1:511027/250‑1 (MQ=255)                                                                                                                                                                                                                                   gATTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTGGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAacac            <  1:426108/250‑1 (MQ=255)                                                                                                                                                                                                                                    aTTGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCAc                      >  1:265331/1‑239 (MQ=255)                                                                                                                                                                                                                                      tGCCCGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCAAGTTGCGCTCGGATTTCA‑GGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGAt       <  1:119635/251‑1 (MQ=255)                                                                                                                                                                                                                                        cccGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGAt       >  1:666092/1‑250 (MQ=255)                                                                                                                                                                                                                                        cccGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGAt       <  1:858952/250‑1 (MQ=255)                                                                                                                                                                                                                                         ccGGAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGATCg     <  1:135488/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGATCGTg   >  1:783896/1‑251 (MQ=255)                                                                                                                                                                                                                                            gAAAGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCACTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGATCGTGa  >  1:310080/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaaGAGGACATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATaac                                                                      <  1:761507/182‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   AATTCGGCAAAGGCAAAATGCAGCCCCTTCAATTTCAACGCCATCACCTCGGCGTAATCAATCCATTCGGCGGCGCGTAAGGCGTTCAATTTTTCCTGTACTTCCGCCGAATTAAACCAGCGTTGCGCCTCTGCGCTTTGTTGAAATTCCGGCAGTTGATCTATGTTGATGTAAATGATGTTCAGCCATTTGCGCGATGACGGGCTATACGGGCTGGCACCATCGGGATTGCCCGGAAAGAGGGCATGAATCGGGTTTAAGCCTAAAAAATCTGCCTGATACGGCGCAAGCCCGCGAATAAATTCTTGCAAATCACCGAAATCGCCCACGCCCCAGTTGCGCTCGGATTTCAGGGTATAAAGCTGAATAAAACTGCCCCAGAGTTTTTTCTGTTCGGCGAATGCTTCCGGTTCATAACAACGCTGCGGCGCAACGATAAGGCGGCAATGTACTTCTTCTTTTCCACCGTGCAACACCAGATCGTGA  >  1/1342786‑1343271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑