Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,307,970	T→C	A579A (GCT→GCC)	unknown →	Helicase, SNF2/RAD54 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,307,970	0	T	C	100.0%	232.3 / NA	76	A579A (GCT→GCC)	unknown	Helicase, SNF2/RAD54 family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/40);  total (36/40)

GGGCAGAAATTTGACGTAGTTGTGGTGAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCTGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAGGTGTGTCGATAGGGCGTTATTTG  >  1/1307725‑1308204                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           gggCAGAAATTTGACGTAGTTGTGGTGAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATaa                                                                                                                                                                                                                                       >  1:473545/1‑251 (MQ=255)       aaaTTTGACGTAGTTGTGGTGAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAAtt                                                                                                                                                                                                                                   <  1:854358/249‑1 (MQ=255)                   gTTGTGGTGAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATaaa                                                                                                                                                                                                                     >  1:285887/1‑251 (MQ=255)                     tgtgGTGAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAg                                                                                                                                                                                                                   <  1:216438/251‑1 (MQ=255)                       tggtgAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGa                                                                                                                                                                                                                  <  1:878434/250‑1 (MQ=255)                         gtgAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGa                                                                                                                                                                                                                  <  1:604038/248‑1 (MQ=255)                                          aCGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTgg                                                                                                                                                                                              <  1:818392/251‑1 (MQ=255)                                          aCGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTgg                                                                                                                                                                                              <  1:782140/251‑1 (MQ=255)                                          aCGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTgg                                                                                                                                                                                              <  1:722230/251‑1 (MQ=255)                                          aCGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTgg                                                                                                                                                                                              <  1:392949/251‑1 (MQ=255)                                                                 tgtTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTa                                                                                                                                                                       >  1:118878/1‑251 (MQ=255)                                                                 tgtTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAATGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAg                                                                                                                                                                                            <  1:837085/230‑1 (MQ=255)                                                                    tAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    <  1:490726/251‑1 (MQ=255)                                                                    tAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    <  1:746730/251‑1 (MQ=255)                                                                    tAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    <  1:488879/251‑1 (MQ=255)                                                                    tAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    <  1:889984/251‑1 (MQ=255)                                                                    tAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    <  1:459014/251‑1 (MQ=255)                                                                        gTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    >  1:331016/1‑247 (MQ=255)                                                                           acttacCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTaa                                                                                                                                                             >  1:646695/1‑251 (MQ=255)                                                                              tacCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAg                                                                                                                                                          <  1:15575/251‑1 (MQ=255)                                                                                                tttCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCGTAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGtt                                                                                                                                        >  1:241913/1‑251 (MQ=255)                                                                                                    cAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAg                                                                                                                                    <  1:381472/251‑1 (MQ=255)                                                                                                     aaGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGt                                                                                                                                   <  1:262667/251‑1 (MQ=255)                                                                                                        gtgtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATaaa                                                                                                                                                                                                                     >  1:777021/1‑166 (MQ=255)                                                                                                        gtgtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAg                                                                                                                                                          >  1:563828/1‑225 (MQ=255)                                                                                                        gtgtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCg                                                                                                                                >  1:418024/1‑251 (MQ=255)                                                                                                          gtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGa                                                                                                                                                                                                                  >  1:248485/1‑167 (MQ=255)                                                                                                          gtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAg                                                                                                                                                                                            <  1:209300/189‑1 (MQ=255)                                                                                                          gtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    <  1:306043/213‑1 (MQ=255)                                                                                                          gtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    >  1:99942/1‑213 (MQ=255)                                                                                                          gtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAg                                                                                                                                                          >  1:310033/1‑223 (MQ=255)                                                                                                          gtgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGAt                                                                                                                              >  1:698904/1‑251 (MQ=255)                                                                                                           tgtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATg                                                                                                                             >  1:642522/1‑251 (MQ=255)                                                                                                            gtTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGAt                                                                                                                                                                    >  1:28002/1‑211 (MQ=255)                                                                                                                    cTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATgg                                                                                                                    <  1:702205/251‑1 (MQ=255)                                                                                                                       cTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATgg                                                                                                                                                    >  1:553829/1‑216 (MQ=255)                                                                                                                       cTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTc                                                                                                                 <  1:112851/251‑1 (MQ=255)                                                                                                                         gatgaAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTgg                                                                                                                                                                                              >  1:559587/1‑172 (MQ=255)                                                                                                                         gatgaAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGGGAGCg                                                                                                                                          >  1:390074/1‑224 (MQ=255)                                                                                                                         gatgaAGTGCTGGAGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGtt                                                                                                                  >  1:729918/1‑248 (MQ=255)                                                                                                                            gaAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTgg                                                                                                                                                                                              <  1:449672/169‑1 (MQ=255)                                                                                                                             aaGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTgggg                                                                                                            <  1:864680/250‑1 (MQ=255)                                                                                                                             aaGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTgggg                                                                                                            <  1:359478/250‑1 (MQ=255)                                                                                                                             aaGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTgggg                                                                                                            <  1:346342/250‑1 (MQ=255)                                                                                                                             aaGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTgggg                                                                                                            <  1:594353/250‑1 (MQ=255)                                                                                                                               gTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGgtgg                                                                                                         >  1:269041/1‑250 (MQ=255)                                                                                                                                  cTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGa                                                                                                       <  1:221588/250‑1 (MQ=255)                                                                                                                                      ggCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGa                                                                                                       >  1:327755/1‑246 (MQ=255)                                                                                                                                         aGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAAttt                                                                                               >  1:562934/1‑251 (MQ=255)                                                                                                                                          gATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTg                                                                                              >  1:228034/1‑251 (MQ=255)                                                                                                                                            ttGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGcc                                                                                            <  1:130615/251‑1 (MQ=255)                                                                                                                                                   gggCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGgcg                                                                                                                                                  <  1:402013/190‑1 (MQ=255)                                                                                                                                                    ggCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGAc                                                                                                                                                                                                                             >  1:194056/1‑114 (MQ=255)                                                                                                                                                    ggCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGCGATGGCGCGTGAGCGTTTGAGTGCGAt                                                                                                                              >  1:739058/1‑209 (MQ=255)                                                                                                                                                            tatTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCgg                                                                            <  1:484079/251‑1 (MQ=255)                                                                                                                                                            tatTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCgg                                                                            <  1:832319/251‑1 (MQ=255)                                                                                                                                                                    aTCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAAc                                                                    <  1:349014/251‑1 (MQ=255)                                                                                                                                                                    aTCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAAc                                                                    <  1:743861/251‑1 (MQ=255)                                                                                                                                                                            gCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAAc                                                                                                                         >  1:637420/1‑190 (MQ=255)                                                                                                                                                                               aaCATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTg                                                         <  1:616826/251‑1 (MQ=255)                                                                                                                                                                                 cATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTgggg                                                                                                            <  1:501569/198‑1 (MQ=255)                                                                                                                                                                                 cATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGt                                                       >  1:332587/1‑251 (MQ=255)                                                                                                                                                                                  aTTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAAc                                                                    >  1:703282/1‑237 (MQ=255)                                                                                                                                                                                    ttATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATgg                                                                                                                            >  1:77271/1‑179 (MQ=255)                                                                                                                                                                                       tGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGTATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCt                                                 <  1:434002/251‑1 (MQ=255)                                                                                                                                                                                         ccACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTa                                               <  1:187444/251‑1 (MQ=255)                                                                                                                                                                                                       gagaAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGc                                  >  1:219631/1‑250 (MQ=255)                                                                                                                                                                                                       gagaAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGc                                  >  1:511719/1‑250 (MQ=255)                                                                                                                                                                                                       gagaAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGc                                  >  1:586451/1‑250 (MQ=255)                                                                                                                                                                                                              aaaTTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGcc                                                                                            >  1:559727/1‑185 (MQ=255)                                                                                                                                                                                                                          ccTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAg                                                                                                                                                                                                                   >  1:125373/1‑54 (MQ=255)                                                                                                                                                                                                                          ccTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAGGTGTGTCGATa              >  1:810260/1‑251 (MQ=255)                                                                                                                                                                                                                          ccTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAGGTGTGTCGATa              >  1:810368/1‑251 (MQ=255)                                                                                                                                                                                                                          ccTTTGATGAGTTACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTGTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAGGTGTGTCGAt               <  1:432004/250‑1 (MQ=255)                                                                                                                                                                                                                                      tACAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAGGTGTGTCGATAGGGCGTTATTTg  <  1:542783/251‑1 (MQ=255)                                                                                                                                                                                                                                        cAAGCGAAATTTGCCGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAgg                        <  1:53668/227‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           GGGCAGAAATTTGACGTAGTTGTGGTGAATTTCTTGAATAAACGCAATCTTGCCGATCAACGCGTGTTAGAGTTACTTACCGAGAAATTTAAACTTTTCCAAGGTGTGTTAGGGGCTTCTGATGAAGTGCTGGGGCAGATTGAATCGGGCGTAGATATTGAAAATCAAATAGCCAACATTTATGCCACTTGTCGAACAGAGAAAGAAATTCAGCATGCCTTTGATGAGTTACAAGCGAAATTTGCTGATAAAATTAGCGACCGCATAAAAGAAACGCAAGAAGCCTTAGTGGAGCGTTTTGATCAGGCGGTTTTAGATCGTCTAAAAGTGATGGCGCGTGAGCGTTTGAGTGCGATGGAACAATGGTTCTGGGGTGTGACGCAATTTGCCTTGGCAGAGAAAGCGGTGTTTAACCATTCGGATTGGTATTTCTTATTGAAAGATTCGCCGGTGGGAGGTGTGTCGATAGGGCGTTATTTG  >  1/1307725‑1308204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑