Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,341,473 T→C I10V (ATT→GTT)  unknown ← Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,341,4730TC100.0% 196.5 / NA 68I10V (ATT→GTT) unknownDihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/33);  total (35/33)

GAATCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAATGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACAGGGCGCTGTTGACC  >  1/1341229‑1341711
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              
gAATCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGttttt                                                                                                                                                                                                                                          >  1:837796/1‑251 (MQ=255)
  aTCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTaa                                                                                                                                                                                                                                        <  1:405282/251‑1 (MQ=255)
       aaTTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAAcc                                                                                                                                                                                                                                    >  1:150532/1‑250 (MQ=255)
       aaTTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAAcc                                                                                                                                                                                                                                    >  1:817220/1‑250 (MQ=255)
                    cTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAAc                                                                                                                                                                                                                                     >  1:75045/1‑236 (MQ=255)
                      ttcgtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCAtt                                                                                                                                                                                                                    <  1:58511/251‑1 (MQ=255)
                      ttcgtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCAtt                                                                                                                                                                                                                    <  1:645776/251‑1 (MQ=255)
                        cgtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTta                                                                                                                                                                                                                  <  1:375387/251‑1 (MQ=255)
                         gtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTtat                                                                                                                                                                                                                 >  1:759422/1‑251 (MQ=255)
                           ttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTatat                                                                                                                                                                                                               <  1:111967/251‑1 (MQ=255)
                           ttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTatat                                                                                                                                                                                                               <  1:786859/251‑1 (MQ=255)
                              gtggtgGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTgg                                                                                                                                                                                                            >  1:600466/1‑251 (MQ=255)
                               tggtggATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGt                                                                                                                                                                                                           >  1:803058/1‑251 (MQ=255)
                                     aTTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACAt                                                                                                                                                                                                      <  1:366704/250‑1 (MQ=255)
                                       tGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCAGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCgg                                                                                                                                                                                                   >  1:436858/1‑251 (MQ=255)
                                               ccAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAcc                                                                                                                                                                                           <  1:621841/251‑1 (MQ=255)
                                                caacaaTTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAccc                                                                                                                                                                                          <  1:241739/251‑1 (MQ=255)
                                                caacaaTTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAccc                                                                                                                                                                                          <  1:563345/251‑1 (MQ=255)
                                                      ttACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACg                                                                                                                                                                                    <  1:896875/251‑1 (MQ=255)
                                                       tACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTTAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGt                                                                                                                                                                                   <  1:434769/251‑1 (MQ=255)
                                                           aGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAg                                                                                                                                                                               >  1:468895/1‑251 (MQ=255)
                                                                  tCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCAc                                                                                                                                                                        <  1:95973/251‑1 (MQ=255)
                                                                      gTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCActtc                                                                                                                                                                     <  1:413650/250‑1 (MQ=255)
                                                                             aaaTTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAccc                                                                                                                                                                                          <  1:204475/222‑1 (MQ=255)
                                                                              aaTTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCaa                                                                                                                                                            <  1:87792/251‑1 (MQ=255)
                                                                                           aCTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGt                                                                                                                                                 >  1:307984/1‑249 (MQ=255)
                                                                                           aCTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGAAGGTGTTTAAAGTGCGGAGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGTGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGaa                                                                                                                                                    >  1:430053/1‑246 (MQ=255)
                                                                                                 aGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAACCGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATc                                                                                                                                          <  1:361295/250‑1 (MQ=255)
                                                                                                                         aTGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATc                                                                                                                                          >  1:352987/1‑226 (MQ=255)
                                                                                                                            cGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGtt                                                                                                              >  1:59473/1‑251 (MQ=255)
                                                                                                                              gTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTta                                                                                                                                                                                                                  >  1:754729/1‑149 (MQ=255)
                                                                                                                              gTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTaa                                                                                                            >  1:682375/1‑251 (MQ=255)
                                                                                                                                tCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAc                                                                                                          <  1:885429/251‑1 (MQ=255)
                                                                                                                                   ggTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAcgg                                                                                                        >  1:77619/1‑250 (MQ=255)
                                                                                                                                    gTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAcggcg                                                                                                      >  1:655398/1‑251 (MQ=255)
                                                                                                                                                  cTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGtt                                                                                                                                                                                                          <  1:284970/137‑1 (MQ=255)
                                                                                                                                                  cTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAcggctgt                                                                                                    <  1:177404/239‑4 (MQ=255)
                                                                                                                                                  cTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGt                                                                                        >  1:460212/1‑251 (MQ=255)
                                                                                                                                                       cTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGc                                                                                   <  1:424098/251‑1 (MQ=255)
                                                                                                                                                           ttggttggGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATAc                                                                               <  1:494216/251‑1 (MQ=255)
                                                                                                                                                             ggttggGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATa                                                                                >  1:181374/1‑248 (MQ=255)
                                                                                                                                                                 gggATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTg                                                                          >  1:560941/1‑250 (MQ=255)
                                                                                                                                                                   gATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAAt                                                                                                                                                   >  1:220652/1‑175 (MQ=255)
                                                                                                                                                                   gATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGcc                                                                        >  1:405165/1‑250 (MQ=255)
                                                                                                                                                                        gATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCg                                                                                         >  1:46138/1‑228 (MQ=255)
                                                                                                                                                                        gATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGAt                                                                  >  1:690501/1‑251 (MQ=255)
                                                                                                                                                                           gAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCActtc                                                                                                                                                                     >  1:72254/1‑149 (MQ=255)
                                                                                                                                                                           gAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATAc                                                                               >  1:100533/1‑235 (MQ=255)
                                                                                                                                                                               aCCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGAcc                                                           <  1:495371/251‑1 (MQ=255)
                                                                                                                                                                                ccccTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCg                                                          >  1:20574/1‑251 (MQ=255)
                                                                                                                                                                                  ccTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTgg                                                                            >  1:536671/1‑231 (MQ=255)
                                                                                                                                                                                         aCGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTCTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCa                                                 <  1:454251/251‑1 (MQ=255)
                                                                                                                                                                                              atttatttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTTTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGAATGTTGACCGGTTCGGTCATAccc                                            <  1:398778/251‑1 (MQ=255)
                                                                                                                                                                                              atttatttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATAccc                                            <  1:40178/251‑1 (MQ=255)
                                                                                                                                                                                                 tatttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACccagc                                         <  1:410974/251‑1 (MQ=255)
                                                                                                                                                                                                  atttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACccagc                                         >  1:326988/1‑250 (MQ=255)
                                                                                                                                                                                                         cGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTCACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTc                                 <  1:436111/251‑1 (MQ=255)
                                                                                                                                                                                                           ttGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCg                               <  1:42223/251‑1 (MQ=255)
                                                                                                                                                                                                             ggCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGt                             >  1:753716/1‑251 (MQ=255)
                                                                                                                                                                                                                  gggTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATAccc                                            >  1:620190/1‑231 (MQ=255)
                                                                                                                                                                                                                  gggTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGaa                        >  1:421535/1‑251 (MQ=255)
                                                                                                                                                                                                                        gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGtt                                                                                             <  1:140848/176‑1 (MQ=255)
                                                                                                                                                                                                                        gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTgg                                                                            >  1:177583/1‑193 (MQ=255)
                                                                                                                                                                                                                        gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTgg                                                                            <  1:132863/193‑1 (MQ=255)
                                                                                                                                                                                                                        gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATAccc                                            <  1:710469/225‑1 (MQ=255)
                                                                                                                                                                                                                         tgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGaa                  >  1:564335/1‑250 (MQ=255)
                                                                                                                                                                                                                          gtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACa                >  1:859132/1‑251 (MQ=255)
                                                                                                                                                                                                                                         ggTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACAGGGCGCTGTTGAcc  <  1:205417/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              
GAATCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAATGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACAGGGCGCTGTTGACC  >  1/1341229‑1341711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑