Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,319,579	C→G	Q141E (CAA→GAA)	unknown →	Selenide,water dikinase (EC 2.7.9.3)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,319,579	0	C	G	100.0%	229.7 / NA	80	Q141E (CAA→GAA)	unknown	Selenide,water dikinase (EC 2.7.9.3)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (44/36);  total (44/36)

ATTTATTGGTGGGTAACGAAACCAAAGATGATGCGGCAGTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCCAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAACAGGGCAAATTAAAACCGGAACA  >  1/1319346‑1319817                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               aTTTATTGGTGGGTAACGAAACCAAAGATGATGCGGCAGTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGAcgg                                                                                                                                                                                                                                >  1:764507/1‑250 (MQ=255) aTTTATTGGTGGGTAACGAAACCAAAGATGATGCGGCAGTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGAcgg                                                                                                                                                                                                                                >  1:125729/1‑250 (MQ=255)    tATTGGTGGGTAACGAAACCAAAGATGATGCGGCAGTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGt                                                                                                                                                                                                                            <  1:604211/251‑1 (MQ=255)               aaCGAAACCAAAGATGATGCGGCAGTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTg                                                                                                                                                                                                                 <  1:844563/251‑1 (MQ=255)                                       gTATATGATATTGGTCATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCAt                                                                                                                                                                                                  >  1:778887/1‑242 (MQ=255)                                       gTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCAttgttg                                                                                                                                                                                                                                    <  1:308362/208‑1 (MQ=255)                                       gTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTg                                                                                                                                                                                                                 >  1:235288/1‑227 (MQ=255)                                       gTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGccc                                                                                                                                                                                             <  1:83010/247‑1 (MQ=255)                                       gTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAg                                                                                                                                                                                          >  1:196704/1‑250 (MQ=255)                                        tataTGATATTGGTAATGGTATCGGGATCATCAGCAAAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGcc                                                                                                                                                                                        <  1:470405/251‑1 (MQ=255)                                              atatTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGa                                                                                                                                                                                  >  1:67696/1‑251 (MQ=255)                                                   ggTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGcc                                                                                                                                                                                        >  1:468477/1‑240 (MQ=255)                                                   ggTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACAtt                                                                                                                                                                              >  1:718040/1‑250 (MQ=255)                                                   ggTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGAGGT‑AAGCCGATAATGGCGATTGCGGCGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAg                                                                                                                                                                                                         >  1:12390/1‑223 (MQ=255)                                                      aaTGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCAt                                                                                                                                                                          <  1:841041/251‑1 (MQ=255)                                                        tGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTg                                                                                                                                                                        <  1:687207/251‑1 (MQ=255)                                                          gTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGAcggc                                                                                                                                                                                                                               >  1:269923/1‑194 (MQ=255)                                                          gTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCt                                                                                                                                                                                                                   >  1:330807/1‑206 (MQ=255)                                                          gTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGcc                                                                                                                                                                                        >  1:123559/1‑233 (MQ=255)                                                          gTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGAt                                                                                                                                                                      >  1:298431/1‑251 (MQ=255)                                                          gTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGAt                                                                                                                                                                      >  1:400422/1‑251 (MQ=255)                                                          gTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGAt                                                                                                                                                                      >  1:738727/1‑251 (MQ=255)                                                               gggATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGc                                                                                                                                                                  >  1:138963/1‑250 (MQ=255)                                                                  atcatcAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGAcggc                                                                                                                                                                                                                               >  1:188698/1‑186 (MQ=255)                                                                         gCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGAtt                                                                                                                                                       >  1:319640/1‑251 (MQ=255)                                                                            caacaGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCcggcgg                                                                                                                                                                                   >  1:49490/1‑220 (MQ=255)                                                                                  aTTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTCCCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGc                                                                                                                                                 <  1:585192/248‑1 (MQ=255)                                                                                      tttCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCgg                                                                                                                                          >  1:219516/1‑251 (MQ=255)                                                                                            gCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCg                                                                                                                                    >  1:750708/1‑251 (MQ=255)                                                                                                aTTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCat                                                                                                                                <  1:488755/251‑1 (MQ=255)                                                                                                   gTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCGGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCattat                                                                                                                             >  1:435355/1‑251 (MQ=255)                                                                                                   gTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTTGGCTTGGCGGTGACCGGCattat                                                                                                                             >  1:649195/1‑251 (MQ=255)                                                                                                   gTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCattat                                                                                                                             >  1:785412/1‑251 (MQ=255)                                                                                                    tCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCattatt                                                                                                                            >  1:350958/1‑251 (MQ=255)                                                                                                     cGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTg                                                                                                                           <  1:87495/251‑1 (MQ=255)                                                                                                       atgatCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGa                                                                                                                          <  1:834817/250‑1 (MQ=255)                                                                                                          atCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGTAAAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGcc                                                                                                                                                                                        >  1:132132/1‑186 (MQ=255)                                                                                                              tttCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAg                                                                                                                                                                                                         >  1:205148/1‑164 (MQ=255)                                                                                                               ttCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTg                                                                                                                                                                        >  1:65001/1‑196 (MQ=255)                                                                                                                         ggCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGccc                                                                                                                                                                <  1:671926/194‑1 (MQ=255)                                                                                                                          gCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCaaacga                                                                                                       <  1:845059/250‑1 (MQ=255)                                                                                                                                         tAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGcc                                                                                                                                                                                        >  1:453870/1‑154 (MQ=255)                                                                                                                                          aaCGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGc                                                                                      <  1:887763/251‑1 (MQ=255)                                                                                                                                              cAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTg                                                                                                                           >  1:795540/1‑210 (MQ=255)                                                                                                                                                     tGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAAcc                                                                           <  1:397262/251‑1 (MQ=255)                                                                                                                                                     tGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAAcc                                                                           <  1:701240/251‑1 (MQ=255)                                                                                                                                                     tGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAAcc                                                                           <  1:873404/251‑1 (MQ=255)                                                                                                                                                              cGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTaa                                                                  >  1:6035/1‑251 (MQ=255)                                                                                                                                                                cTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAAcc                                                                <  1:836702/251‑1 (MQ=255)                                                                                                                                                                  gtGGGTGGT‑GAGCGGATAATGGCGATTGCGGTTTTAGGATTACCGATTCCTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTAtt                                                                     <  1:378173/243‑1 (MQ=255)                                                                                                                                                                  aggggTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTCCCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGAcc                                                                                                                                     <  1:279480/178‑1 (MQ=255)                                                                                                                                                                  aTGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTCCCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAAc                                                                 <  1:83729/248‑1 (MQ=255)                                                                                                                                                                  aTGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTg                                                                                                                           >  1:859997/1‑190 (MQ=255)                                                                                                                                                                      gtggt‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAAcc                                                           <  1:100073/250‑1 (MQ=255)                                                                                                                                                                      gtggt‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAAc                                                            >  1:776511/1‑249 (MQ=255)                                                                                                                                                                              gCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCa                                                   >  1:621884/1‑251 (MQ=255)                                                                                                                                                                               ccGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTCCCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGc                                                    <  1:442377/249‑1 (MQ=255)                                                                                                                                                                               ccGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGc                                                    <  1:557086/249‑1 (MQ=255)                                                                                                                                                                               ccGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGc                                                    <  1:753806/249‑1 (MQ=255)                                                                                                                                                                               ccGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCAt                                                  >  1:684067/1‑251 (MQ=255)                                                                                                                                                                                cGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGcc                                                                                                                                                                                        >  1:187821/1‑116 (MQ=255)                                                                                                                                                                                  aTAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAg                                                                                                                                                                                                         <  1:335303/97‑1 (MQ=255)                                                                                                                                                                                            aacagttgtTAAGATTTCCGATTTCTAAATATCCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACt                                     <  1:507646/245‑1 (MQ=255)                                                                                                                                                                                               cGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTAc                                   <  1:670227/250‑1 (MQ=255)                                                                                                                                                                                                 gtgtTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAg                                                                                                                                                                                                         >  1:125246/1‑82 (MQ=255)                                                                                                                                                                                                 gtgtTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAAc                                                                 <  1:842231/218‑1 (MQ=255)                                                                                                                                                                                                 gtgtTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTAcgg                                 >  1:752987/1‑250 (MQ=255)                                                                                                                                                                                                   gtTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAAc                                                                 >  1:872962/1‑216 (MQ=255)                                                                                                                                                                                                     tAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGaa                            >  1:575829/1‑251 (MQ=255)                                                                                                                                                                                                       ggATTTCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGAcc                                                                                                                                     <  1:196962/144‑1 (MQ=255)                                                                                                                                                                                                         ttttCCGATTCCTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAAc                        <  1:504388/250‑1 (MQ=255)                                                                                                                                                                                                           ttCCGATTACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACt                                     <  1:743120/236‑1 (MQ=255)                                                                                                                                                                                                                 ttACTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAACAGGGCaaa                <  1:350793/251‑1 (MQ=255)                                                                                                                                                                                                                   aCTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAACAGGGCAAAtt              <  1:653813/251‑1 (MQ=255)                                                                                                                                                                                                                    cTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAg                                                                                          <  1:175094/174‑1 (MQ=255)                                                                                                                                                                                                                    cTAAATTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAAcc                                                                >  1:514150/1‑200 (MQ=255)                                                                                                                                                                                                                      aaaTTACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCaaacga                                                                                                       >  1:368932/1‑159 (MQ=255)                                                                                                                                                                                                                         ttACCCGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAACAGGGCAAATTAAAAcc        <  1:193850/251‑1 (MQ=255)                                                                                                                                                                                                                            cccGCCGAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAACAGGGCAAATTAAAAc         >  1:859129/1‑247 (MQ=255)                                                                                                                                                                                                                              cgccgAGGTTGCCGAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGC‑TCGGCGTTTTAACTACGGCGGCAAAACAGGGCAAATTAAAACCGGAaca  >  1:574361/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               ATTTATTGGTGGGTAACGAAACCAAAGATGATGCGGCAGTATATGATATTGGTAATGGTATCGGGATCATCAGCACAACAGATTTTTTCATGCCAATTGTCGATGATCCTTTCGATTTTGGGCGTATTGCGGCAACTAACGCAATTAGTGACATTTTCGCTATGGGTGGT‑AAGCCGATAATGGCGATTGCGGTGTTAGGATTTCCGATTACTAAATTACCCGCCGAGGTTGCCCAACGCATTGTTGACGGCGGTCGTTTCGCTTGTCAGGAAGCGGGCATTGCCCTAGCCGGCGGACATTCCATTGATTCGCCCGAACCGATTTTCGGCTTGGCGGTGACCGGCATTATTGATACGGAAAAAGTCAAACGAAACGCCTCCGCAGAAGCCGGTTGCAACCTGTATTTAACCAAACCTCTGGGCATCGGCGTTTTAACTACGGCGGAAAAACAGGGCAAATTAAAACCGGAACA  >  1/1319346‑1319817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑