Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,299,379	G→A	A116T (GCG→ACG)	unknown →	Translation initiation factor 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,299,379	0	G	A	100.0%	177.2 / NA	63	A116T (GCG→ACG)	unknown	Translation initiation factor 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/34);  total (29/34)

CAGGTGGAAGTGCGTAAAAAGCGCACTATTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAGCGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTCGCGGCAAAAATAAAGTGACCAAAGC  >  1/1299136‑1299623                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     cAGGTGGAAGTGCGTAAAAAGCGCACTATTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGgaaga                                                                                                                                                                                                                                               >  1:66963/1‑251 (MQ=255)             cGTAAAAAGCGCACTATTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTAcg                                                                                                                                                                                                                                   >  1:895031/1‑251 (MQ=255)                      cgcACTATTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAg                                                                                                                                                                                                                           <  1:73808/250‑1 (MQ=255)                          cTATTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGAt                                                                                                                                                                                                                      >  1:29967/1‑251 (MQ=255)                            aTTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTAcgt                                                                                                                                                                                                                                  >  1:865517/1‑237 (MQ=255)                            aTTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGa                                                                                                                                                                                                                    >  1:513133/1‑251 (MQ=255)                            aTTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGa                                                                                                                                                                                                                    >  1:56221/1‑251 (MQ=255)                            aTTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGa                                                                                                                                                                                                                    >  1:391055/1‑251 (MQ=255)                               ccGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGtt                                                                                                                                                                                                                 <  1:771181/251‑1 (MQ=255)                                 gACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTAcgt                                                                                                                                                                                                                                  >  1:656987/1‑232 (MQ=255)                                      aaGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGccc                                                                                                                                                                                                            <  1:504930/249‑1 (MQ=255)                                      aaGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGcc                                                                                                                                                                                                             <  1:824122/248‑1 (MQ=255)                                         ccGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCaa                                                                                                                                                                                                       >  1:667885/1‑251 (MQ=255)                                             ccaAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGGGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAg                                                                                                                                                                                                   <  1:337334/250‑1 (MQ=255)                                                                   aaaTTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAagc                                                                                                                                                                             <  1:320643/251‑1 (MQ=255)                                                                         aaaGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGcagcag                                                                                                                                                                        <  1:327356/250‑1 (MQ=255)                                                                             ccAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAgaagaa                                                                                                                                                                   >  1:795703/1‑251 (MQ=255)                                                                                gACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAgaagcg                                                                                                                                                                >  1:224506/1‑251 (MQ=255)                                                                                            aGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGaagaag                                                                                                                                                                  <  1:667655/237‑1 (MQ=255)                                                                                            aGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTAcgc                                                                                                                                                     <  1:51033/250‑1 (MQ=255)                                                                                             gCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCgg                                                                                                                                                   >  1:694141/1‑251 (MQ=255)                                                                                                        tAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCga                                                                                                                                         <  1:615132/250‑1 (MQ=255)                                                                                                          gaagaaAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCgatga                                                                                                                                      >  1:184447/1‑251 (MQ=255)                                                                                                               aaaaGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATc                                                                                                                                 <  1:148608/251‑1 (MQ=255)                                                                                                                        aaGAGAGAAGGCGGTGAAAG‑AAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAg                                                                                                                       <  1:32869/251‑1 (MQ=255)                                                                                                                         agagagAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAAc                                                                                                                                                <  1:866590/226‑1 (MQ=255)                                                                                                                          gagagaAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGcc                                                                                                                                                                                                             <  1:445734/164‑1 (MQ=255)                                                                                                                          gagagaAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAAc                                                                                                                                                >  1:335992/1‑225 (MQ=255)                                                                                                                          gagagaAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCga                                                                                                                                         <  1:722558/232‑1 (MQ=255)                                                                                                                          gagagaAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAg                                                                                                                       >  1:876886/1‑250 (MQ=255)                                                                                                                           agagaAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAg                                                                                                                       <  1:438606/249‑1 (MQ=255)                                                                                                                             agaAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATTAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCaa                                                                                                                    <  1:438831/250‑1 (MQ=255)                                                                                                                                  gCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACgg                                                                                                               <  1:512880/250‑1 (MQ=255)                                                                                                                                         aagaaaaagaaGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTa                                                                                                        >  1:305698/1‑250 (MQ=255)                                                                                                                                            aaaaagaaGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTc                                                                                                     <  1:66980/250‑1 (MQ=255)                                                                                                                                             aaaagaaGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCaga                                                                                                                                                                       >  1:576285/1‑183 (MQ=255)                                                                                                                                                     ccGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAAt                                                                                           <  1:766189/251‑1 (MQ=255)                                                                                                                                                              aaGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACgg                                                                                                               <  1:124267/222‑1 (MQ=255)                                                                                                                                                                   aaaCAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAAt                                                                                                                                                                                                                                       <  1:401255/97‑1 (MQ=255)                                                                                                                                                                        ggcacaggcCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGGGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGcc                                                                         >  1:693482/1‑250 (MQ=255)                                                                                                                                                                              ggcCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGaagaag                                                                                                                                                                  <  1:637131/155‑1 (MQ=255)                                                                                                                                                                              ggcCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGaa                                                                   <  1:420503/250‑1 (MQ=255)                                                                                                                                                                                   cTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGaa                                                             >  1:865224/1‑251 (MQ=255)                                                                                                                                                                                   cTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGaa                                                             >  1:708524/1‑251 (MQ=255)                                                                                                                                                                                       ggCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATAc                                                                                                                 >  1:530056/1‑195 (MQ=255)                                                                                                                                                                                       ggCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTc                                                                                                     <  1:306572/207‑1 (MQ=255)                                                                                                                                                                                               gTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAagag                                                 >  1:898584/1‑251 (MQ=255)                                                                                                                                                                                                tGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAagag                                                 <  1:239011/250‑1 (MQ=255)                                                                                                                                                                                                    gTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAg                                                                                                            >  1:41484/1‑187 (MQ=255)                                                                                                                                                                                                    gTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGa                                                                    >  1:74551/1‑227 (MQ=255)                                                                                                                                                                                                    gTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGcgccg                                            >  1:768998/1‑251 (MQ=255)                                                                                                                                                                                                         taatCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATg                                       <  1:22469/251‑1 (MQ=255)                                                                                                                                                                                                               aGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGc                                                                          >  1:681308/1‑210 (MQ=255)                                                                                                                                                                                                               aGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGa                                                              <  1:144770/222‑1 (MQ=255)                                                                                                                                                                                                               aGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGt                                  >  1:504722/1‑250 (MQ=255)                                                                                                                                                                                                                gTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGGAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACaa                                                                                            >  1:596674/1‑191 (MQ=255)                                                                                                                                                                                                                    aaTCTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGt                            <  1:868505/251‑1 (MQ=255)                                                                                                                                                                                                                       cTGTGGATCCGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTcg                          >  1:615989/1‑250 (MQ=255)                                                                                                                                                                                                                                cGGAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTCGCGGCAAAAAt                <  1:98452/251‑1 (MQ=255)                                                                                                                                                                                                                                  gAAAAAGAGAAACGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGccc                                                                        <  1:30874/193‑1 (MQ=255)                                                                                                                                                                                                                                           aaaCGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTCGCGGCAAAAATAAAGTGAcc       <  1:142656/249‑1 (MQ=255)                                                                                                                                                                                                                                             aCGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTCGCGGCAAAAATAAAGTGACCAAAg   <  1:154556/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGCAAAACGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTCGCGGCAAAAATAAAGTGACCAAAGc  <  1:767466/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CAGGTGGAAGTGCGTAAAAAGCGCACTATTCCGACAGAAGCCGCACAAAAAGCCGAAGAAGCGGCAAAATTGAAAGCCAGACAAGAAGCCGAGCAAAAAGCGGTAGAAGAAAAAGTGCAAAGAGAGAAGGCGGTGAAAGAAAAAGAAGCCGCTGAAAAAGCAAAACAGGCACAGGCCACTAAGGCACAAAGTGCGGTCAATAATCAAGTTAAATCTGTGGATCCGGAAAAAGAGAAACGCAAAGCGGAAGAAGCGGAATTACGTCGTAAAGCGGATGAGTTAGCCCGCCAAAAAGCGGAAGAACAAGCGCGTAAAGCAGCAGAAGAAGCGAAGCGTTACGCGGAACTTTCCGATGATAATCAGCACAACAGCAATACGGAAGATTACTCCGATTACAATTTAACCTCCAGTTATGCCCTGGAAGCGGAAGATGAAGAAGAGCGCCGCAATGAAGGTCGTGGTCGCGGCAAAAATAAAGTGACCAAAGC  >  1/1299136‑1299623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑