Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,330,393 G→A I27I (ATC→ATT)  unknown ← Lipid A export permease/ATP‑binding protein MsbA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,330,3930GA100.0% 193.6 / NA 67I27I (ATC→ATT) unknownLipid A export permease/ATP‑binding protein MsbA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/31);  total (36/31)

GAACATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACGATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAGACTGATTTG  >  1/1330147‑1330621
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    
gAACATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCa                                                                                                                                                                                                                                  >  1:547300/1‑251 (MQ=255)
 aaCATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCaa                                                                                                                                                                                                                                 >  1:763756/1‑251 (MQ=255)
          tgtTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCtt                                                                                                                                                                                                                         <  1:186619/250‑1 (MQ=255)
              tAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    <  1:76100/251‑1 (MQ=255)
              tAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGATTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    <  1:62329/251‑1 (MQ=255)
                aacaaacGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACgg                                                                                                                                                                                                                  >  1:337639/1‑251 (MQ=255)
                 acaaacGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACggg                                                                                                                                                                                                                 <  1:378109/251‑1 (MQ=255)
                        gcggcgCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatc                                                                                                                                                                                                           <  1:217689/250‑1 (MQ=255)
                                  gTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATaa                                                                                                                                                                                                >  1:825115/1‑251 (MQ=255)
                                   tcatcaCTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATaaa                                                                                                                                                                                               <  1:129899/251‑1 (MQ=255)
                                      tcaCTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGt                                                                                                                                                                                            >  1:859661/1‑251 (MQ=255)
                                         ctactTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTg                                                                                                                                                                                         >  1:808530/1‑251 (MQ=255)
                                             tttGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAgg                                                                                                                                                                                     <  1:622686/251‑1 (MQ=255)
                                                     agaCCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCAt                                                                                                                                                                              <  1:8136/250‑1 (MQ=255)
                                                      gaCCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCAtgg                                                                                                                                                                            >  1:367238/1‑251 (MQ=255)
                                                           aTGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatcat                                                                                                                                                                                                         >  1:513194/1‑217 (MQ=255)
                                                                  aCAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGt                                                                                                                                                                   <  1:547670/248‑1 (MQ=255)
                                                                     gTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatca                                                                                                                                                                                                          >  1:58337/1‑206 (MQ=255)
                                                                     gTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAg                                                                                                                                                                     >  1:459517/1‑243 (MQ=255)
                                                                      tAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:296735/250‑1 (MQ=255)
                                                                      tAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:669384/250‑1 (MQ=255)
                                                                      tAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATc                                                                                                                                                            <  1:187951/251‑1 (MQ=255)
                                                                             aaaTAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:176744/243‑1 (MQ=255)
                                                                             aaaTAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCat                                                                                                                                                      <  1:638148/250‑1 (MQ=255)
                                                                                           aTGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTc                                                                                                                                       <  1:630896/251‑1 (MQ=255)
                                                                                                      gagaATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAGTCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAgg                                                                                                                                                                    >  1:456370/1‑211 (MQ=255)
                                                                                                        gaATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             >  1:651763/1‑216 (MQ=255)
                                                                                                                 gcaCCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCAt                                                                                                                                                                              >  1:224163/1‑190 (MQ=255)
                                                                                                                    ccacAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    >  1:694018/1‑149 (MQ=255)
                                                                                                                    ccacAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:66681/204‑1 (MQ=255)
                                                                                                                    ccacAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGATTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAAc                                                                                                                                                                                              >  1:874769/1‑171 (MQ=255)
                                                                                                                           tACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    <  1:70653/142‑1 (MQ=255)
                                                                                                                             ccGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCat                                                                                                                                                      >  1:260839/1‑202 (MQ=255)
                                                                                                                             ccGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTa                                                                                                     <  1:289811/251‑1 (MQ=255)
                                                                                                                               gACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAtt                                                                                                   >  1:146937/1‑251 (MQ=255)
                                                                                                                                aCATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  <  1:497769/251‑1 (MQ=255)
                                                                                                                                 cATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCaaaaaa                                                                                                                          <  1:766175/226‑1 (MQ=255)
                                                                                                                                 cATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  >  1:844379/1‑250 (MQ=255)
                                                                                                                                      gTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTa                                                                                                     >  1:771174/1‑242 (MQ=255)
                                                                                                                                                          gCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  >  1:8571/1‑225 (MQ=255)
                                                                                                                                                              tGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTa                                                                                                     >  1:873658/1‑218 (MQ=255)
                                                                                                                                                              tGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCa                                                                    >  1:405028/1‑251 (MQ=255)
                                                                                                                                                              tGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCa                                                                    <  1:545895/251‑1 (MQ=255)
                                                                                                                                                                            tcCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatca                                                                                                                                                                                                          <  1:862255/103‑1 (MQ=255)
                                                                                                                                                                              cAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAAc                                                                                                                                                                                              >  1:412976/1‑113 (MQ=255)
                                                                                                                                                                              cAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAgcg                                                    >  1:189035/1‑251 (MQ=255)
                                                                                                                                                                              cAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAgcg                                                    >  1:432359/1‑251 (MQ=255)
                                                                                                                                                                                             tAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAAtt                                     <  1:96817/251‑1 (MQ=255)
                                                                                                                                                                                              aaGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  >  1:124659/1‑189 (MQ=255)
                                                                                                                                                                                                         gCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCa                         >  1:519313/1‑251 (MQ=255)
                                                                                                                                                                                                          ccGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTc                          <  1:424338/249‑1 (MQ=255)
                                                                                                                                                                                                             gAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGAt                                                                        <  1:536523/200‑1 (MQ=255)
                                                                                                                                                                                                              aaTCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGTGGTCTGAATTTTGATCTCTTCATTGt                     <  1:555009/250‑1 (MQ=255)
                                                                                                                                                                                                                 cGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAAttt                                    >  1:377782/1‑232 (MQ=255)
                                                                                                                                                                                                                   gTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAAc                <  1:339100/250‑1 (MQ=255)
                                                                                                                                                                                                                    ttAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACaa              <  1:649337/251‑1 (MQ=255)
                                                                                                                                                                                                                       aCGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAg           <  1:157186/251‑1 (MQ=255)
                                                                                                                                                                                                                        cgcgTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGGCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGAt                                >  1:16209/1‑229 (MQ=255)
                                                                                                                                                                                                                          cgTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTg                                                                                                                                         >  1:793327/1‑122 (MQ=255)
                                                                                                                                                                                                                          cgTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAAtt                                     >  1:275325/1‑222 (MQ=255)
                                                                                                                                                                                                                           gTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAgg                                                                                                                                                                    >  1:380380/1‑94 (MQ=255)
                                                                                                                                                                                                                                aTACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGt                                                                                                                                 >  1:766255/1‑124 (MQ=255)
                                                                                                                                                                                                                                aTACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGt                     >  1:242593/1‑232 (MQ=255)
                                                                                                                                                                                                                                 tACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAt                                                                                                    <  1:749814/152‑1 (MQ=255)
                                                                                                                                                                                                                                 tACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAGACTGATTTg  >  1:35965/1‑250 (MQ=255)
                                                                                                                                                                                                                                      aCGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGt                     >  1:688925/1‑226 (MQ=255)
                                                                                                                                                                                                                                           acacCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCcaatcaa                                                                                <  1:439856/162‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    
GAACATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACGATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAGACTGATTTG  >  1/1330147‑1330621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑