Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,302,646 C→A P279H (CCT→CAT)  unknown → Type III restriction‑modification system methylation subunit (EC 2.1.1.72)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,302,6460CA95.5% 53.9 / ‑4.4 22P279H (CCT→CAT) unknownType III restriction‑modification system methylation subunit (EC 2.1.1.72)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (7/14);  minor base G (0/1);  total (7/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GCACTTGGTGGTGGATACGTCGCTTTTTGAGCCAAGTTTTCAGGCAGATATATTAAAAAACATTGAAGATCTAGATGAACAAACTAATGGCTTATTAATTCATTCCGATAATTTCCAAGCATTAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCCTCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAAT  >  1/1302410‑1302861
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                       
gCACTTGGTGGTGGATACGTCGCTTTTTGAGCCAAGTTTTCAGGCAGATATATTAAAAAACATTGAAGATCTAGATGAACAAACTAATGGCTTATTAATTCATTCCGATAATTTCCAAGCATTAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATggg                                                                                                                                                                                                           >  1:129838/1‑251 (MQ=255)
        tggtggATACGTCGCTTTTTGAGCCAAGTTTTCAGGCAGATATATTAAAAAACATTGAAGATCTAGATGAACAAACTAATGGCTTATTAATTCATTCCGATAATTTCCAAGCATTAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCttt                                                                                                                                                                                                    <  1:404538/250‑1 (MQ=255)
                                                                                            tattaATTCATTCCGATAATTTCCAAGCATTAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATg                                                                                                                                                                                                             <  1:40407/157‑1 (MQ=255)
                                                                                                                       cATTAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATAc                                                                                     <  1:596201/250‑1 (MQ=255)
                                                                                                                         ttAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATtgatg                                                                                                                             >  1:182174/1‑208 (MQ=255)
                                                                                                                                            gATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAg                                                                                                                                                                                <  1:741165/138‑1 (MQ=255)
                                                                                                                                            gATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATtgatg                                                                                                                             >  1:256955/1‑189 (MQ=255)
                                                                                                                                            gATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATgaga                                                                                                                    <  1:887312/198‑1 (MQ=255)
                                                                                                                                            gATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATg                                                                                                                       <  1:8893/195‑1 (MQ=255)
                                                                                                                                             atatAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTc                                                                                                                                                                                                                   <  1:361707/102‑1 (MQ=255)
                                                                                                                                                                 gTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATg                                                                                                                                                                                                             <  1:368111/88‑1 (MQ=255)
                                                                                                                                                                 gTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCAt                                                                                                                                                                   >  1:434224/1‑130 (MQ=255)
                                                                                                                                                                      tataTTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCttt                                                                                                                                                                                                    <  1:852979/92‑1 (MQ=255)
                                                                                                                                                                           tGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCGTCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAaccacac                                <  1:570972/251‑1 (MQ=255)
                                                                                                                                                                               ccgccgTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATc                                                                                                               <  1:514880/168‑1 (MQ=255)
                                                                                                                                                                                 gccgTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTa                          >  1:798706/1‑251 (MQ=255)
                                                                                                                                                                                              gAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGa                                                                                                                                                       >  1:486267/1‑113 (MQ=255)
                                                                                                                                                                                              gAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGAt                                                                                                                   <  1:16440/149‑1 (MQ=255)
                                                                                                                                                                                              gAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAAt                                                                                                            <  1:203400/156‑1 (MQ=255)
                                                                                                                                                                                                                         gTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAAt  <  1:602467/235‑1 (MQ=255)
                                                                                                                                                                                                                           cTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTtatat                                                                                                                                                                                             >  1:324100/1‑46 (MQ=255)
                                                                                                                                                                                                                                    tGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATg                                                                                                                                                     <  1:516599/77‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                       
GCACTTGGTGGTGGATACGTCGCTTTTTGAGCCAAGTTTTCAGGCAGATATATTAAAAAACATTGAAGATCTAGATGAACAAACTAATGGCTTATTAATTCATTCCGATAATTTCCAAGCATTAAATTTATTGCAGGCAAGATATAAAGAACAGGTGAAATGTATTTATATTGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCCTCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAAT  >  1/1302410‑1302861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑